Decellularization methods of porcine kidneys for whole organ engineering using a high-throughput system
Abstract
End-stage renal failure is a devastating disease, with donor organ transplantation as the only functional restorative treatment. The current number of donor organs meets less than one-fifth of demand, so regenerative medicine approaches have been proposed as potential therapeutic alternatives. One such approach for whole large-organ bioengineering is to combine functional renal cells with a decellularized porcine kidney scaffold. The efficacy of cellular removal and biocompatibility of the preserved porcine matrices, as well as scaffold reproducibility, are critical to the success of this approach. We evaluated the effectiveness of 0.25 and 0.5% sodium dodecyl sulfate (SDS) and 1% Triton X-100 in the decellularization of adult porcine kidneys. To perform the decellularization, a high-throughput system was designed and constructed. In this study all three methods examined showed significant cellular removal, but 0.5% SDS was the most effective detergent (<50 ng DNA/mg dry tissue). Decellularized organs retained intact microarchitecture including the renal vasculature and essential extracellular matrix components. The SDS-treated decellularized scaffolds were non-cytotoxic to primary human renal cells. This method ensures clearance of porcine cellular material (which directly impacts immunoreactivity during transplantation) and preserves the extracellular matrix and cellular compatibility of these renal scaffolds. Thus, we have developed a rapid decellularization method that can be scaled up for use in other large organs, and this represents a step toward development of a transplantable organ using tissue engineering techniques.
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Although chemical agents play a key role in decellularization and removal of immunogenic materials, they may cause ECM damage and affect biocompatibility of yielded decellularized tissues.
“Although chemical agents play a key role in the decellularization and removal of the immunogenic materials from the decellularized tissues, they may cause ECM damage and affect the biocompatibility of the yielded decellularized tissues [5,7,[90], [91], [92]].”
From Liver tissue engineering using decellularized scaffolds: Current progress, challenges, and opportunities (2024) · cites this paper as [90]
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- Authors
- Sullivan, D. C., Mirmalek-Sani, S.-H., Deegan, D. B., Baptista, P. M., Aboushwareb, T., Atala, A., & Yoo, J. J.
- Affiliations
- Virginia Tech - Wake Forest University School of Biomedical Engineering & Sciences (US)
- Wake Forest University Health Sciences (US)
- Corresponding author
- Yoo, James J.
- Venue
- Biomaterials
- Publisher
- Elsevier BV
- Published
- 2012-11
- Volume
- 33
- Issue
- 31
- Pages
- 7756-7764
- ISSN
- 0142-9612 (ISSN-L)
- OpenAlex
- W1967540288
- Cited by
- 363
- References
- 59
- Citations by year
- 2026: 7 · 2025: 14 · 2024: 15 · 2023: 17 · 2022: 18 · 2021: 43 · 2020: 28 · 2019: 23 · 2018: 30 · 2017: 33 · 2016: 28 · 2015: 42 · 2014: 38 · 2013: 23 · 2012: 2
- FWCI
- 34.22
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- Locations
- Biomaterials (publishedVersion)
- PubMed (publishedVersion)
- Funders
- U.S. Department of Defense (W81XWH-07-1-0718)
- Topics
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Medicine › Hydrogels: synthesis, properties, applications
- Keywords
- decellularization, porcine kidney, sodium dodecyl sulfate, Triton X-100, extracellular matrix preservation, renal vasculature, biocompatibility, end-stage renal disease, organ transplantation, renal cell culture
- MeSH terms
- Animals, Cells, Cultured, Detergents, Extracellular Matrix, Humans, Kidney, Perfusion, Cell Death, Tissue Engineering, Sus scrofa, Tissue Scaffolds, High-Throughput Screening Assays
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