Widespread translational inhibition by plant miRNAs and siRNAs
Abstract
High complementarity between plant microRNAs (miRNAs) and their messenger RNA targets is thought to cause silencing, prevalently by endonucleolytic cleavage. We have isolated Arabidopsis mutants defective in miRNA action. Their analysis provides evidence that plant miRNA-guided silencing has a widespread translational inhibitory component that is genetically separable from endonucleolytic cleavage. We further show that the same is true of silencing mediated by small interfering RNA (siRNA) populations. Translational repression is effected in part by the ARGONAUTE proteins AGO1 and AGO10. It also requires the activity of the microtubule-severing enzyme katanin, implicating cytoskeleton dynamics in miRNA action, as recently suggested from animal studies. Also as in animals, the decapping component VARICOSE (VCS)/Ge-1 is required for translational repression by miRNAs, which suggests that the underlying mechanisms in the two kingdoms are related.
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In Arabidopsis thaliana, microRNAs and small interfering RNAs are essential regulators of plant development, guide DNA methylation, and act as transcriptional repressors.
“As proven in the model species Arabidopsis thaliana, microRNAs (miRNA) and small interfering RNAs (siRNA) are essential regulators of plant development, guide DNA methylation in plants (especially de novo methylation), and act as transcriptional repressors [127,128].”
From Somaclonal Variation—Advantage or Disadvantage in Micropropagation of the Medicinal Plants (2023) · cites this paper as [127]
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Somaclonal Variation—Advantage or Disadvantage in Micropropagation of the Medicinal Plants
Duta-Cornescu, G., Constantin, N., Pojoga, D.-M., Nicuta, D., & Simon-Gruita, A. (2023) · International Journal of Molecular Sciences · doi:10.3390/ijms24010838 · Cited by 108 · see citing works
“As proven in the model species Arabidopsis thaliana, microRNAs (miRNA) and small interfering RNAs (siRNA) are essential regulators of plant development, guide DNA methylation in plants (especially de novo methylation), and act as transcriptional repressors [127,128].”
BackgroundBraun, J. E., Huntzinger, E., & Izaurralde, E. (2012) · Cold Spring Harbor Perspectives in Biology · doi:10.1101/cshperspect.a012328 · Cited by 61 · see citing works
- Authors
- Brodersen, P., Sakvarelidze-Achard, L., Bruun-Rasmussen, M., Dunoyer, P., Yamamoto, Y. Y., Sieburth, L., & Voinnet, O.
- Affiliations
- Centre National de la Recherche Scientifique (FR)
- Corresponding author
- Voinnet, Olivier
- Venue
- Science
- Published
- 2008-05-30
- Volume
- 320
- Issue
- 5880
- Pages
- 1185-1190
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2011518477
- Cited by
- 1,477
- References
- 0
- Citations by year
- 2026: 24 · 2025: 40 · 2024: 36 · 2023: 56 · 2022: 63 · 2021: 82 · 2020: 66 · 2019: 60 · 2018: 62 · 2017: 87 · 2016: 89 · 2015: 98 · 2014: 112 · 2013: 120 · 2012: 116
- FWCI
- 145.53
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Science (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Molecular Biology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Chromosomal and Genetic Variations
- Keywords
- plant microRNAs, siRNA, protein synthesis inhibition, RNA silencing, endonucleolytic cleavage, AGO1, Argonaute proteins, katanin, microtubule dynamics, cytoskeleton, mRNA decapping, Arabidopsis mutants
- MeSH terms
- Katanin, Adenosine Triphosphatases, Mutation, RNA Caps, Protein Biosynthesis, Arabidopsis, Gene Expression Regulation, Plant, RNA, Plant, Arabidopsis Proteins, RNA Interference, RNA, Small Interfering, MicroRNAs, Green Fluorescent Proteins, Argonaute Proteins
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