The reeler gene encodes a protein with an EGF–like motif expressed by pioneer neurons
Abstract
We have identified a strong candidate cDNA for the mouse reeler gene. This 5 kb transcript encodes a 99.4 kD protein consisting of 881 amino acids and possessing two EGF-like motifs. We assayed two independent mutant alleles--'Jackson reeler', which has a deletion of the entire gene, and 'Orleans reeler' which exhibits a 220 bp deletion in the open reading frame, including the second EGF-like motif and resulting in a frame shift. In situ hybridization reveals that the transcript is detected exclusively in the pioneer neurons which guide neuronal cell migration along the radial array. Our findings offer an explanation for how the reeler mutant phenotype causes a disturbance of the complex architecture of the neuronal network.
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VLDLR and ApoER2 have a critical role in the neuronal Reelin pathway essential for proper neuronal positioning and brain development.
“studies in recent years have established a critical role for VLDLR and ApoER2 in the neuronal Reelin pathway that is essential for proper neuronal positioning and brain development (16–19).”
From The E3 ubiquitin ligase IDOL induces the degradation of the low density lipoprotein receptor family members VLDLR and ApoER2 (2010) · cites this paper as [19]
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- Authors
- Hirotsune, S., Takahara, T., Sasaki, N., Hirose, K., Yoshiki, A., Ohashi, T., Kusakabe, M., Murakami, Y., Muramatsu, M., Watanabe, S., Nakao, K., Katsuki, M., & Hayashizaki, Y.
- Affiliations
- RIKEN (JP)
- RIKEN BioResource Research Center (JP)
- Shionogi (Japan) (JP)
- Kyushu University (JP)
- Venue
- Nature Genetics
- Published
- 1995-05
- Volume
- 10
- Issue
- 1
- Pages
- 77-83
- ISSN
- 1061-4036 (ISSN-L)
- 1546-1718
- OpenAlex
- W2022888964
- Cited by
- 345
- References
- 1
- Citations by year
- 2026: 3 · 2025: 3 · 2024: 2 · 2023: 4 · 2022: 8 · 2021: 6 · 2020: 6 · 2019: 4 · 2018: 5 · 2017: 5 · 2016: 8 · 2015: 8 · 2014: 8 · 2013: 7 · 2012: 4
- FWCI
- 9.92
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Nature Genetics (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Axon Guidance and Neuronal Signaling
- Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Keywords
- pioneer neurons, neuronal migration, radial migration, frame shift, cDNA cloning, mice
- MeSH terms
- Reelin Protein, Amino Acid Sequence, Animals, Animals, Newborn, Base Sequence, Brain, Chromosome Mapping, Cloning, Molecular, Crosses, Genetic, Epidermal Growth Factor, Mice, Inbred BALB C, Mice, Neurologic Mutants, Molecular Sequence Data, Nerve Tissue Proteins, Neurons, Organ Specificity, Serine Endopeptidases, Cell Adhesion Molecules, Neuronal, Extracellular Matrix Proteins, Conserved Sequence, Gene Deletion, Sequence Deletion, In Situ Hybridization, DNA, Complementary, Gene Expression Regulation, Developmental, Mice
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