The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
Versatile Gene-Specific Sequence Tags for Arabidopsis Functional Genomics: Transcript Profiling and Reverse Genetics Applications
Open accessAbstract
Microarray transcript profiling and RNA interference are two new technologies crucial for large-scale gene function studies in multicellular eukaryotes. Both rely on sequence-specific hybridization between complementary nucleic acid strands, inciting us to create a collection of gene-specific sequence tags (GSTs) representing at least 21,500 Arabidopsis genes and which are compatible with both approaches. The GSTs were carefully selected to ensure that each of them shared no significant similarity with any other region in the Arabidopsis genome. They were synthesized by PCR amplification from genomic DNA. Spotted microarrays fabricated from the GSTs show good dynamic range, specificity, and sensitivity in transcript profiling experiments. The GSTs have also been transferred to bacterial plasmid vectors via recombinational cloning protocols. These cloned GSTs constitute the ideal starting point for a variety of functional approaches, including reverse genetics. We have subcloned GSTs on a large scale into vectors designed for gene silencing in plant cells. We show that in planta expression of GST hairpin RNA results in the expected phenotypes in silenced Arabidopsis lines. These versatile GST resources provide novel and powerful tools for functional genomics.
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Cites
Analysis of the genome sequence of the flowering plant Arabidopsis thaliana
Initiative, T. A. G. (2000) · Nature · doi:10.1038/35048692 · Cited by 9,301
TM4: a free, open-source system for microarray data management and analysis
Saeed, A. I., Sharov, V., White, J., Li, J., Liang, W., Bhagabati, N., Braisted, J., Klapa, M., Currier, T., Thiagarajan, M., et al. (2003) · BioTechniques · doi:10.2144/03342mt01 · Cited by 4,837
Auxin-responsive gene expression: genes, promoters and regulatory factors
Hagen, G., & Guilfoyle, T. (2002) · Plant Molecular Biology · doi:10.1023/a:1015207114117 · Cited by 1,262
Cited by
Lurin, C., Andreés, C., Aubourg, S., Bellaoui, M., Bitton, F., Bruyère, C., Caboche, M., Debast, C., Gualberto, J., Hoffmann, B., et al. (2004) · The Plant Cell · doi:10.1105/tpc.104.022236 · Cited by 1,306
Self-citationNINJA connects the co-repressor TOPLESS to jasmonate signalling
Pauwels, L., Barbero, G. F., Geerinck, J., Tilleman, S., Grunewald, W., Pérez, A. C., Chico, J. M., Bossche, R. V., Sewell, J., Gil, E., et al. (2010) · Nature · doi:10.1038/nature08854 · Cited by 1,018
Gene silencing in plants using artificial microRNAs and other small RNAs
Ossowski, S., Schwab, R., & Weigel, D. (2008) · The Plant Journal · doi:10.1111/j.1365-313x.2007.03328.x · Cited by 685
Berthet, S., Demont-Caulet, N., Pollet, B., Bidzinski, P., Cézard, L., Le Bris, P., Borrega, N., Hervé, J., Blondet, E., Balzergue, S., et al. (2011) · The Plant Cell · doi:10.1105/tpc.110.082792 · Cited by 601
Achard, P., Renou, J.-P., Berthomé, R., Harberd, N. P., & Genschik, P. (2008) · Current Biology · doi:10.1016/j.cub.2008.04.034 · Cited by 577
Metadata
- Authors
- Hilson, P., Allemeersch, J., Altmann, T., Aubourg, S., Avon, A., Beynon, J., Bhalerao, R. P., Bitton, F., Caboche, M., Cannoot, B., Chardakov, V., Cognet-Holliger, C., Colot, V., Crowe, M., Darimont, C., Durinck, S., Eickhoff, H., de Longevialle, A. F., Farmer, E. E., Grant, M., Kuiper, M. T. R., Lehrach, H., Léon, C., Leyva, A., Lundeberg, J., Lurin, C., Moreau, Y., Nietfeld, W., Paz-Ares, J., Reymond, P., Rouzé, P., Sandberg, G., Segura, M. D., Serizet, C., Tabrett, A., Taconnat, L., Thareau, V., Van Hummelen, P., Vercruysse, S., Vuylsteke, M., Weingartner, M., Weisbeek, P. J., Wirta, V., Wittink, F. R. A., Zabeau, M., & Small, I.
- Venue
- Genome Research
- Publisher
- Cold Spring Harbor Laboratory
- Published
- 2004-10-15
- Volume
- 14
- Issue
- 10b
- Pages
- 2176-2189
- ISSN
- 1088-9051 (ISSN-L)
- OpenAlex
- W2153236054
- Cited by
- 307
- References
- 3
- Type
- posted content
- Language
- en
- Open access
- bronze
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- Hilson, P., Allemeersch, J., Altmann, T., Aubourg, S., Avon, A., Beynon, J., Bhalerao, R. P., Bitton, F., Caboche, M., Cannoot, B., Chardakov, V., Cognet-Holliger, C., Colot, V., Crowe, M., Darimont, C., Durinck, S., Eickhoff, H., de Longevialle, A. F., Farmer, E. E., . . . Small, I. (2004). Versatile Gene-Specific Sequence Tags for Arabidopsis Functional Genomics: Transcript Profiling and Reverse Genetics Applications. Genome Research. https://doi.org/10.1101/gr.2544504
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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