Cations Inhibit Specifically Type I 5α-Reductase Found in Human Skin
Open accessAbstract
Steroid 5 alpha-reductase catalyzes the reduction of testosterone into the very potent androgen dihydrotestosterone. Previously, we showed that human type I 5 alpha-reductase is expressed mainly in the skin, whereas a type II 5 alpha-reductase is more specifically expressed in the prostate. To assess the possible differential effects of various cations on the two types of 5 alpha-reductase, we constructed expression vectors and transfected them into SW-13 cells, a human adrenal carcinoma cell line containing negligible endogenous 5 alpha-reductase activity. The expressed 5 alpha-reductases were analyzed for their sensitivity to Li, Ca, Cd, Cu, Mg, Mn, Ni, Zn, and Fe. The results showed that type I 5 alpha-reductase was strongly inhibited by Cd, Cu, and Zn and moderately inhibited by Ni and Fe, with 50% inhibitory concentration values of 0.9, 1.9, 2.0, 169.2, and 174.3 microM, respectively. In contrast, type II 5 alpha-reductase activity was inhibited only by Cu, with a 50% inhibitory concentration value of 19.2 microM. The data showed that cations could specifically control 5 alpha-reductase activity expression, which is more strongly inhibited in a target tissue, especially the skin.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
The presence of SRD5A1 allows for local production of DHT, with the 5α-dione pathway bypassing T being the preferred route.
“The presence of SRD5A1 allows for the local production of DHT (Luu-The et al., 1994), with the 5α-dione pathway bypassing T being the preferred route (Samson et al., 2010, Sugimoto et al., 1995) (Fig. 6).”
From Intracrine androgen biosynthesis, metabolism and action revisited (2018) · cites this paper as Sugimoto et al., 1995
Cites
DNA sequencing with chain-terminating inhibitors
Sanger, F., Nicklen, S., & Coulson, A. R. (1977) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.74.12.5463 · Cited by 68,572 · see citing works
Current protocols in molecular biology
Sargent, T. (1988) · Analytical Biochemistry · doi:10.1016/0003-2697(88)90147-9 · Cited by 7,298 · see citing works
Steroid 5α-Reductase Deficiency in Man: An Inherited Form of Male Pseudohermaphroditism
Imperato-McGinley, J., Guerrero, L., Gautier, T., & Peterson, R. E. (1974) · Science · doi:10.1126/science.186.4170.1213 · Cited by 1,258 · see citing works
Tissue distribution and ontogeny of steroid 5 alpha-reductase isozyme expression.
Thigpen, A. E., Silver, R. I., Guileyardo, J. M., Casey, M. L., McConnell, J. D., & Russell, D. W. (1993) · Journal of Clinical Investigation · doi:10.1172/jci116665 · Cited by 727 · see citing works
Labrie, F. (1991) · Molecular and Cellular Endocrinology · doi:10.1016/0303-7207(91)90116-a · Cited by 685 · see citing works
Self-citationHuman Growth Hormone as a Reporter Gene in Regulation Studies Employing Transient Gene Expression
Selden, R. F., Howie, K. B., Rowe, M. E., Goodman, H. M., & Moore, D. D. (1986) · Molecular and Cellular Biology · doi:10.1128/mcb.6.9.3173 · Cited by 663 · see citing works
Deletion of steroid 5α-reductase 2 gene in male pseudohermaphroditism
Andersson, S., Berman, D. M., Jenkins, E. P., & Russell, D. W. (1991) · Nature · doi:10.1038/354159a0 · Cited by 643 · see citing works
Andersson, S., & Russell, D. W. (1990) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.87.10.3640 · Cited by 501 · see citing works
Molecular genetics of steroid 5 alpha-reductase 2 deficiency
Thigpen, A. E., Davis, D. L., Milatovich, A., Mendonca, B. B., Imperato-McGinley, J., Griffin, J. E., Francke, U., Wilson, J. D., & Russell, D. W. (1992) · Journal of Clinical Investigation · doi:10.1172/jci115954 · Cited by 422 · see citing works
Sansone, G., & Reisner, R. M. (1971) · Journal of Investigative Dermatology · doi:10.1111/1523-1747.ep12261252 · Cited by 300 · see citing works
Structure of human type II 5 alpha-reductase gene
Labrie, F., Sugimoto, Y., Luu-The, V., Simard, J., Lachance, Y., Bachvarov, D. R., Leblanc, G., Durocher, F., & Paquet, N. (1992) · Endocrinology · doi:10.1210/endo.131.3.1505484 · Cited by 203 · see citing works
Self-citationRegulation of Human Hair Growth by Steroid Hormones. I. Testosterone Metabolism in Isolated Hairs
Schweikert, H. U., & Wilson, J. D. (1974) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jcem-38-5-811 · Cited by 202 · see citing works
Lachance, Y., Luu-The, V, Labrie, C., Simard, J., Dumont, M., de Launoit, Y., Guérin, S., Leblanc, G., & Labrie, F. (1990) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)30528-8 · Cited by 201 · see citing works
Self-citationANDROGEN PRODUCTION AND SKIN METABOLISM IN HIRSUTISM
Kuttenn, F., Mowszowicz, I., SCHAISON, G., & MAUVAIS-JARVIS, P. (1977) · Journal of Endocrinology · doi:10.1677/joe.0.0750083 · Cited by 195 · see citing works
Jenkins, E. P., Hsieh, C., Milatovich, A., Normington, K., Berman, D. M., Francke, U., & Russell, D. W. (1991) · Genomics · doi:10.1016/0888-7543(91)90038-g · Cited by 152 · see citing works
Kavanagh, J. P. (1985) · Reproduction · doi:10.1530/jrf.0.0750035 · Cited by 148 · see citing works
Characterization, Expression, and Immunohistochemical Localization of 5α-Reductase in Human Skin
Luu-The, V., Sugimoto, Y., Puy, L., Labrie, Y., Solache, I. L., Singh, M., & Labrie, F. (1994) · Journal of Investigative Dermatology · doi:10.1111/1523-1747.ep12371766 · Cited by 118 · see citing works
Self-citationItami, S., Kurata, S., Sonoda, T., & Takayasu, S. (1991) · Journal of Investigative Dermatology · doi:10.1111/1523-1747.ep12514729 · Cited by 114 · see citing works
Inhibition of 5α-reductase activity in human skin by zinc and azelaic acid
Stamatiadis, D., BULTEAU-PORTOIS, M.-C., & Mowszowicz, I. (1988) · British Journal of Dermatology · doi:10.1111/j.1365-2133.1988.tb03474.x · Cited by 97 · see citing works
Labrie, F., Bélanger, A., Simard, J., Labrie, C., & Dupont, A. (1993) · Cancer · doi:10.1002/1097-0142(19930201)71:3+ · Cited by 93 · see citing works
Self-citationZinc and the steroid endocrinology of the human prostate
Habib, F. K. (1978) · Journal of Steroid Biochemistry · doi:10.1016/0022-4731(78)90608-8 · Cited by 56 · see citing works
The effect of zinc on the 5α-reduction of testosterone by the hyperplastic human prostate gland
Leake, A., CHISHOLM, G. D., & Habib, F. K. (1984) · Journal of Steroid Biochemistry · doi:10.1016/0022-4731(84)90138-9 · Cited by 46 · see citing works
The effect of zinc on the sebum secretion rate
Demetree, J., Safer, L. F., & Artis, W. M. (1980) · Acta Dermato Venereologica · doi:10.2340/0001555560166169 · Cited by 24 · see citing works
Hirsutism: pilosebaceous unit dysregulation. Role of peripheral and glandular factors
Toscano, V. (1991) · Journal of Endocrinological Investigation · doi:10.1007/bf03350293 · Cited by 16 · see citing works
Studies of zinc in normal and neoplastic prostatic tissues.
Rosoff, B. (1981) · PubMed · Cited by 15 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Intracrine androgen biosynthesis, metabolism and action revisited
Schiffer, L., Arlt, W., & Storbeck, K.-H. (2018) · Molecular and Cellular Endocrinology · doi:10.1016/j.mce.2017.08.016 · Cited by 217 · see citing works
“…5a-dione due to the higher affinity and catalytic efficiency of SRD5A1 for A4 than for T (Andersson and Russell, 1990; Russell and Wilson, 1994; Sugimoto et al., 1995), which is followed by the conversion of 5a-dione to DHT by reduction of the 17-ketone (Chang et al., 2011; Luu-The and Labrie,…”
Androgen biosynthetic pathways in the human prostate
Luu-The, V., Bélanger, A., & Labrie, F. (2008) · Best Practice & Research Clinical Endocrinology & Metabolism · doi:10.1016/j.beem.2008.01.008 · Cited by 169 · see citing works
Samson, M., Labrie, F., Zouboulis, C. C., & Luu-The, V. (2010) · Journal of Investigative Dermatology · doi:10.1038/jid.2009.225 · Cited by 42 · see citing works
Self-citation
- Authors
- Sugimoto, Y., López-Solache, I., Labrie, F., & Luu-The, V. A. N.
- Affiliations
- Centre de recherche du CHU de Québec-Université Laval (CA)
- Université Laval (CA)
- ORCID
- Sugimoto, Yasuro
- Venue
- Journal of Investigative Dermatology
- Publisher
- Elsevier BV
- Published
- 1995-05
- Volume
- 104
- Issue
- 5
- Pages
- 775-778
- ISSN
- 0022-202X (ISSN-L)
- OpenAlex
- W1971835761
- Cited by
- 53
- References
- 25
- Citations by year
- 2026: 2 · 2025: 2 · 2022: 3 · 2021: 2 · 2020: 1 · 2019: 2 · 2018: 4 · 2017: 2 · 2016: 2 · 2015: 1 · 2014: 1 · 2013: 2
- FWCI
- 0.96
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Journal of Investigative Dermatology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Urology › Hair Growth and Disorders
- Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Prostate Cancer Treatment and Research
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Hormonal and reproductive studies
- Keywords
- cation inhibition, dihydrotestosterone, testosterone suppression, human skin, metal ions, cadmium, copper, zinc, enzyme inhibition, SW13 cells, enzyme expression
- MeSH terms
- Anions, Base Sequence, Cadmium, Cations, Copper, Humans, Hydrogen-Ion Concentration, Iron, Molecular Sequence Data, Nickel, Skin, 3-Oxo-5-alpha-Steroid 4-Dehydrogenase, Transfection, Tumor Cells, Cultured, Zinc, 5-alpha Reductase Inhibitors
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
