The DOI matches its Crossref record; 4 of the 4 references it cites were found in a registry or an index.
High-Throughput Fluorescent Tagging of Full-Length Arabidopsis Gene Products in Planta
Open accessAbstract
We developed a high-throughput methodology, termed fluorescent tagging of full-length proteins (FTFLP), to analyze expression patterns and subcellular localization of Arabidopsis gene products in planta. Determination of these parameters is a logical first step in functional characterization of the approximately one-third of all known Arabidopsis genes that encode novel proteins of unknown function. Our FTFLP-based approach offers two significant advantages: first, it produces internally-tagged full-length proteins that are likely to exhibit native intracellular localization, and second, it yields information about the tissue specificity of gene expression by the use of native promoters. To demonstrate how FTFLP may be used for characterization of the Arabidopsis proteome, we tagged a series of known proteins with diverse subcellular targeting patterns as well as several proteins with unknown function and unassigned subcellular localization.
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Cites
Analysis of the genome sequence of the flowering plant Arabidopsis thaliana
Initiative, T. A. G. (2000) · Nature · doi:10.1038/35048692 · Cited by 9,301
Global analysis of protein localization in budding yeast
Huh, W.-K., Falvo, J. V., Gerke, L. C., Carroll, A. S., Howson, R. W., Weissman, J. S., & O'Shea, E. K. (2003) · Nature · doi:10.1038/nature02026 · Cited by 4,248
Latrunculins: Novel Marine Toxins That Disrupt Microfilament Organization in Cultured Cells
Spector, I., Shochet, N. R., Kashman, Y., & Groweiss, A. (1983) · Science · doi:10.1126/science.6681676 · Cited by 750
CHLOROPLAST UNUSUAL POSITIONING1 Is Essential for Proper Chloroplast Positioning
Oikawa, K., Kasahara, M., Kiyosue, T., Kagawa, T., Suetsugu, N., Takahashi, F., Kanegae, T., Niwa, Y., Kadota, A., & Wada, M. (2003) · The Plant Cell · doi:10.1105/tpc.016428 · Cited by 258
Cited by
Nelson, B. K., Cai, X., & Nebenführ, A. (2007) · The Plant Journal · doi:10.1111/j.1365-313x.2007.03212.x · Cited by 2,083
Cell-specific nitrogen responses mediate developmental plasticity
Gifford, M. L., Dean, A., Gutierrez, R. A., Coruzzi, G. M., & Birnbaum, K. D. (2008) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0709559105 · Cited by 615
Unraveling abiotic stress tolerance mechanisms – getting genomics going
Bohnert, H. J., Gong, Q., Li, P., & Ma, S. (2006) · Current Opinion in Plant Biology · doi:10.1016/j.pbi.2006.01.003 · Cited by 434
SUBA: the Arabidopsis Subcellular Database
Heazlewood, J. L., Verboom, R. E., Tonti-Filippini, J., Small, I., & Millar, A. H. (2007) · Nucleic Acids Research · doi:10.1093/nar/gkl863 · Cited by 428
Specific Targeting of a Plasmodesmal Protein Affecting Cell-to-Cell Communication
Thomas, C. L., Bayer, E. M., Ritzenthaler, C., Fernandez-Calvino, L., & Maule, A. J. (2008) · PLoS Biology · doi:10.1371/journal.pbio.0060007 · Cited by 358
Metadata
- Authors
- Tian, G.-W., Mohanty, A., Chary, S. N., Li, S., Paap, B., Drakakaki, G., Kopec, C. D., Li, J., Ehrhardt, D., Jackson, D., Rhee, S. Y., Raikhel, N. V., & Citovsky, V.
- Venue
- Plant Physiology
- Publisher
- Oxford University Press (OUP)
- Published
- 2004-05-01
- Volume
- 135
- Issue
- 1
- Pages
- 25-38
- ISSN
- 0032-0889 (ISSN-L)
- 1532-2548
- OpenAlex
- W2121639904
- Cited by
- 236
- References
- 4
- FWCI
- 20.62
- Type
- journal article
- Language
- en
- Open access
- green
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- Tian, G.-W., Mohanty, A., Chary, S. N., Li, S., Paap, B., Drakakaki, G., Kopec, C. D., Li, J., Ehrhardt, D., Jackson, D., Rhee, S. Y., Raikhel, N. V., & Citovsky, V. (2004). High-Throughput Fluorescent Tagging of Full-Length Arabidopsis Gene Products in Planta. Plant Physiology. https://doi.org/10.1104/pp.104.040139
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