Characterization, Expression, and Immunohistochemical Localization of 5α-Reductase in Human Skin
Open accessAbstract
Human skin has been shown to contain a high level of 5 alpha-reductase activity, the enzyme that catalyses the conversion of the weak androgen testosterone into dihydrotestosterone, the most potent androgen. Because two types of 5 alpha-reductase genes have been characterized in humans, we have cloned 5 alpha-reductase cDNAs from adult human keratinocyte and skin fibroblast cDNA libraries to identify and gain better knowledge of the 5 alpha-reductase expressed in normal human skin. Nucleotide sequence analysis shows that the clones obtained correspond to the type I 5 alpha-reductase. RNase protection analysis using (poly A)+ RNA obtained from human skin and prostate also confirms that type I 5 alpha-reductase is the predominant type expressed in normal skin, whereas type II 5 alpha-reductase is the major form found in the prostate. Following polymerase chain reaction amplification of human keratinocyte and skin fibroblast cDNA, a low level of type II 5 alpha-reductase cDNA has been detected. Using antipeptide antibodies raised in rabbits against the peptide sequence covering amino acids 227 -240 to perform immunohistochemical localization of 5 alpha-reductase, we have found that 5 alpha-reductase is distributed in sweat and sebaceous glands, as well as in the epidermal cell layers, thus providing the basis for the important role of androgens in human skin and its appendages.
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The presence of SRD5A1 allows for local production of DHT, with the 5α-dione pathway bypassing T being the preferred route.
“The presence of SRD5A1 allows for the local production of DHT (Luu-The et al., 1994), with the 5α-dione pathway bypassing T being the preferred route (Samson et al., 2010, Sugimoto et al., 1995) (Fig. 6).”
From Intracrine androgen biosynthesis, metabolism and action revisited (2018) · cites this paper as Luu-The et al., 1994
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- Authors
- Luu-The, V., Sugimoto, Y., Puy, L., Labrie, Y., Solache, I. L., Singh, M., & Labrie, F.
- Affiliations
- Université Laval (CA)
- Venue
- Journal of Investigative Dermatology
- Publisher
- Elsevier BV
- Published
- 1994-02
- Volume
- 102
- Issue
- 2
- Pages
- 221-226
- ISSN
- 0022-202X (ISSN-L)
- OpenAlex
- W2022602285
- Cited by
- 118
- References
- 54
- Citations by year
- 2026: 1 · 2023: 2 · 2018: 1 · 2017: 2 · 2016: 2 · 2015: 3 · 2014: 3 · 2013: 1
- FWCI
- 12.49
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Journal of Investigative Dermatology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Urology › Hair Growth and Disorders
- Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Prostate Cancer Treatment and Research
- Health Sciences › Medicine › Reproductive Medicine › Sperm and Testicular Function
- Keywords
- 5α-reductase, human skin, keratinocytes, dermal fibroblasts, dihydrotestosterone, androgen metabolism, sweat glands, sebaceous glands, epidermis, immunolocalization, cDNA cloning, prostate
- MeSH terms
- Base Sequence, Cells, Cultured, DNA, Fibroblasts, Humans, Immunohistochemistry, Male, Molecular Sequence Data, Oxidoreductases, Prostate, Sebaceous Glands, Skin, Sweat Glands, Immunoblotting, Keratinocytes, Polymerase Chain Reaction, Cholestenone 5 alpha-Reductase
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