The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
Involvement of the Secretory Pathway and the Cytoskeleton in Intracellular Targeting and Tubule Assembly of Grapevine fanleaf virus Movement Protein in Tobacco BY-2 Cells
Open accessAbstract
Grapevine fanleaf virus (GFLV) is one of a large class of plant viruses whose cell-to-cell transport involves the passage of virions through tubules composed of virus-encoded movement protein (MP). The tubules are embedded within modified plasmodesmata, but the mechanism of targeting of MP to these sites is unknown. To study intracellular GFLV MP trafficking, a green fluorescent protein-MP fusion (GFP:MP) was expressed in transgenic tobacco BY-2 suspension cells under the control of an inducible promoter. We show that GFP:MP is targeted preferentially to calreticulin-labeled foci within the youngest cross walls, where it assembles into tubules. During cell division, GFP:MP colocalizes in the cell plate with KNOLLE, a cytokinesis-specific syntaxin, and both proteins are linked physically, as shown by coimmunoprecipitation of the two proteins from the same microsomal fraction. In addition, treatment with various drugs has revealed that a functional secretory pathway, but not the cytoskeleton, is required for tubule formation. However, correct GFP:MP targeting to calreticulin-labeled foci seems to be cytoskeleton dependent. Finally, biochemical analyses have revealed that at least a fraction of the MP behaves as an intrinsic membrane protein. These findings support a model in which GFP:MP would be transported to specific sites via Golgi-derived vesicles along two different pathways: a microtubule-dependent pathway in normal cells and a microfilament-dependent default pathway when microtubules are depolymerized.
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Cites
Functional rafts in cell membranes
Simons, K., & Ikonen, E. (1997) · Nature · doi:10.1038/42408 · Cited by 9,757
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Morton, W. M., Ayscough, K. R., & McLaughlin, P. J. (2000) · Nature Cell Biology · doi:10.1038/35014075 · Cited by 523
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Citovsky, V., Lee, L.-Y., Vyas, S., Glick, E., Chen, M.-H., Vainstein, A., Gafni, Y., Gelvin, S. B., & Tzfira, T. (2006) · Journal of Molecular Biology · doi:10.1016/j.jmb.2006.08.017 · Cited by 366
Specific Targeting of a Plasmodesmal Protein Affecting Cell-to-Cell Communication
Thomas, C. L., Bayer, E. M., Ritzenthaler, C., Fernandez-Calvino, L., & Maule, A. J. (2008) · PLoS Biology · doi:10.1371/journal.pbio.0060007 · Cited by 358
Self-citationEndocytosis of Cell Surface Material Mediates Cell Plate Formation during Plant Cytokinesis
Dhonukshe, P., Baluška, F., Schlicht, M., Hlavacka, A., Šamaj, J., Friml, J., & Gadella, T. W. J. (2006) · Developmental Cell · doi:10.1016/j.devcel.2005.11.015 · Cited by 258
Metadata
- Authors
- Laporte, C., Vetter, G., Loudes, A.-M., Robinson, D. G., Hillmer, S., Stussi-Garaud, C., & Ritzenthaler, C.
- Venue
- The Plant Cell
- Publisher
- Oxford University Press (OUP)
- Published
- 2003-08-08
- Volume
- 15
- Issue
- 9
- Pages
- 2058-2075
- ISSN
- 1040-4651 (ISSN-L)
- 1532-298X
- OpenAlex
- W2104274402
- Cited by
- 202
- References
- 2
- FWCI
- 11.85
- Type
- journal article
- Language
- en
- Open access
- green
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- The paper
- Laporte, C., Vetter, G., Loudes, A.-M., Robinson, D. G., Hillmer, S., Stussi-Garaud, C., & Ritzenthaler, C. (2003). Involvement of the Secretory Pathway and the Cytoskeleton in Intracellular Targeting and Tubule Assembly of Grapevine fanleaf virus Movement Protein in Tobacco BY-2 Cells. The Plant Cell. https://doi.org/10.1105/tpc.013896
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