The extent of amotosalen photodegradation during photochemical treatment of platelet components correlates with the level of pathogen inactivation
Abstract
BACKGROUND: Amotosalen plus ultraviolet A (UVA) light inactivates a broad range of bacteria, viruses, protozoa, and leukocytes in platelet (PLT) and plasma components. Upon UVA illumination a small fraction of amotosalen reacts with the nucleic acid of contaminating pathogens and residual white blood cells and the remaining fraction undergoes photodegradation into defined photoproducts. The levels of amotosalen and photoproducts can be accurately quantified. STUDY DESIGN AND METHODS: This study evaluated the relationship between the extent of photodegradation of amotosalen and the level of pathogen inactivation in PLT components. PLT components containing of 3.78 × 10 11 to 7.23 × 10 11 PLTs in 300 to 450 mL of 35% to 50% plasma and 50% to 65% PLT additive solution and up to 5 × 10 6 red blood cells (RBCs)/mL were prepared. Each component was contaminated with 10 5 to 10 6 colony‐forming units/mL Klebsiella pneumoniae and treated with 115 to 200 µmol/L amotosalen and 0 to 3 J/cm 2 UVA light. For each treatment condition, the level of K. pneumoniae inactivation (log‐reduction) was measured by microbiologic methods. The initial and postillumination amotosalen concentrations (µmol/L) were determined by high‐performance liquid chromatography. RESULTS: For a defined set of treatment conditions, the extent of amotosalen photodegradation was consistent and reproducible. The bacterial log‐reduction correlated linearly with the extent of amotosalen photodegradation with a regression correlation coefficient (r 2) between 0.845 and 0.890 regardless of the treatment variables such as PLT content, component volume, plasma content, RBC content, initial amotosalen concentration, and UVA dose. CONCLUSION: This study demonstrated that the extent of amotosalen photodegradation can serve as an intrinsic actinometer which directly correlated with the level of pathogen inactivation.
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Psoralen derivatives are used in photopheresis of cutaneous T-cell lymphoma and photoinactivation of pathogens in plasma and platelet components.
“Other applications of psoralen derivatives deal with the photopheresis of cutaneous T-cell lymphoma (156-158) and the photoinactivation of pathogens in plasma and platelet components (159,160).”
From Photoinduced Damage to Cellular DNA: Direct and Photosensitized Reactions<sup>†</sup> (2012) · cites this paper as [160]
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Photoinduced Damage to Cellular DNA: Direct and Photosensitized Reactions<sup>†</sup>
Cadet, J., Mouret, S., Ravanat, J., & Douki, T. (2012) · Photochemistry and Photobiology · doi:10.1111/j.1751-1097.2012.01200.x · Cited by 300 · see citing works
- Authors
- Liu, W., Cimino, G. D., Corash, L., & Lin, L.
- Affiliations
- Cerus (United States) (US)
- Corresponding author
- Liu, Weiqun
- ORCID
- Corash, Laurence
- Venue
- Transfusion
- Publisher
- Wiley
- Published
- 2011-01
- Volume
- 51
- Issue
- 1
- Pages
- 52-61
- ISSN
- 0041-1132 (ISSN-L)
- 1537-2995
- OpenAlex
- W1944570428
- Cited by
- 12
- References
- 14
- Citations by year
- 2023: 1 · 2020: 1 · 2018: 1 · 2016: 1 · 2015: 1 · 2014: 2 · 2013: 1 · 2012: 2
- FWCI
- 1.33
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Transfusion (publishedVersion)
- PubMed (publishedVersion)
- Funders
- California Department of Public Health
- Topics
- Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Photodynamic Therapy Research Studies
- Health Sciences › Medicine › Biochemistry › Blood transfusion and management
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Microbial Inactivation Methods
- Keywords
- amotosalen, photodegradation, pathogen inactivation, platelet components, UVA irradiation, Klebsiella pneumoniae, actinometer, photoproducts, residual leukocytes
- MeSH terms
- Blood Platelets, Erythrocytes, Humans, Klebsiella pneumoniae, Photolysis, Furocoumarins, Stem Cells, Ultraviolet Rays
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