The Phusion Assembler
Open accessAbstract
The Phusion assembler has assembled the mouse genome from the whole-genome shotgun (WGS) dataset collected by the Mouse Genome Sequencing Consortium, at ∼7.5× sequence coverage, producing a high-quality draft assembly 2.6 gigabases in size, of which 90% of these bases are in 479 scaffolds. For the mouse genome, which is a large and repeat-rich genome, the input dataset was designed to include a high proportion of paired end sequences of various size selected inserts, from 2–200 kbp lengths, into various host vector templates. Phusion uses sequence data, called reads, and information about reads that share common templates, called read pairs, to drive the assembly of this large genome to highly accurate results. The preassembly stage, which clusters the reads into sensible groups, is a key element of the entire assembler, because it permits a simple approach to parallelization of the assembly stage, as each cluster can be treated independent of the others. In addition to the application of Phusion to the mouse genome, we will also present results from the WGS assembly of Caenorhabditis briggsae sequenced to about 11× coverage. The C. briggsae assembly was accessioned through EMBL,http://www.ebi.ac.uk/services/index.html, using the series CAAC01000001–CAAC01000578, however, the Phusion mouse assembly described here was not accessioned. The mouse data was generated by the Mouse Genome Sequencing Consortium. The C. briggsae sequence was generated at The Wellcome Trust Sanger Institute and the Genome Sequencing Center, Washington University School of Medicine.
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Research works printed but not linked: 1
- Phusion C.briggsae assembly. ftp://ftp.tigr.org/pub/data/m_musculus/bac_end_sequences/mbends; Mouse BACend sequence. http://genome.cse.ucsc.edu/cgi-bin/hgGateway?db=mm1; Phusion assembly. http://genome.wustl.edu/projects/cbriggsae/index.php; Caenorhabditis Briggsae. http://genome.wustl.edu/projects/mouse/index.php?fpc=1; Mouse Genome. http://trace.ensembl.org/; Ensembl Genome Server. ftp://wolfram.wi.mit.edu/pub/mouse_contigs/; ARACHNE mouse assembly. http://www.ebi.ac.uk/services/index.html; EBI Services. http://www.ncbi.nlm.nih.gov/Traces/trace.cgi?; Trace DB. http://www.phrap.org/; Genome Software Development Page.
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Light whole genome sequence for SNP discovery across domestic cat breeds
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A de novo metagenomic assembly program for shotgun DNA reads
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Crystallizing short-read assemblies around seeds
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- Authors
- Mullikin, J. C., & Ning, Z.
- Affiliations
- Wellcome Sanger Institute (GB)
- Venue
- Genome Research
- Publisher
- Cold Spring Harbor Laboratory
- Published
- 2002-12-30
- Volume
- 13
- Issue
- 1
- Pages
- 81-90
- ISSN
- 1088-9051 (ISSN-L)
- 1549-5469
- PMC
- PMC430959
- OpenAlex
- W2161668137
- Cited by
- 222
- References
- 21
- Citations by year
- 2025: 1 · 2024: 4 · 2022: 2 · 2021: 4 · 2020: 6 · 2019: 3 · 2018: 7 · 2017: 2 · 2016: 14 · 2015: 11 · 2014: 11 · 2013: 11 · 2012: 11
- Type
- posted content
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Genome Research (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Wellcome Trust
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Phylogenetic Studies
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Chromosomal and Genetic Variations
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fractal and DNA sequence analysis
- Keywords
- genome assembly, mouse genome, whole genome shotgun sequencing, paired-end reads, read clustering, parallel assembly, Caenorhabditis briggsae, draft genome sequence
- MeSH terms
- Animals, Software, Genome, Caenorhabditis elegans, Computational Biology, Contig Mapping, Mice
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