Succinate:Quinol Oxidoreductases in the Cyanobacterium Synechocystis sp. Strain PCC 6803: Presence and Function in Metabolism and Electron Transport
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The open reading frames sll 1625 and sll 0823, which have significant sequence similarity to genes coding for the FeS subunits of succinate dehydrogenase and fumarate reductase, were deleted singly and in combination in the cyanobacterium Synechocystis sp. strain PCC 6803. When the organic acid content in the Δ sll 1625 and Δ sll 0823 strains was analyzed, a 100-fold decrease in succinate and fumarate concentrations was observed relative to the wild type. A similar analysis for the Δ sll 1625 Δ sll 0823 strain revealed that 17% of the wild-type succinate levels remained, while only 1 to 2% of the wild-type fumarate levels were present. Addition of 2-oxoglutarate to the growth media of the double mutant strain prior to analysis of organic acids in cells caused succinate to accumulate. This indicates that succinate dehydrogenase activity had been blocked by the deletions and that 2-oxoglutarate can be converted to succinate in vivo in this organism, even though a traditional 2-oxoglutarate dehydrogenase is lacking. In addition, reduction of the thylakoid plastoquinone pool in darkness in the presence of KCN was up to fivefold slower in the mutants than in the wild type. Moreover, in vitro succinate dehydrogenase activity observed in wild-type membranes is absent from those isolated from the double mutant and reduced in those from the single mutants, further indicating that the sll 1625 and sll 0823 open reading frames encode subunits of succinate dehydrogenase complexes that are active in the thylakoid membrane of the cyanobacterium.
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In cyanobacteria, succinate can originate from 2-oxoglutarate using a different route.
“It seems that in cyanobacteria, succinate can originate from 2-OG using a different route (Cooley et al. 2000).”
From Renewable energy from Cyanobacteria: energy production optimization by metabolic pathway engineering (2011) · cites this paper as Cooley et al. 2000
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- Authors
- Cooley, J. W., Howitt, C. A., & Vermaas, W. F. J.
- Affiliations
- Arizona State University (US)
- Venue
- Journal of Bacteriology
- Publisher
- American Society for Microbiology
- Published
- 2000-02
- Volume
- 182
- Issue
- 3
- Pages
- 714-722
- ISSN
- 0021-9193 (ISSN-L)
- 1098-5530
- PMC
- PMC94334
- OpenAlex
- W2104263415
- Cited by
- 112
- References
- 32
- Citations by year
- 2026: 3 · 2025: 1 · 2024: 3 · 2022: 3 · 2021: 10 · 2020: 1 · 2019: 6 · 2018: 2 · 2017: 2 · 2016: 7 · 2015: 7 · 2014: 9 · 2013: 5 · 2012: 3
- FWCI
- 2.17
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Journal of Bacteriology (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Photosynthetic Processes and Mechanisms
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Metalloenzymes and iron-sulfur proteins
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial metabolism and enzyme function
- Keywords
- succinate dehydrogenase, Synechocystis sp. PCC 6803, cyanobacteria, fumarate, succinic acid, alpha-ketoglutarate, thylakoid membranes, electron transport, plastoquinone pool, deletion mutants, organic acid metabolism
- MeSH terms
- Cyanobacteria, Chlorophyll, Electron Transport, Fluorescence, Gas Chromatography-Mass Spectrometry, Oxidation-Reduction, Succinate Dehydrogenase, Transcription, Genetic, Gene Expression Regulation, Bacterial, Gene Expression Regulation, Enzymologic, Mutagenesis, Insertional
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