Cited by 91Photosynthetic Processes and MechanismsAlgal biology and biofuel productionMicrobial Metabolic Engineering and Bioproduction

Metabolome Phenotyping of Inorganic Carbon Limitation in Cells of the Wild Type and Photorespiratory Mutants of the Cyanobacterium Synechocystis sp. Strain PCC 6803

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Eisenhut, Marion, Huege, Jan, Schwarz, Doreen, Bauwe, Hermann, Kopka, Joachim, Hagemann, Martin·2008·Plant Physiology

Abstract

The amount of inorganic carbon represents one of the main environmental factors determining productivity of photoautotrophic organisms. Using the model cyanobacterium Synechocystis sp. PCC 6803, we performed a first metabolome study with cyanobacterial cells shifted from high CO2 (5% in air) into conditions of low CO2 (LC; ambient air with 0.035% CO2). Using gas chromatography-mass spectrometry, 74 metabolites were reproducibly identified under different growth conditions. Shifting wild-type cells into LC conditions resulted in a global metabolic reprogramming and involved increases of, for example, 2-oxoglutarate (2OG) and phosphoenolpyruvate, and reductions of, for example, sucrose and fructose-1,6-bisphosphate. A decrease in Calvin-Benson cycle activity and increased usage of associated carbon cycling routes, including photorespiratory metabolism, was indicated by synergistic accumulation of the fumarate, malate, and 2-phosphoglycolate pools and a transient increase of 3-phosphoglycerate. The unexpected accumulation of 2OG with a concomitant decrease of glutamine pointed toward reduced nitrogen availability when cells are confronted with LC. Despite the increase in 2OG and low amino acid pools, we found a complete dephosphorylation of the PII regulatory protein at LC characteristic for nitrogen-replete conditions. Moreover, mutants with defined blocks in the photorespiratory metabolism leading to the accumulation of glycolate and glycine, respectively, exhibited features of LC-treated wild-type cells such as the changed 2OG to glutamine ratio and PII phosphorylation state already under high CO2 conditions. Thus, metabolome profiling demonstrated that acclimation to LC involves coordinated changes of carbon and interacting nitrogen metabolism. We hypothesize that Synechocystis has a temporal lag of acclimating carbon versus nitrogen metabolism with carbon leading.

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  1. Erban A, Schauer N, Fernie AR, Kopka J (2007) Non-supervised construction and application of mass spectral and retention time index libraries from time-of-flight GC-MS metabolite profiles. In W Weckwerth, ed, Metabolomics: Methods and Protocols. Humana Press, Totowa, NJ, pp 19–38 [DOI] [PubMed]
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Authors
Eisenhut, M., Huege, J., Schwarz, D., Bauwe, H., Kopka, J., & Hagemann, M.
Affiliations
Max Planck Institute of Molecular Plant Physiology (DE)
Venue
Plant Physiology
Published
2008-10-22
Volume
148
Issue
4
Pages
2109-2120
ISSN
  • 0032-0889 (ISSN-L)
  • 1532-2548
OpenAlex
W2124103764
Cited by
91
References
43
Citations by year
2026: 2 · 2025: 3 · 2024: 4 · 2023: 7 · 2021: 8 · 2020: 10 · 2019: 3 · 2018: 5 · 2017: 1 · 2016: 8 · 2015: 9 · 2014: 4 · 2013: 7 · 2012: 6
FWCI
1.92
Type
journal article
Language
en
Open access
Locations
  • PLANT PHYSIOLOGY (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
  • Max Planck Institute for Plasma Physics (submittedVersion)
  • MPG.PuRe (Max Planck Society) (submittedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Photosynthetic Processes and Mechanisms
  • Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
Keywords
Synechocystis sp. PCC 6803, inorganic carbon limitation, photorespiration, alpha-ketoglutarate, nitrogen metabolism, Calvin cycle, glycolate, glycine, metabolite profiling, carbon-nitrogen interactions
MeSH terms
Acclimatization, Bacteriological Techniques, Carbon, Carbon Dioxide, Glycolates, Mutation, Nitrogen, Phenotype, Phosphorylation, Gene Expression Profiling, Synechocystis, PII Nitrogen Regulatory Proteins, Phototrophic Processes

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