The DOI matches its Crossref record; 21 of the 21 references it cites were found in a registry or an index.
Agrobacterium tumefaciens -Mediated Transformation of Maize Embryos Using a Standard Binary Vector System
Abstract
We have achieved routine transformation of maize (Zea mays) using an Agrobacterium tumefaciensstandard binary (non-super binary) vector system. Immature zygotic embryos of the hybrid line Hi II were infected with A. tumefaciens strain EHA101 harboring a standard binary vector and cocultivated in the presence of 400 mg L−1 l-cysteine. Inclusion of l-cysteine in cocultivation medium lead to an improvement in transient β-glucuronidase expression observed in targeted cells and a significant increase in stable transformation efficiency, but was associated with a decrease in embryo response after cocultivation. The average stable transformation efficiency (no. of bialaphos-resistant events recovered per 100 embryos infected) of the present protocol was 5.5%. Southern-blot and progeny analyses confirmed the integration, expression, and inheritance of the bar andgus transgenes in R0, R1, and R2 generations of transgenic events. To our knowledge, this represents the first report in which fertile, stable transgenic maize has been routinely produced using an A. tumefaciensstandard binary vector system.
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Cites
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Agrobacterium -Mediated Plant Transformation: the Biology behind the “Gene-Jockeying” Tool
Gelvin, S. B. (2003) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.67.1.16-37.2003 · Cited by 1,379
Precise genome modification in the crop species Zea mays using zinc-finger nucleases
Shukla, V. K., Doyon, Y., Miller, J. C., DeKelver, R. C., Moehle, E. A., Worden, S. E., Mitchell, J. C., Arnold, N. L., Gopalan, S., Meng, X., et al. (2009) · Nature · doi:10.1038/nature07992 · Cited by 958
Morphogenic Regulators Baby boom and Wuschel Improve Monocot Transformation
Lowe, K., Wu, E., Wang, N., Hoerster, G., Hastings, C., Cho, M.-J., Scelonge, C., Lenderts, B., Chamberlin, M., Cushatt, J., et al. (2016) · The Plant Cell · doi:10.1105/tpc.16.00124 · Cited by 881
Agrobacterium-mediated transformation of maize
Ishida, Y., Hiei, Y., & Komari, T. (2007) · Nature Protocols · doi:10.1038/nprot.2007.241 · Cited by 295
Frame, B., Warnberg, K., Main, M., & Wang, K. (2015) · Methods in Molecular Biology · doi:10.1007/978-1-4939-1695-5_8 · Cited by 266
Self-citationExpression of the Nicotiana protein kinase (NPK1) enhanced drought tolerance in transgenic maize.
Shou, H. (2004) · Journal of Experimental Botany · doi:10.1093/jxb/erh129 · Cited by 250
Self-citationMookkan, M., Nelson-Vasilchik, K., Hague, J., Zhang, Z. J., & Kausch, A. P. (2017) · Plant Cell Reports · doi:10.1007/s00299-017-2169-1 · Cited by 162
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Metadata
- Authors
- Frame, B. R., Shou, H., Chikwamba, R. K., Zhang, Z., Xiang, C., Fonger, T. M., Pegg, S. E. K., Li, B., Nettleton, D. S., Pei, D., & Wang, K.
- Venue
- Plant Physiology
- Publisher
- Oxford University Press (OUP)
- Published
- 2002-05-01
- Volume
- 129
- Issue
- 1
- Pages
- 13-22
- ISSN
- 0032-0889 (ISSN-L)
- 1532-2548
- OpenAlex
- W2138079061
- Cited by
- 477
- References
- 21
- FWCI
- 4.75
- Type
- journal article
- Language
- en
- Open access
- closed
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