Cited by 222Genomics and Phylogenetic StudiesChromosomal and Genetic VariationsFractal and DNA sequence analysis

The Phusion Assembler

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Mullikin, James C., Ning, Zemin·2003·Genome Research

Abstract

The Phusion assembler has assembled the mouse genome from the whole-genome shotgun (WGS) dataset collected by the Mouse Genome Sequencing Consortium, at ∼7.5× sequence coverage, producing a high-quality draft assembly 2.6 gigabases in size, of which 90% of these bases are in 479 scaffolds. For the mouse genome, which is a large and repeat-rich genome, the input dataset was designed to include a high proportion of paired end sequences of various size selected inserts, from 2–200 kbp lengths, into various host vector templates. Phusion uses sequence data, called reads, and information about reads that share common templates, called read pairs, to drive the assembly of this large genome to highly accurate results. The preassembly stage, which clusters the reads into sensible groups, is a key element of the entire assembler, because it permits a simple approach to parallelization of the assembly stage, as each cluster can be treated independent of the others. In addition to the application of Phusion to the mouse genome, we will also present results from the WGS assembly of Caenorhabditis briggsae sequenced to about 11× coverage. The C. briggsae assembly was accessioned through EMBL,http://www.ebi.ac.uk/services/index.html, using the series CAAC01000001–CAAC01000578, however, the Phusion mouse assembly described here was not accessioned. The mouse data was generated by the Mouse Genome Sequencing Consortium. The C. briggsae sequence was generated at The Wellcome Trust Sanger Institute and the Genome Sequencing Center, Washington University School of Medicine.

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Research works printed but not linked: 1

  1. Phusion C.briggsae assembly. ftp://ftp.tigr.org/pub/data/m_musculus/bac_end_sequences/mbends; Mouse BACend sequence. http://genome.cse.ucsc.edu/cgi-bin/hgGateway?db=mm1; Phusion assembly. http://genome.wustl.edu/projects/cbriggsae/index.php; Caenorhabditis Briggsae. http://genome.wustl.edu/projects/mouse/index.php?fpc=1; Mouse Genome. http://trace.ensembl.org/; Ensembl Genome Server. ftp://wolfram.wi.mit.edu/pub/mouse_contigs/; ARACHNE mouse assembly. http://www.ebi.ac.uk/services/index.html; EBI Services. http://www.ncbi.nlm.nih.gov/Traces/trace.cgi?; Trace DB. http://www.phrap.org/; Genome Software Development Page.

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Authors
Mullikin, J. C., & Ning, Z.
Affiliations
Wellcome Sanger Institute (GB)
Venue
Genome Research
Published
2002-12-30
Volume
13
Issue
1
Pages
81-90
ISSN
  • 1088-9051 (ISSN-L)
  • 1549-5469
OpenAlex
W2161668137
Cited by
222
References
21
Citations by year
2025: 1 · 2024: 4 · 2022: 2 · 2021: 4 · 2020: 6 · 2019: 3 · 2018: 7 · 2017: 2 · 2016: 14 · 2015: 11 · 2014: 11 · 2013: 11 · 2012: 11
Type
posted content
Language
en
Open access
Locations
  • Genome Research (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
Wellcome Trust
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Phylogenetic Studies
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Chromosomal and Genetic Variations
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fractal and DNA sequence analysis
Keywords
genome assembly, mouse genome, whole genome shotgun sequencing, paired-end reads, read clustering, parallel assembly, Caenorhabditis briggsae, draft genome sequence
MeSH terms
Animals, Software, Genome, Caenorhabditis elegans, Computational Biology, Contig Mapping, Mice

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