The DOI matches its Crossref record; 33 of the 33 references it cites were found in a registry or an index.
Optimal Point Spread Function Design for 3D Imaging
Open accessAbstract
To extract from an image of a single nanoscale object maximum physical information about its position, we propose and demonstrate a framework for pupil-plane modulation for 3D imaging applications requiring precise localization, including single-particle tracking and superresolution microscopy. The method is based on maximizing the information content of the system, by formulating and solving the appropriate optimization problem--finding the pupil-plane phase pattern that would yield a point spread function (PSF) with optimal Fisher information properties. We use our method to generate and experimentally demonstrate two example PSFs: one optimized for 3D localization precision over a 3 μm depth of field, and another with an unprecedented 5 μm depth of field, both designed to perform under physically common conditions of high background signals.
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Cites
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Fluorescence nanoscopy in cell biology
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Single-molecule spectroscopy and imaging over the decades
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Metadata
- Authors
- Shechtman, Y., Sahl, S. J., Backer, A. S., & Moerner, W. E.
- Venue
- Physical Review Letters
- Publisher
- American Physical Society (APS)
- Published
- 2014-09-26
- Volume
- 113
- Issue
- 13
- Pages
- 133902-133902
- ISSN
- 0031-9007 (ISSN-L)
- 1079-7114
- OpenAlex
- W2330285287
- Cited by
- 384
- References
- 33
- FWCI
- 9.69
- Type
- journal article
- Language
- en
- Open access
- green
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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