The DOI matches its Crossref record; 50 of the 50 references it cites were found in a registry or an index.
Wheat Vacuolar Iron Transporter TaVIT2 Transports Fe and Mn and Is Effective for Biofortification
Open accessAbstract
Increasing the intrinsic nutritional quality of crops, known as biofortification, is viewed as a sustainable approach to alleviate micronutrient deficiencies. In particular, iron deficiency anemia is a major global health issue, but the iron content of staple crops such as wheat (Triticum aestivum) is difficult to change because of genetic complexity and homeostasis mechanisms. To identify target genes for the biofortification of wheat, we functionally characterized homologs of the VACUOLAR IRON TRANSPORTER (VIT). The wheat genome contains two VIT paralogs, TaVIT1 and TaVIT2, which have different expression patterns but are both low in the endosperm. TaVIT2, but not TaVIT1, was able to rescue the growth of a yeast (Saccharomyces cerevisiae) mutant defective in vacuolar iron transport. TaVIT2 also complemented a manganese transporter mutant but not a vacuolar zinc transporter mutant. By overexpressing TaVIT2 under the control of an endosperm-specific promoter, we achieved a greater than 2-fold increase in iron in white flour fractions, exceeding minimum legal fortification levels in countries such as the United Kingdom. The antinutrient phytate was not increased and the iron in the white flour fraction was bioavailable in vitro, suggesting that food products made from the biofortified flour could contribute to improved iron nutrition. The single-gene approach impacted minimally on plant growth and also was effective in barley (Hordeum vulgare). Our results show that by enhancing vacuolar iron transport in the endosperm, this essential micronutrient accumulated in this tissue, bypassing existing homeostatic mechanisms.
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Cited by
Manganese in Plants: From Acquisition to Subcellular Allocation
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CRISPR for Crop Improvement: An Update Review
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Iron homeostasis in plants – a brief overview
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Self-citationHeavy metal transporters: Functional mechanisms, regulation, and application in phytoremediation
Yang, Z., Yang, F., Liu, J.-L., Wu, H.-T., Yang, H., Shi, Y., Liu, J., Zhang, Y.-F., Luo, Y.-R., & Chen, K.-M. (2022) · Science of The Total Environment · doi:10.1016/j.scitotenv.2021.151099 · Cited by 308
Metadata
- Authors
- Connorton, J. M., Jones, E. R., Rodríguez-Ramiro, I., Fairweather-Tait, S., Uauy, C., & Balk, J.
- Venue
- Plant Physiology
- Publisher
- Oxford University Press (OUP)
- Published
- 2017-07-06
- Volume
- 174
- Issue
- 4
- Pages
- 2434-2444
- ISSN
- 0032-0889 (ISSN-L)
- 1532-2548
- OpenAlex
- W2733583597
- Cited by
- 265
- References
- 50
- FWCI
- 17.76
- Type
- journal article
- Language
- en
- Open access
- hybrid
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