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Aux/IAA Proteins Contain a Potent Transcriptional Repression Domain

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Tiwari, Shiv B., Hagen, Gretchen, Guilfoyle, Tom J.·2004·The Plant Cell

Abstract

Aux/IAA proteins are short-lived nuclear proteins that repress expression of primary/early auxin response genes in protoplast transfection assays. Repression is thought to result from Aux/IAA proteins dimerizing with auxin response factor (ARF) transcriptional activators that reside on auxin-responsive promoter elements, referred to as AuxREs. Most Aux/IAA proteins contain four conserved domains, designated domains I, II, III, and IV. Domain II and domains III and IV play roles in protein stability and dimerization, respectively. A clear function for domain I had not been established. Results reported here indicate that domain I in Aux/IAA proteins is an active repression domain that is transferable and dominant over activation domains. An LxLxL motif within domain I is important for conferring repression. The dominance of Aux/IAA repression domains over activation domains in ARF transcriptional activators provides a plausible explanation for the repression of auxin response genes via ARF-Aux/IAA dimerization on auxin-responsive promoters.

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Metadata

Authors
Tiwari, S. B., Hagen, G., & Guilfoyle, T. J.
Venue
The Plant Cell
Published
2004-01-23
Volume
16
Issue
2
Pages
533-543
ISSN
  • 1040-4651 (ISSN-L)
  • 1532-298X
OpenAlex
W2144249817
Cited by
584
References
33
FWCI
36.36
Type
journal article
Language
en
Open access
green

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CoChat. (2026). Citation check: Aux/IAA Proteins Contain a Potent Transcriptional Repression Domain. https://app.cochat.ai/papers/10.1105/tpc.017384
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Tiwari, S. B., Hagen, G., & Guilfoyle, T. J. (2004). Aux/IAA Proteins Contain a Potent Transcriptional Repression Domain. The Plant Cell. https://doi.org/10.1105/tpc.017384

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