Human Whole-Genome Shotgun Sequencing
Open accessAbstract
Large-scale sequencing of the human genome is now under way (Boguski et al. 1996;Marshall and Pennisi 1996).Although at the beginning of the Genome Project, many doubted the scientific value of sequencing the entire human genome, these doubts have evaporated almost entirely (Gibbs 1995;Olson 1995).Primary reasons for generating the human genomic sequence are listed in Table 1.The approach being taken for human genomic sequencing is the same as that used for the Saccharomyces cerevisiae and Caenorhabditis elegans genomes, namely construction of overlapping arrays of large insert Escherichia coli clones, followed by complete sequencing of these clones one at a time.In this article, we outline an alternative approach to sequencing the human and other large genomes, which we argue is less costly and more informative than the clone-by-clone approach.
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Nematostella genes are more similar to vertebrate genes than to fly and nematode genes, based on Bayesian branch length estimation and percent sequence identity.
“We compared predicted protein sequences from Nematostella to those from other metazoan and outgroup genomes, and find that Nematostella genes are more similar to vertebrate genes than to fly and nematode genes using bayesian branch length estimation and an analysis of percent sequence identity.”
From Sea Anemone Genome Reveals Ancestral Eumetazoan Gene Repertoire and Genomic Organization (2007) · cites this paper as [31]
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- Authors
- Weber, J. L., & Myers, E. W.
- Affiliations
- University of Arizona (US)
- ORCID
- Myers, Eugene W.
- Venue
- Genome Research
- Publisher
- Cold Spring Harbor Laboratory
- Published
- 1997-05-01
- Volume
- 7
- Issue
- 5
- Pages
- 401-409
- ISSN
- 1088-9051 (ISSN-L)
- 1549-5469
- OpenAlex
- W2125349979
- Cited by
- 311
- References
- 67
- Citations by year
- 2026: 2 · 2025: 4 · 2024: 4 · 2023: 12 · 2022: 7 · 2021: 9 · 2020: 5 · 2019: 5 · 2018: 5 · 2017: 6 · 2016: 8 · 2015: 7 · 2014: 7 · 2013: 14 · 2012: 7
- Type
- posted content
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Genome Research (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Phylogenetic Studies
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genomic variations and chromosomal abnormalities
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genome Rearrangement Algorithms
- Keywords
- human genome sequencing, whole genome shotgun sequencing, sequencing cost, Human Genome Project
- MeSH terms
- Cloning, Molecular, Forecasting, Humans, Polymorphism, Genetic, Genome, Human, Human Genome Project, Sequence Tagged Sites, Sequence Analysis, DNA
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