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An STS-Based Radiation Hybrid Map of the Human Genome

Open access
Stewart, Elizabeth A., McKusick, Kathleen B., Aggarwal, Amita, Bajorek, Ewa, Brady, Shannon, Chu, Angela, Fang, Nicole, Hadley, David, Harris, Mark, Hussain, Sami, Lee, Richard, Maratukulam, Annu, O’Connor, Kyle, Perkins, Shanti, Piercy, Mark, Qin, Fawn, Reif, Tim, Sanders, Carla, She, Xiaohong, Sun, Wei-Lin, Tabar, Poroshat, Voyticky, Susan, Cowles, Sid, Fan, Jian-Bing, Mader, Chris, Quackenbush, John, Myers, Richard M., Cox, David R.·1997·Genome Research

Abstract

We have constructed a physical map of the human genome by using a panel of 83 whole genome radiation hybrids (the Stanford G3 panel) in conjunction with 10,478 sequence-tagged sites (STSs) derived from random genomic DNA sequences, previously mapped genetic markers, and expressed sequences. Of these STSs, 5049 are framework markers that fall into 1766 high-confidence bins. An additional 945 STSs are indistinguishable in their map location from one or more of the framework markers. These 5994 mapped STSs have an average spacing of 500 kb. An additional 4484 STSs are positioned with respect to the framework markers. Comparison of the orders of markers on this map with orders derived from independent meiotic and YAC STS-content maps indicates that the error rate in defining high-confidence bins is <5%. Analysis of 322 random cDNAs indicates that the map covers the vast majority of the human genome. This STS-based radiation hybrid map of the human genome brings us one step closer to the goal of a physical map containing 30,000 unique ordered landmarks with an average marker spacing of 100 kb.

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Metadata

Authors
Stewart, E. A., McKusick, K. B., Aggarwal, A., Bajorek, E., Brady, S., Chu, A., Fang, N., Hadley, D., Harris, M., Hussain, S., Lee, R., Maratukulam, A., O’Connor, K., Perkins, S., Piercy, M., Qin, F., Reif, T., Sanders, C., She, X., Sun, W.-L., Tabar, P., Voyticky, S., Cowles, S., Fan, J.-B., Mader, C., Quackenbush, J., Myers, R. M., & Cox, D. R.
Venue
Genome Research
Published
1997-05-01
Volume
7
Issue
5
Pages
422-433
ISSN
  • 1088-9051 (ISSN-L)
  • 1549-5469
OpenAlex
W2130924644
Cited by
313
References
23
Type
posted content
Language
en
Open access
bronze

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CoChat. (2026). Citation check: An STS-Based Radiation Hybrid Map of the Human Genome. https://app.cochat.ai/papers/10.1101/gr.7.5.422
The paper
Stewart, E. A., McKusick, K. B., Aggarwal, A., Bajorek, E., Brady, S., Chu, A., Fang, N., Hadley, D., Harris, M., Hussain, S., Lee, R., Maratukulam, A., O’Connor, K., Perkins, S., Piercy, M., Qin, F., Reif, T., Sanders, C., She, X., . . . Cox, D. R. (1997). An STS-Based Radiation Hybrid Map of the Human Genome. Genome Research. https://doi.org/10.1101/gr.7.5.422

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