Metabolome Phenotyping of Inorganic Carbon Limitation in Cells of the Wild Type and Photorespiratory Mutants of the Cyanobacterium Synechocystis sp. Strain PCC 6803
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The amount of inorganic carbon represents one of the main environmental factors determining productivity of photoautotrophic organisms. Using the model cyanobacterium Synechocystis sp. PCC 6803, we performed a first metabolome study with cyanobacterial cells shifted from high CO2 (5% in air) into conditions of low CO2 (LC; ambient air with 0.035% CO2). Using gas chromatography-mass spectrometry, 74 metabolites were reproducibly identified under different growth conditions. Shifting wild-type cells into LC conditions resulted in a global metabolic reprogramming and involved increases of, for example, 2-oxoglutarate (2OG) and phosphoenolpyruvate, and reductions of, for example, sucrose and fructose-1,6-bisphosphate. A decrease in Calvin-Benson cycle activity and increased usage of associated carbon cycling routes, including photorespiratory metabolism, was indicated by synergistic accumulation of the fumarate, malate, and 2-phosphoglycolate pools and a transient increase of 3-phosphoglycerate. The unexpected accumulation of 2OG with a concomitant decrease of glutamine pointed toward reduced nitrogen availability when cells are confronted with LC. Despite the increase in 2OG and low amino acid pools, we found a complete dephosphorylation of the PII regulatory protein at LC characteristic for nitrogen-replete conditions. Moreover, mutants with defined blocks in the photorespiratory metabolism leading to the accumulation of glycolate and glycine, respectively, exhibited features of LC-treated wild-type cells such as the changed 2OG to glutamine ratio and PII phosphorylation state already under high CO2 conditions. Thus, metabolome profiling demonstrated that acclimation to LC involves coordinated changes of carbon and interacting nitrogen metabolism. We hypothesize that Synechocystis has a temporal lag of acclimating carbon versus nitrogen metabolism with carbon leading.
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2-oxoglutarate modulates N and C metabolism in non-nitrogen-assimilating cyanobacteria.
“In the same way, this compound is also considered to modulate the N and C metabolism in non-nitrogen-assimilating cyanobacteria (Eisenhut et al. 2008; Muro-Pastor et al. 2005).”
From Renewable energy from Cyanobacteria: energy production optimization by metabolic pathway engineering (2011) · cites this paper as Eisenhut et al. 2008
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Metabolites, Molecular Diversity, and Applications of Cyanobacteria in the Beverage Industry
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- Authors
- Eisenhut, M., Huege, J., Schwarz, D., Bauwe, H., Kopka, J., & Hagemann, M.
- Affiliations
- Max Planck Institute of Molecular Plant Physiology (DE)
- Venue
- Plant Physiology
- Publisher
- Oxford University Press (OUP)
- Published
- 2008-10-22
- Volume
- 148
- Issue
- 4
- Pages
- 2109-2120
- ISSN
- 0032-0889 (ISSN-L)
- 1532-2548
- PMC
- PMC2593672
- OpenAlex
- W2124103764
- Cited by
- 91
- References
- 43
- Citations by year
- 2026: 2 · 2025: 3 · 2024: 4 · 2023: 7 · 2021: 8 · 2020: 10 · 2019: 3 · 2018: 5 · 2017: 1 · 2016: 8 · 2015: 9 · 2014: 4 · 2013: 7 · 2012: 6
- FWCI
- 1.92
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Licence
- academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model (vor)
- Locations
- PLANT PHYSIOLOGY (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Max Planck Institute for Plasma Physics (submittedVersion)
- MPG.PuRe (Max Planck Society) (submittedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Photosynthetic Processes and Mechanisms
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Keywords
- Synechocystis sp. PCC 6803, inorganic carbon limitation, photorespiration, alpha-ketoglutarate, nitrogen metabolism, Calvin cycle, glycolate, glycine, metabolite profiling, carbon-nitrogen interactions
- MeSH terms
- Acclimatization, Bacteriological Techniques, Carbon, Carbon Dioxide, Glycolates, Mutation, Nitrogen, Phenotype, Phosphorylation, Gene Expression Profiling, Synechocystis, PII Nitrogen Regulatory Proteins, Phototrophic Processes
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