Inactivation of viruses in platelet concentrates by photochemical treatment with amotosalen and long‐wavelength ultraviolet light
Open accessAbstract
BACKGROUND: Viral contamination of platelet (PLT) concentrates can result in transfusion‐transmitted diseases. A photochemical treatment (PCT) process with amotosalen‐HCl and long‐wavelength ultraviolet light (UVA), which cross‐links nucleic acids, was developed to inactivate viruses and other pathogens in PLT concentrates. STUDY DESIGN AND METHODS: High titers of pathogenic or blood‐borne viruses, representing 10 different families, were added to single‐donor PLT concentrates containing 3.0 × 10 11 to 6.0 × 10 11 PLTs in approximately 300 mL of 35 percent plasma and 65 percent PLT additive solution (InterSol). After PCT with 150 µmol per L amotosalen and 3 J per cm 2 UVA, residual viral infectivity was assayed by sensitive cell culture or animal systems. RESULTS: Enveloped viruses were uniformly sensitive to inactivation by PCT whereas nonenveloped viruses demonstrated variable inactivation. Log reduction of enveloped viruses for cell‐free HIV‐1 was >6.2; for cell‐associated HIV‐1, >6.1; for clinical isolate HIV‐1, >3.4; for clinical isolate HIV‐2, >2.5; for HBV, >5.5; for HCV, >4.5; for DHBV, >6.2; for BVDV, >6.0; for HTLV‐I, 4.2; for HTLV‐II, 4.6; for CMV, >5.9; for WNV, >5.5; for SARS‐HCoV, >5.8; and for vaccinia virus, >4.7. Log reduction of nonenveloped viruses for human adenovirus 5 was >5.2; for parvovirus B19, 3.5‐>5.0; for bluetongue virus, 5.6‐5.9; for feline conjunctivitis virus, 1.7‐2.4; and for simian adenovirus 15, 0.7‐2.3. CONCLUSION: PCT inactivates a broad spectrum of pathogenic, blood‐borne viruses. Inactivation of viruses in PLT concentrates with amotosalen and UVA offers the potential to prospectively prevent the majority of PLT transfusion‐associated viral diseases.
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Psoralen derivatives are used in photopheresis of cutaneous T-cell lymphoma and photoinactivation of pathogens in plasma and platelet components.
“Other applications of psoralen derivatives deal with the photopheresis of cutaneous T-cell lymphoma (156-158) and the photoinactivation of pathogens in plasma and platelet components (159,160).”
From Photoinduced Damage to Cellular DNA: Direct and Photosensitized Reactions<sup>†</sup> (2012) · cites this paper as [159]
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Flagged first, then by citations
Research works printed but not linked: 3
- Lin L, Dikeman R, Molini B, et al. Photochemical treatment of platelet concentrates with amotosalen and UVA inactivates a broad spectrum of pathogenic bacteria. Transfusion 2004;44:1496-504.
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Photoinduced Damage to Cellular DNA: Direct and Photosensitized Reactions<sup>†</sup>
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Self-citation
- Authors
- Lin, L., Hanson, C. V., Alter, H. J., Jauvin, V., Bernard, K. A., Murthy, K. K., Metzel, P., & Corash, L.
- Affiliations
- Texas Biomedical Research Institute (US)
- Corresponding author
- Lin, Lily
- Venue
- Transfusion
- Publisher
- Wiley
- Published
- 2005-03-22
- Volume
- 45
- Issue
- 4
- Pages
- 580-590
- ISSN
- 0041-1132 (ISSN-L)
- 1537-2995
- PMC
- PMC7202329
- OpenAlex
- W2162642191
- Cited by
- 172
- References
- 57
- Citations by year
- 2026: 1 · 2025: 3 · 2024: 1 · 2023: 4 · 2022: 9 · 2021: 6 · 2020: 13 · 2019: 5 · 2018: 7 · 2017: 8 · 2016: 8 · 2015: 9 · 2014: 10 · 2013: 7 · 2012: 9
- FWCI
- 8.79
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Transfusion (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Cerus Corporation
- Baxter Healthcare Corporation
- U.S. Army Medical Research Acquisition Activity
- Topics
- Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Photodynamic Therapy Research Studies
- Health Sciences › Medicine › Biochemistry › Blood transfusion and management
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Microbial Inactivation Methods
- Keywords
- virus inactivation, photochemical treatment, amotosalen, UVA, transfusion-transmissible infections, HIV-1, hepatitis B virus, hepatitis C virus, non-enveloped viruses, enveloped viruses, blood-borne viruses
- MeSH terms
- Blood Banking, Humans, Furocoumarins, Ultraviolet Rays, Virus Diseases, Virus Replication, Viruses, Platelet Transfusion, In Vitro Techniques
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