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Cited by 326

The DOI matches its Crossref record; 46 of the 46 references it cites were found in a registry or an index.

Decoding Randomly Ordered DNA Arrays

Open access
Gunderson, Kevin L., Kruglyak, Semyon, Graige, Michael S., Garcia, Francisco, Kermani, Bahram G., Zhao, Chanfeng, Che, Diping, Dickinson, Todd, Wickham, Eliza, Bierle, Jim, Doucet, Dennis, Milewski, Monika, Yang, Robert, Siegmund, Chris, Haas, Juergen, Zhou, Lixin, Oliphant, Arnold, Fan, Jian-Bing, Barnard, Steven, Chee, Mark S.·2004·Genome Research

Abstract

We have developed a simple and efficient algorithm to identify each member of a large collection of DNA-linked objects through the use of hybridization, and have applied it to the manufacture of randomly assembled arrays of beads in wells. Once the algorithm has been used to determine the identity of each bead, the microarray can be used in a wide variety of applications, including single nucleotide polymorphism genotyping and gene expression profiling. The algorithm requires only a few labels and several sequential hybridizations to identify thousands of different DNA sequences with great accuracy. We have decoded tens of thousands of arrays, each with 1520 sequences represented at ∼30-fold redundancy by up to ∼50,000 beads, with a median error rate of <1 × 10-4 per bead. The approach makes use of error checking codes and provides, for the first time, a direct functional quality control of every element of each array that is manufactured. The algorithm can be applied to any spatially fixed collection of objects or molecules that are associated with specific DNA sequences.

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Metadata

Authors
Gunderson, K. L., Kruglyak, S., Graige, M. S., Garcia, F., Kermani, B. G., Zhao, C., Che, D., Dickinson, T., Wickham, E., Bierle, J., Doucet, D., Milewski, M., Yang, R., Siegmund, C., Haas, J., Zhou, L., Oliphant, A., Fan, J.-B., Barnard, S., & Chee, M. S.
Venue
Genome Research
Published
2004-04-12
Volume
14
Issue
5
Pages
870-877
ISSN
1088-9051 (ISSN-L)
OpenAlex
W2109178214
Cited by
326
References
46
Type
posted content
Language
en
Open access
bronze

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CoChat. (2026). Citation check: Decoding Randomly Ordered DNA Arrays. https://app.cochat.ai/papers/10.1101/gr.2255804
The paper
Gunderson, K. L., Kruglyak, S., Graige, M. S., Garcia, F., Kermani, B. G., Zhao, C., Che, D., Dickinson, T., Wickham, E., Bierle, J., Doucet, D., Milewski, M., Yang, R., Siegmund, C., Haas, J., Zhou, L., Oliphant, A., Fan, J.-B., Barnard, S., & Chee, M. S. (2004). Decoding Randomly Ordered DNA Arrays. Genome Research. https://doi.org/10.1101/gr.2255804

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