The DOI matches its Crossref record; 4 of the 4 references it cites were found in a registry or an index.
Calcium-Dependent Protein Kinases Regulate the Production of Reactive Oxygen Species by Potato NADPH Oxidase
Open accessAbstract
Reactive oxygen species (ROS) are implicated in plant innate immunity. NADPH oxidase (RBOH; for Respiratory Burst Oxidase Homolog) plays a central role in the oxidative burst, and EF-hand motifs in the N terminus of this protein suggest possible regulation by Ca2+. However, regulatory mechanisms are largely unknown. We identified Ser-82 and Ser-97 in the N terminus of potato (Solanum tuberosum) St RBOHB as potential phosphorylation sites. An anti-phosphopeptide antibody (pSer82) indicated that Ser-82 was phosphorylated by pathogen signals in planta. We cloned two potato calcium-dependent protein kinases, St CDPK4 and St CDPK5, and mass spectrometry analyses showed that these CDPKs phosphorylated only Ser-82 and Ser-97 in the N terminus of St RBOHB in a calcium-dependent manner. Ectopic expression of the constitutively active mutant of St CDPK5, St CDPK5VK, provoked ROS production in Nicotiana benthamiana leaves. The CDPK-mediated ROS production was disrupted by knockdown of Nb RBOHB in N. benthamiana. The loss of function was complemented by heterologous expression of wild-type potato St RBOHB but not by a mutant (S82A/S97A). Furthermore, the heterologous expression of St CDPK5VK phosphorylated Ser-82 of St RBOHB in N. benthamiana. These results suggest that St CDPK5 induces the phosphorylation of St RBOHB and regulates the oxidative burst.
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Cites
NOX enzymes and the biology of reactive oxygen
Lambeth, J. D. (2004) · Nature Reviews Immunology · doi:10.1038/nri1312 · Cited by 3,125
A Ca2+-activated NADPH Oxidase in Testis, Spleen, and Lymph Nodes
Bánfi, B., Molnár, G., Maturana, A., Steger, K., Hegedûs, B., Demaurex, N., & Krause, K.-H. (2001) · Journal of Biological Chemistry · doi:10.1074/jbc.m103034200 · Cited by 622
Mechanism of Ca2+ Activation of the NADPH Oxidase 5 (NOX5)
Bánfi, B., Tirone, F., Durussel, I., Knisz, J., Moskwa, P., Molnár, G. Z., Krause, K.-H., & Cox, J. A. (2004) · Journal of Biological Chemistry · doi:10.1074/jbc.m310268200 · Cited by 389
Takeya, R., Ueno, N., Kami, K., Taura, M., Kohjima, M., Izaki, T., Nunoi, H., & Sumimoto, H. (2003) · Journal of Biological Chemistry · doi:10.1074/jbc.m212856200 · Cited by 380
Cited by
The Language of Calcium Signaling
Dodd, A. N., Kudla, J., & Sanders, D. (2010) · Annual Review of Plant Biology · doi:10.1146/annurev-arplant-070109-104628 · Cited by 1,317
Sumimoto, H. (2008) · The FEBS Journal · doi:10.1111/j.1742-4658.2008.06488.x · Cited by 721
Regulation of the NADPH Oxidase RBOHD During Plant Immunity
Kadota, Y., Shirasu, K., & Zipfel, C. (2015) · Plant and Cell Physiology · doi:10.1093/pcp/pcv063 · Cited by 641
Zhang, Y., Zhu, H., Zhang, Q., Li, M., Yan, M., Wang, R., Wang, L., Welti, R., Zhang, W., & Wang, X. (2009) · The Plant Cell · doi:10.1105/tpc.108.062992 · Cited by 580
The role of the cell wall in plant immunity
Malinovsky, F. G., Fangel, J. U., & Willats, W. G. T. (2014) · Frontiers in Plant Science · doi:10.3389/fpls.2014.00178 · Cited by 555
Metadata
- Authors
- Kobayashi, M., Ohura, I., Kawakita, K., Yokota, N., Fujiwara, M., Shimamoto, K., Doke, N., & Yoshioka, H.
- Venue
- The Plant Cell
- Publisher
- Oxford University Press (OUP)
- Published
- 2007-03-01
- Volume
- 19
- Issue
- 3
- Pages
- 1065-1080
- ISSN
- 1040-4651 (ISSN-L)
- 1532-298X
- OpenAlex
- W2117880739
- Cited by
- 670
- References
- 4
- FWCI
- 33.88
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Kobayashi, M., Ohura, I., Kawakita, K., Yokota, N., Fujiwara, M., Shimamoto, K., Doke, N., & Yoshioka, H. (2007). Calcium-Dependent Protein Kinases Regulate the Production of Reactive Oxygen Species by Potato NADPH Oxidase. The Plant Cell. https://doi.org/10.1105/tpc.106.048884
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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