Decellularization and Recellularization of Rat Livers With Hepatocytes and Endothelial Progenitor Cells
Abstract
Whole‐organ decellularization has been identified as a promising choice for tissue engineering. The aim of the present study was to engineer intact whole rat liver scaffolds and repopulate them with hepatocytes and endothelial progenitor cells (EPCs) in a bioreactor. Decellularized liver scaffolds were obtained by perfusing T riton X‐100 with ammonium hydroxide. The architecture and composition of the original extracellular matrix were preserved, as confirmed by morphologic, histological, and immunolabeling methods. To determine biocompatibility, the scaffold was embedded in the subcutaneous adipose layer of the back of a heterologous animal to observe the infiltration of inflammatory cells. Hepatocytes were reseeded using a parenchymal injection method and cultured by continuous perfusion. EPCs were reseeded using a portal vein infusion method. Morphologic and functional examination showed that the hepatocytes and EPCs grew well in the scaffold. The present study describes an effective method of decellularization and recellularization of rat livers, providing the foundation for liver engineering and the development of bioartificial livers.
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The difference in engraftment was attributed to the tendency of reseeded cells to block the portal vein lumen and be washed out of the scaffolds.
“This difference was attributed to the tendency of reseeded cells to block the portal vein lumen and be washed out of the scaffolds.”
From Liver tissue engineering using decellularized scaffolds: Current progress, challenges, and opportunities (2024) · cites this paper as [105]
Zhou et al. found that cells delivered via portal vein infusion resulted in a low engraftment rate (83.4% ± 5.2%) and poor distribution, whereas parenchymal injection resulted in a high engraftment rate (93.2% ± 4%) and better distribution of cells in the parenchymal space after 24 h.
“Cells delivered via portal vein infusion resulted in a low engraftment rate (83.4 % ± 5.2 %) and poor distribution of the seeding cells, whereas parenchymal injection resulted in a high engraftment rate (93.2 % ± 4 %) and better distribution of cells in the parenchymal space after 24 h.”
From Liver tissue engineering using decellularized scaffolds: Current progress, challenges, and opportunities (2024) · cites this paper as [105]
EPCs isolated from rat bone marrow and infused into decellularized rat livers through the portal vein covered the vascular structures within the decellularized liver after 3 days of media perfusion.
“Zhou et al. infused decellularized rat livers through the portal vein with EPCs isolated from rat bone marrow. The reseeded cells covered the vascular structures within the decellularized liver after 3 days of media perfusion [105].”
From Liver tissue engineering using decellularized scaffolds: Current progress, challenges, and opportunities (2024) · cites this paper as [105]
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“Zhou et al. conducted a study comparing the repopulation efficiency of buffalo rat liver (BRL) epithelial-like cells after intraparenchymal injection or portal vein infusion [105].”
Background
- Authors
- Zhou, P., Huang, Y., Guo, Y., Wang, L., Ling, C., Guo, Q., Wang, Y., Zhu, S., Fan, X., Zhu, M., Huang, H., Lu, Y., & Wang, Z.
- Affiliations
- Affiliated Hospital of Nantong University (CN)
- Department of General Surgery Affiliated Hospital of Nantong University Nantong China
- Corresponding author
- Wang, Zhiwei
- ORCID
- Venue
- Artificial Organs
- Publisher
- Wiley
- Published
- 2015-12-04
- Volume
- 40
- Issue
- 3
- Pages
- E25-38
- ISSN
- 0160-564X (ISSN-L)
- 1525-1594
- OpenAlex
- W2187990222
- Cited by
- 73
- References
- 7
- Citations by year
- 2026: 1 · 2025: 5 · 2024: 5 · 2023: 8 · 2022: 5 · 2021: 12 · 2020: 5 · 2019: 9 · 2018: 10 · 2017: 11 · 2016: 2
- FWCI
- 7.73
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Artificial Organs (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Natural Science Foundation of China (81471801)
- Topics
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Health Sciences › Medicine › Hepatology › Liver physiology and pathology
- Keywords
- hepatocytes, endothelial progenitor cells, extracellular matrix preservation, Triton X-100, ammonium hydroxide, portal vein infusion, heterotopic implantation, bioartificial liver, liver tissue engineering
- MeSH terms
- Animals, Cells, Cultured, Extracellular Matrix, Liver, Mice, Inbred C57BL, Rats, Sprague-Dawley, Bioreactors, Hepatocytes, Tissue Engineering, Cell Proliferation, Rats, Tissue Scaffolds, Endothelial Progenitor Cells
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