The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Arabidopsis PHYTOCHROME INTERACTING FACTOR Proteins Promote Phytochrome B Polyubiquitination by COP1 E3 Ligase in the Nucleus
Open accessAbstract
Many plant photoresponses from germination to shade avoidance are mediated by phytochrome B (phyB). In darkness, phyB exists as the inactive Pr in the cytosol but upon red (R) light treatment, the active Pfr translocates into nuclei to initiate signaling. Degradation of phyB Pfr likely regulates signal termination, but the mechanism is not understood. Here, we show that phyB is stable in darkness, but in R, a fraction of phyB translocates into nuclei and becomes degraded by 26S proteasomes. Nuclear phyB degradation is mediated by COP1 E3 ligase, which preferentially interacts with the PhyB N-terminal region (PhyB-N). PhyB-N polyubiquitination by CONSTITUTIVE PHOTOMORPHOGENIC1 (COP1) in vitro can be enhanced by different PHYTOCHROME INTERACTING FACTOR (PIF) proteins that promote COP1/PhyB interaction. Consistent with these results, nuclear phyB accumulates to higher levels in pif single and double mutants and in cop1-4. Our results identify COP1 as an E3 ligase for phyB and other stable phytochromes and uncover the mechanism by which PIFs negatively regulate phyB levels.
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Citations
Cites
Kruse, J.-P., & Gu, W. (2009) · Cell · doi:10.1016/j.cell.2009.04.050 · Cited by 1,610 · cited 1×
“The stimulatory effect of PIFs on phyB ubiquitination is reminiscent of the modulation of p53 ubiquitination by regulatory factors in animal cells (Sui et al., 2004; Allende-Vega et al., 2007; Kruse and Gu, 2009).”
Background
Cited by
Photoreceptor Signaling Networks in Plant Responses to Shade
Casal, J. J. (2013) · Annual Review of Plant Biology · doi:10.1146/annurev-arplant-050312-120221 · Cited by 865
Phytochrome Signaling Mechanisms
Li, J., Li, G., Wang, H., & Wang Deng, X. (2011) · The Arabidopsis Book · doi:10.1199/tab.0148 · Cited by 561
Li, Y.-Y., Mao, K., Zhao, C., Zhao, X.-Y., Zhang, H.-L., Shu, H.-R., & Hao, Y.-J. (2012) · Plant Physiology · doi:10.1104/pp.112.199703 · Cited by 489
Phytochrome signaling mechanisms and the control of plant development
Chen, M., & Chory, J. (2011) · Trends in Cell Biology · doi:10.1016/j.tcb.2011.07.002 · Cited by 448
E3 Ubiquitin Ligases: Ubiquitous Actors in Plant Development and Abiotic Stress Responses
Shu, K., & Yang, W. (2017) · Plant and Cell Physiology · doi:10.1093/pcp/pcx071 · Cited by 322
Metadata
- Authors
- Jang, I.-C., Henriques, R., Seo, H. S., Nagatani, A., & Chua, N.-H.
- Venue
- The Plant Cell
- Publisher
- Oxford University Press (OUP)
- Published
- 2010-07-01
- Volume
- 22
- Issue
- 7
- Pages
- 2370-2383
- ISSN
- 1040-4651 (ISSN-L)
- 1532-298X
- OpenAlex
- W2137501007
- Cited by
- 227
- References
- 1
- FWCI
- 20.01
- Type
- journal article
- Language
- en
- Open access
- green
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- Jang, I.-C., Henriques, R., Seo, H. S., Nagatani, A., & Chua, N.-H. (2010). Arabidopsis PHYTOCHROME INTERACTING FACTOR Proteins Promote Phytochrome B Polyubiquitination by COP1 E3 Ligase in the Nucleus. The Plant Cell. https://doi.org/10.1105/tpc.109.072520
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