The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
Randomization of genes by PCR mutagenesis.
Open accessAbstract
A modified polymerase chain reaction (PCR) was developed to introduce random point mutations into cloned genes. The modifications were made to decrease the fidelity of Taq polymerase during DNA synthesis without significantly decreasing the level of amplification achieved in the PCR. The resulting PCR products can be cloned to produce random mutant libraries or transcribed directly if a T7 promoter is incorporated within the appropriate PCR primer. We used this method to mutagenize the gene that encodes the Tetrahymena ribozyme with a mutation rate of 0.66% +/- 0.13% (95% C.I.) per position per PCR, as determined by sequence analysis. There are no strong preferneces with respect to the type of base substituion. The number of mutations per DNA sequence follows a Poisson distribution and the mutations are randomly distributed throughout the amplified sequence.
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Cites
Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA Polymerase
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Isolation of New Ribozymes from a Large Pool of Random Sequences
Bartel, D. P., & Szostak, J. W. (1993) · Science · doi:10.1126/science.7690155 · Cited by 900
Directed Evolution of Nucleic Acid Enzymes
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Self-citationZaccolo, M., & Gherardi, E. (1999) · Journal of Molecular Biology · doi:10.1006/jmbi.1998.2262 · Cited by 200
A cross-chiral RNA polymerase ribozyme
Sczepanski, J. T., & Joyce, G. F. (2014) · Nature · doi:10.1038/nature13900 · Cited by 186
Self-citationHOWE, K. J., KANE, C. M., & ARES, M. (2003) · RNA · doi:10.1261/rna.5390803 · Cited by 169
A Selective Transport Route from Golgi to Late Endosomes That Requires the Yeast Gga Proteins
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A ribozyme that lacks cytidine
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RNA-Catalyzed Cross-Chiral Polymerization of RNA
Tjhung, K. F., Sczepanski, J. T., Murtfeldt, E. R., & Joyce, G. F. (2020) · Journal of the American Chemical Society · doi:10.1021/jacs.0c05635 · Cited by 29
Self-citationDistribution and molecular evolution of the anti-CRISPR family AcrIF7
Figueroa, W., Cazares, A., Cazares, D., Wu, Y., de la Cruz, A., Welch, M., Kameyama, L., Nobrega, F. L., & Guarneros, G. (2023) · PLOS Biology · doi:10.1371/journal.pbio.3002072 · Cited by 2
Metadata
- Authors
- Cadwell, R. C., & Joyce, G. F.
- Venue
- Genome Research
- Publisher
- Cold Spring Harbor Laboratory
- Published
- 1992-08
- Volume
- 2
- Issue
- 1
- Pages
- 28-33
- ISSN
- 1088-9051 (ISSN-L)
- OpenAlex
- W2135636849
- Cited by
- 1,068
- References
- 2
- Type
- posted content
- Language
- en
- Open access
- bronze
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