Molecular Characterization of a Chromosomal Rearrangement Involved in the Adaptive Evolution of Yeast Strains
Open accessAbstract
Wine yeast strains show a high level of chromosome length polymorphism. This polymorphism is mainly generated by illegitimate recombination mediated by Ty transposons or subtelomeric repeated sequences. We have found, however, that the SSU1-R allele, which confers sulfite resistance to yeast cells, is the product of a reciprocal translocation between chromosomes VIII and XVI due to unequal crossing-over mediated by microhomology between very short sequences on the 5′ upstream regions of the SSU1 and ECM34 genes. We also show that this translocation is only present in wine yeast strains, suggesting that the use for millennia of sulfite as a preservative in wine production could have favored its selection. This is the first time that a gross chromosomal rearrangement is shown to be involved in the adaptive evolution of Saccharomyces cerevisiae. [The sequence data from this study have been submitted to EMBL under accession nos. AF239757,AF239758, and AJ458340–AJ458367. The following individual kindly provided reagents, samples, or unpublished information as indicated in the paper: N. Goto-Yamamoto.]
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The role of horizontal gene transfer in natural evolution remains a controversial topic.
“Nevertheless, the role of HGT in natural evolution remains a controversial topic [133].”
From Adaptive laboratory evolution—Principles and applications for biotechnology (2013) · cites this paper as [133]
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- Authors
- Pérez-Ortı́n, J. E., Querol, A., Puig, S., & Barrio, E.
- Affiliations
- Consejo Superior de Investigaciones Científicas (ES)
- Venue
- Genome Research
- Publisher
- Cold Spring Harbor Laboratory
- Published
- 2002-10-01
- Volume
- 12
- Issue
- 10
- Pages
- 1533-1539
- ISSN
- 1088-9051 (ISSN-L)
- 1549-5469
- PMC
- PMC187534
- OpenAlex
- W2037448910
- Cited by
- 272
- References
- 28
- Citations by year
- 2026: 7 · 2025: 8 · 2024: 7 · 2023: 7 · 2022: 8 · 2021: 20 · 2020: 12 · 2019: 16 · 2018: 10 · 2017: 24 · 2016: 17 · 2015: 14 · 2014: 11 · 2013: 15 · 2012: 11
- Type
- posted content
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Genome Research (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- LA Referencia (Red Federada de Repositorios Institucionales de Publicaciones Científicas) (submittedVersion, other-oa)
- Repository of Digital Objects for Teaching Research and Culture (University of Valencia) (submittedVersion, cc-by-nc)
- Funders
- European Commission (BIO4-CT97-2294)
- Generalitat Valenciana
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Chromosomal and Genetic Variations
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Yeasts and Rust Fungi Studies
- Keywords
- chromosomal rearrangements, reciprocal translocation, adaptive evolution, Saccharomyces cerevisiae, wine yeast, unequal crossover, microhomology, chromosome length polymorphism, subtelomeric repeats, wine preservatives
- MeSH terms
- Base Sequence, DNA, Fungal, Gene Frequency, Genes, Fungal, Genetic Markers, Molecular Sequence Data, Recombination, Genetic, Saccharomyces cerevisiae, Species Specificity, Translocation, Genetic, Gene Rearrangement, Chromosomes, Fungal, Genome, Fungal, Evolution, Molecular, Anion Transport Proteins, Saccharomyces cerevisiae Proteins
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