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Cited by 300

The DOI matches its Crossref record; 34 of the 34 references it cites were found in a registry or an index.

Comparison of methods used for assessing the viability and vitality of yeast cells

Kwolek-Mirek, Magdalena, Zadrag-Tecza, Renata·2014·FEMS Yeast Research

Abstract

Determination of cell viability is the most commonly used method for assessing the impact of various types of stressors in toxicity research and in industrial microbiology studies. Viability is defined as a percentage of live cells in a whole population. Although cell death is one of the consequences of toxicity, chemical or physical factors may exert their toxic effects through a number of cellular alterations that may compromise cell ability to divide without necessarily leading to cell death. This aspect represents the term 'cell vitality' defined as physiological capabilities of cells. It is important to note that cell viability and cell vitality represent two different aspects of cell functions, and both are required for the estimation of the physiological state of a cell after exposure to various types of stressors and chemical or physical factors. In this paper, we introduced a classification of available methods for estimating both viability and vitality in Saccharomyces cerevisiae yeast cells (wild-type and Δsod1 mutant) in which the effects of selected oxidants causing oxidative stress is evaluated. We present the advantages as well as disadvantages of the selected methods and assess their usefulness in different types of research.

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Metadata

Authors
Kwolek-Mirek, M., & Zadrag-Tecza, R.
Venue
FEMS Yeast Research
Published
2014-09
Volume
14
Issue
7
Pages
n/a-n/a
ISSN
1567-1356 (ISSN-L)
OpenAlex
W2102202691
Cited by
300
References
34
FWCI
2.31
Type
journal article
Language
en
Open access
closed

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CoChat. (2026). Citation check: Comparison of methods used for assessing the viability and vitality of yeast cells. https://app.cochat.ai/papers/10.1111/1567-1364.12202
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Kwolek-Mirek, M., & Zadrag-Tecza, R. (2014). Comparison of methods used for assessing the viability and vitality of yeast cells. FEMS Yeast Research. https://doi.org/10.1111/1567-1364.12202

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