The DOI matches its Crossref record; 9 of the 9 references it cites were found in a registry or an index.
Highly Specific Gene Silencing by Artificial MicroRNAs in Arabidopsis
Open accessAbstract
Plant microRNAs (miRNAs) affect only a small number of targets with high sequence complementarity, while animal miRNAs usually have hundreds of targets with limited complementarity. We used artificial miRNAs (amiRNAs) to determine whether the narrow action spectrum of natural plant miRNAs reflects only intrinsic properties of the plant miRNA machinery or whether it is also due to past selection against natural miRNAs with broader specificity. amiRNAs were designed to target individual genes or groups of endogenous genes. Like natural miRNAs, they had varying numbers of target mismatches. Previously determined parameters of target selection for natural miRNAs could accurately predict direct targets of amiRNAs. The specificity of amiRNAs, as deduced from genome-wide expression profiling, was as high as that of natural plant miRNAs, supporting the notion that extensive base pairing with targets is required for plant miRNA function. amiRNAs make an effective tool for specific gene silencing in plants, especially when several related, but not identical, target genes need to be downregulated. We demonstrate that amiRNAs are also active when expressed under tissue-specific or inducible promoters, with limited nonautonomous effects. The design principles for amiRNAs have been generalized and integrated into a Web-based tool (http://wmd.weigelworld.org).
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Antagonistic Regulation of PIN Phosphorylation by PP2A and PINOID Directs Auxin Flux
Michniewicz, M., Zago, M. K., Abas, L., Weijers, D., Schweighofer, A., Meskiene, I., Heisler, M. G., Ohno, C., Zhang, J., Huang, F., et al. (2007) · Cell · doi:10.1016/j.cell.2007.07.033 · Cited by 678
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Metadata
- Authors
- Schwab, R., Ossowski, S., Riester, M., Warthmann, N., & Weigel, D.
- Venue
- The Plant Cell
- Publisher
- Oxford University Press (OUP)
- Published
- 2006-03-10
- Volume
- 18
- Issue
- 5
- Pages
- 1121-1133
- ISSN
- 1040-4651 (ISSN-L)
- 1532-298X
- OpenAlex
- W2096183596
- Cited by
- 1,346
- References
- 9
- FWCI
- 61.96
- Type
- journal article
- Language
- en
- Open access
- hybrid
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