Genoprotective effects of essential oil compounds against oxidative and methylated DNA damage in human colon cancer cells
Abstract
Essential oils (EO) are widely used in foods as flavoring and preservative agents. Many of the biological activities of EO have been attributed to major essential oil compounds (EOC) but their direct interaction with colonic epithelial cells and their genotoxic and genoprotective effects are not well established. In this study, the cytotoxicity and genotoxicity of EOC including nerolidol, thymol, geraniol, methylisoeugenol, eugenol, linalool, and a commercial blend (Agolin) were determined. Furthermore, the genoprotective effects of EOC against oxidative and methylating damage were assessed using the comet assay in HT-29 colorectal adenocarcinoma cells. The majority of EOC were cytotoxic to HT-29 cells at or above 250 ppm after 24 hr exposure. At noncytotoxic doses, none of the EOC was genotoxic in the comet assay. Genoprotection against oxidative DNA damage was observed for nerolidol (at 62.5 ppm), thymol (at 12.5 ppm), geraniol, and methylisoeugenol (both at 125 ppm), as well as linalool and Agolin (both at 250 ppm). Thymol was the most protective compound against oxidative DNA damage and geraniol (at 125 ppm) also protected cells against methylating DNA damage. This study highlights the potential of EOC such as thymol to protect the colonic epithelium against oxidative DNA damage and geraniol against methylating DNA damage. Further in vivo studies are needed to confirm these findings for safety and efficacy to exploit their potential pharmaceutical or nutraceutical uses for colonic health.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Eugenol inhibits tumor proliferation and formation, increases reactive oxygen species (ROS), generates apoptosis, and has a genotoxic effect in different cancer cells.
“Eugenol inhibits tumor proliferation and formation, increases reactive oxygen species (ROS), generates apoptosis, and has a genotoxic effect in different cancer cells [23,29,30,31].”
From Clove essential oil (Syzygium aromaticum L. Myrtaceae): Extraction, chemical composition, food applications, and essential bioactivity for human health (2021) · cites this paper as [31]
Cites
Essential oils: Their antibacterial properties and potential applications in foods—A review
Burt, S. (2004) · International Journal of Food Microbiology · doi:10.1016/j.ijfoodmicro.2004.03.022 · Cited by 10,488 · see citing works
Reactive oxygen species in cancer
Liou, G., & Störz, P. (2010) · Free Radical Research · doi:10.3109/10715761003667554 · Cited by 3,394 · see citing works
The Comet Assay for DNA Damage and Repair: Principles, Applications, and Limitations
Collins, A. R. (2004) · Molecular Biotechnology · doi:10.1385/mb:26:3:249 · Cited by 2,892 · see citing works
Essential oils used in aromatherapy: A systemic review
Ali, B., Al‐Wabel, N. A., Shams, S., Ahamad, A., Khan, S. A., & Anwar, F. (2015) · Asian Pacific Journal of Tropical Biomedicine · doi:10.1016/j.apjtb.2015.05.007 · Cited by 1,184 · see citing works
Endogenous DNA damage in humans: a review of quantitative data
Bont, R. D. (2004) · Mutagenesis · doi:10.1093/mutage/geh025 · Cited by 1,065 · see citing works
Guidelines for accurate EC50/IC50 estimation
Sebaugh, J. L. (2010) · Pharmaceutical Statistics · doi:10.1002/pst.426 · Cited by 949 · see citing works
Essential Oils: Extraction, Bioactivities, and Their Uses for Food Preservation
Tongnuanchan, P., & Benjakul, S. (2014) · Journal of Food Science · doi:10.1111/1750-3841.12492 · Cited by 884 · see citing works
Measuring oxidative damage to DNA and its repair with the comet assay
Collins, A. R. (2013) · Biochimica et Biophysica Acta (BBA) - General Subjects · doi:10.1016/j.bbagen.2013.04.022 · Cited by 337 · see citing works
Teixeira, B., Marques, A., Ramos, C., Batista, I., Serrano, C., Matos, O., Neng, N. R., Nogueira, J. M. F., Saraiva, J. A., & Nunes, M. L. (2011) · Industrial Crops and Products · doi:10.1016/j.indcrop.2011.08.011 · Cited by 225 · see citing works
Oxidative Stress in the Pathogenesis of Colorectal Cancer: Cause or Consequence?
Perše, M. (2013) · BioMed Research International · doi:10.1155/2013/725710 · Cited by 221 · see citing works
Deb, D. D., Parimala, G., Devi, S. S., & Chakraborty, T. (2011) · Chemico-Biological Interactions · doi:10.1016/j.cbi.2011.05.009 · Cited by 198 · see citing works
Essential oil of Myrtus communis L. as a potential antioxidant and antimutagenic agents
Mimica-Dukić, N., Bugarin, D., Grbović, S., Mitić-Ćulafić, D., Vuković-Gačić, B., Orčić, D., Jovin, E., & Couladis, M. (2010) · Molecules · doi:10.3390/molecules15042759 · Cited by 195 · see citing works
Dietary plant bioactives for poultry health and productivity
Wallace, R. J., Oleszek, W. A., Franz, C., Hahn, I., Başer, K. H. C., Máthé, Á., & Teichmann, K. (2010) · British Poultry Science · doi:10.1080/00071668.2010.506908 · Cited by 179 · see citing works
Self-citationÜndeğer, Ü., Başaran, A. A., Degen, G. H., & Başaran, N. (2009) · Food and Chemical Toxicology · doi:10.1016/j.fct.2009.05.020 · Cited by 172 · see citing works
Mitić-Ćulafić, D., Žegura, B., Nikolić, B., Vuković‐Gačić, B., Knežević‐Vukčević, J., & Filipič, M. (2008) · Food and Chemical Toxicology · doi:10.1016/j.fct.2008.11.015 · Cited by 168 · see citing works
Anti-cancer properties of phenolics from apple waste on colon carcinogenesis in vitro
McCann, M. J., Gill, C. I. R., Brien, G. O., Rao, J. R., McRoberts, W. C., Hughes, P., McEntee, R., & Rowland, I. R. (2007) · Food and Chemical Toxicology · doi:10.1016/j.fct.2007.01.003 · Cited by 151 · see citing works
Potential anti-cancer effects of virgin olive oil phenolson colorectal carcinogenesis modelsin vitro
Gill, C. I. R., Boyd, A., McDermott, E., et al. (2005) · International Journal of Cancer · doi:10.1002/ijc.21083 · Cited by 151 · see citing works
Ouazzou, A. A., Lorán, S., Bakkali, M., Laglaoui, A., Rota, C., Herrera, A., Pagán, R., & Conchello, P. (2011) · Journal of the Science of Food and Agriculture · doi:10.1002/jsfa.4505 · Cited by 143 · see citing works
Slameňová, D., Horváthová, E., Wsólová, L., Šrámková, M., & Navarová, J. (2009) · Mutation Research/Genetic Toxicology and Environmental Mutagenesis · doi:10.1016/j.mrgentox.2009.05.016 · Cited by 141 · see citing works
Costabile, A., Fava, F., Röytiö, H., Forssten, S. D., Olli, K., Klievink, J., Rowland, I. R., Ouwehand, A. C., Rastall, R. A., Gibson, G. R., et al. (2012) · British Journal of Nutrition · doi:10.1017/s0007114511005782 · Cited by 135 · see citing works
Rahimi, R., Mozaffari, S., & Abdollahi, M. R. (2008) · Digestive Diseases and Sciences · doi:10.1007/s10620-008-0368-x · Cited by 135 · see citing works
Uliasz, T. F., & Hewett, S. J. (2000) · Journal of Neuroscience Methods · doi:10.1016/s0165-0270(00)00248-x · Cited by 118 · see citing works
Effects of plant extracts on antioxidant status and oxidant-induced stress in Caco-2 cells
Aherne, S. A., Kerry, J. P., & O’Brien, N. M. (2007) · British Journal Of Nutrition · doi:10.1017/s0007114507250469 · Cited by 118 · see citing works
Horváthová, E., Navarová, J., Gálová, E., et al. (2014) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf501006y · Cited by 114 · see citing works
Muyima, N. Y. O., Zulu, G., Bhengu, T. T., & Popplewell, D. (2002) · Flavour and Fragrance Journal · doi:10.1002/ffj.1093 · Cited by 113 · see citing works
Kozics, K., Klusová, V., Srančíková, A., Mučaji, P., Slameňová, D., Hunáková, Ľ., Kusznierewicz, B., & Horváthová, E. (2013) · Food Chemistry · doi:10.1016/j.foodchem.2013.04.089 · Cited by 112 · see citing works
The nature and origin of DNA single-strand breaks determined with the comet assay
Horváthová, E., Slameňová, D., Hlincikova, L., et al. (1998) · Mutation Research/DNA Repair · doi:10.1016/s0921-8777(98)00053-6 · Cited by 99 · see citing works
Herbal Medicine for Cough: a Systematic Review and Meta-Analysis
Wagner, L., Cramer, H., Klose, P., et al. (2015) · Complementary Medicine Research · doi:10.1159/000442111 · Cited by 97 · see citing works
Sensitivity of pathogenic and commensal bacteria from the human colon to essential oils
Thapa, D., Losa, R., Zweifel, B., & Wallace, R. J. (2012) · Microbiology · doi:10.1099/mic.0.061127-0 · Cited by 92 · see citing works
Self-citationThe use of the comet assay in the study of human nutrition and cancer
Wasson, G. R., McKelvey‐Martin, V. J., & Downes, C. S. (2008) · Mutagenesis · doi:10.1093/mutage/gen003 · Cited by 80 · see citing works
Llana‐Ruíz‐Cabello, M., Maisanaba, S., Puerto, M., Prieto, A. I., Pichardo, S., Jos, A. M., & Cameán, A. M. (2014) · Food and Chemical Toxicology · doi:10.1016/j.fct.2014.07.013 · Cited by 64 · see citing works
Origanum VulgareInduces Apoptosis in Human Colon Cancer Caco2Cells
Savini, I., Arnone, R., Catani, M. V., et al. (2009) · Nutrition and Cancer · doi:10.1080/01635580802582769 · Cited by 62 · see citing works
Bouassida, K. Z., Trigui, M., Makni, S., Jlaiel, L., & Tounsi, S. (2018) · BioMed Research International · doi:10.1155/2018/7856517 · Cited by 61 · see citing works
DNA Alkylation and Repair in the Large Bowel: Animal and Human Studies
Povey, A. C., Harrison, K., Badawi, A. F., Cooper, D. P., Hall, C. N., Jackson, P. E., Lees, N. P., O'Connor, P. J., & Margison, G. P. (2002) · Journal of Nutrition · doi:10.1093/jn/132.11.3518s · Cited by 60 · see citing works
Effects of extract and essential oil of Rosmarinus officinalis L. on TNBS-induced colitis in rats.
Minaiyan, M., Ghannadi, A., Afsharipour, M., et al. (2011) · PubMed · Cited by 59 · see citing works
Antioxidant, antimicrobial and antiproliferative activities of Anthemis palestina essential oil
Bardaweel, S. K., Tawaha, K., & Hudaib, M. (2014) · BMC Complementary and Alternative Medicine · doi:10.1186/1472-6882-14-297 · Cited by 59 · see citing works
Antigenotoxic and antioxidant activities of palmarosa and citronella essential oils
Sinha, S. K., Biswas, D., & Mukherjee, A. (2011) · Journal of Ethnopharmacology · doi:10.1016/j.jep.2011.08.046 · Cited by 57 · see citing works
The eucalyptus oil ingredient 1,8-cineol induces oxidative DNA damage
Dörsam, B., Wu, C., Efferth, T., Kaina, B., & Fahrer, J. (2014) · Archives of Toxicology · doi:10.1007/s00204-014-1281-z · Cited by 53 · see citing works
Bidinotto, L. T., Costa, C. A. R. A., Salvadori, D. M. F., et al. (2010) · Journal of Applied Toxicology · doi:10.1002/jat.1593 · Cited by 51 · see citing works
Baker, J., Brown, K., Rajendiran, E., Yip, A., DeCoffe, D., Dai, C., Molcan, E., Chittick, S. A., Ghosh, S., Mahmoud, S. S., et al. (2012) · American Journal of Physiology-Gastrointestinal and Liver Physiology · doi:10.1152/ajpgi.00327.2011 · Cited by 44 · see citing works
Maisanaba, S., Prieto, A. I., Puerto, M., et al. (2015) · Mutation Research/Genetic Toxicology and Environmental Mutagenesis · doi:10.1016/j.mrgentox.2015.05.005 · Cited by 41 · see citing works
Mellado-García, P., Maisanaba, S., Puerto, M., Prieto, A. I., Marcos, R., Pichardo, S., & Cameán, A. M. (2016) · Food and Chemical Toxicology · doi:10.1016/j.fct.2016.12.007 · Cited by 38 · see citing works
Thapa, D., Louis, P., Losa, R., Zweifel, B., & Wallace, R. J. (2014) · Microbiology · doi:10.1099/mic.0.000009 · Cited by 36 · see citing works
Self-citationde Queiroz, T. B., dos Santos, G. F., Ventura, S. C., et al. (2017) · Genetics and Molecular Research · doi:10.4238/gmr16039777 · Cited by 35 · see citing works
Ramos, A. A., Pedro, D. F. N., Collins, A. R., & Pereira‐Wilson, C. (2012) · Journal of Toxicology and Environmental Health · doi:10.1080/15287394.2012.689804 · Cited by 32 · see citing works
Habibi, E., Shokrzadeh, M., Ahmadi, A., et al. (2014) · Pharmaceutical Biology · doi:10.3109/13880209.2014.910674 · Cited by 29 · see citing works
Llana‐Ruíz‐Cabello, M., Maisanaba, S., Gutiérrez‐Praena, D., et al. (2014) · Food Chemistry · doi:10.1016/j.foodchem.2014.06.058 · Cited by 27 · see citing works
Cytotoxic, anti-carcinogenic and antioxidant properties of the most frequent plant volatiles
Slameňová, D., & Horváthová, E. (2013) · Neoplasma · doi:10.4149/neo_2013_046 · Cited by 27 · see citing works
Ramos, A. A., Marques, F., Fernandes‐Ferreira, M., et al. (2012) · Food and Chemical Toxicology · doi:10.1016/j.fct.2012.09.014 · Cited by 24 · see citing works
Mellado-García, P., Maisanaba, S., Puerto, M., et al. (2015) · Food and Chemical Toxicology · doi:10.1016/j.fct.2015.11.011 · Cited by 24 · see citing works
Methyleugenol and oxidative metabolites induce DNA damage and interact with human topoisomerases
Groh, I. A. M., Rudakovski, O., Gründken, M., Schroeter, A., Marko, D., & Esselen, M. (2015) · Archives of Toxicology · doi:10.1007/s00204-015-1625-3 · Cited by 20 · see citing works
Recent Perspectives on the Relations between Fecal Mutagenicity, Genotoxicity, and Diet
Gratz, S. W., Wallace, R. J., & El-Nezami, H. S. (2011) · Frontiers in Pharmacology · doi:10.3389/fphar.2011.00004 · Cited by 20 · see citing works
Self-citationPEPPERMINT OIL AND IRRITABLE BOWEL SYNDROME
Koch, T. R. (1998) · The American Journal of Gastroenterology · doi:10.1111/j.1572-0241.1998.02304.x · Cited by 12 · see citing works
McDougall, G. J., Allwood, J. W., Pereira‐Caro, G., Brown, E. M., Latimer, C., Dobson, G., Stewart, D., Ternan, N. G., Lawther, R., O’Connor, G., et al. (2017) · Food & Function · doi:10.1039/c7fo00846e · Cited by 12 · see citing works
Thirugnanasampandan, R., Ramya, G., Gogulramnath, M., et al. (2015) · Pharmacognosy Journal · doi:10.5530/pj.2015.1.3 · Cited by 7 · see citing works
Use of Single-cell Gel Electrophoresis Assays in Dietary Intervention Trials
Nersesyan, A. K., Hoelzl, C., Ferk, F., Mišík, M., Al-Serori, H., Setayesh, T., & Knasmüller, S. (2016) · Royal Society of Chemistry eBooks · doi:10.1039/9781782622895-00314 · Cited by 3 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Haro-González, J. N., Castillo-Herrera, G. A., Martínez-Velázquez, M., & Espinosa-Andrews, H. (2021) · Molecules · doi:10.3390/molecules26216387 · Cited by 463 · see citing works
- Authors
- Thapa, D., Richardson, A. J., Zweifel, B., Wallace, R. J., & Gratz, S. W.
- Affiliations
- Teagasc - The Irish Agriculture and Food Development Authority (IE)
- University of Aberdeen (GB)
- Agolin SA 1145 Bière Switzerland
- Corresponding author
- Gratz, Silvia W.
- Venue
- Journal of Food Science
- Publisher
- Wiley
- Published
- 2019-06-17
- Volume
- 84
- Issue
- 7
- Pages
- 1979-1985
- ISSN
- 0022-1147 (ISSN-L)
- 1750-3841
- OpenAlex
- W2952071840
- Cited by
- 39
- References
- 56
- Citations by year
- 2026: 4 · 2025: 7 · 2024: 8 · 2023: 6 · 2022: 3 · 2021: 8 · 2020: 3
- FWCI
- 1.10
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Food Science (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Rural and Environment Science and Analytical Services Division (RESAS)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Carcinogens and Genotoxicity Assessment
- Life Sciences › Agricultural and Biological Sciences › Food Science › Essential Oils and Antimicrobial Activity
- Physical Sciences › Chemistry › Organic Chemistry › Edible Oils Quality and Analysis
- Keywords
- essential oil constituents, genoprotective effect, oxidative DNA damage, thymol, geraniol, nerolidol, linalool, eugenol, HT-29 cells, colorectal cancer, colonic epithelium, cytotoxicity, genotoxicity, nutraceuticals
- MeSH terms
- Acyclic Monoterpenes, Colonic Neoplasms, DNA Damage, Eugenol, Humans, Oils, Volatile, Terpenes, Thymol, Oxidative Stress, DNA Methylation, Protective Agents, Comet Assay, Monoterpenes, Cell Line, Tumor
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
