The DOI matches its Crossref record; 28 of the 28 references it cites were found in a registry or an index.
Cholesterol removal by some lactic acid bacteria that can be used as probiotic
Abstract
In the present study, the relationship between exopolysaccharide production and cholesterol removal rates of five strains of Lactobacillus delbrueckii subsp. bulgaricus isolated from home-made yoghurt was studied. Test strains were selected according to their exopolysaccharide production capacity. Influence of different bile concentrations on cholesterol removal was investigated. It was confirmed that B3, ATCC 11842 and G11 strains which produce high amounts of exopolysaccharide (211, 200 and 159 mg/l, respectively) were able to remove more cholesterol from the medium compared to those that produce low amounts of exopolysaccharide (B2, A13). The highest cholesterol removal (31%) was observed by strain L. delbrueckii subsp. bulgaricus B3, producing a high amount of exopolysaccharide, in 3 mg/ml bile concentration. Cholesterol removal by resting and dead cells was investigated and it was found to be 4%-14% and 3%-10%, respectively. Cholesterol removal by immobilized and free cells of the B3 strain was studied and it was determined that immobilized cells are more effective. Influence of cholesterol on exopolysaccharide production has also been tested and it was found that cholesterol increased the production of EPS. The results indicated that: (i) there is a correlation between cholesterol removal and EPS production; and (ii) L. delbrueckii subsp. bulgaricus B3 is regarded as a suitable candidate probiotic and adjunct culture.
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In the citing paper
“A study by Tok and Aslim [131] suggested that high EPS-producing Lb. delbrueckii ssp. bulgaricus strains were more potent to sequester cholesterol from the medium in vitro compared to low EPS producer strains.”
From Lactic Acid Bacteria Exopolysaccharides Producers: A Sustainable Tool for Functional Foods (2021) · cites this paper as [131]
In the citing paper
“Cardiovascular disease (CVD) is one of the leading causes of death in Western societies, and hypertension and hypercholesterolemia are recognized as the two major risk factors [131].”
From Lactic Acid Bacteria Exopolysaccharides Producers: A Sustainable Tool for Functional Foods (2021) · cites this paper as [131]
Citations
Cites
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An overview of the functionality of exopolysaccharides produced by lactic acid bacteria
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Acid and Bile Tolerance and Cholesterol Removal Ability of Lactobacilli Strains
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Exopolysaccharides from lactic acid bacteria: perspectives and challenges
Welman, A. D., & Maddox, I. S. (2003) · Trends in biotechnology · doi:10.1016/s0167-7799(03)00107-0 · Cited by 523
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High-Density Lipoprotein Restores Endothelial Function in Hypercholesterolemic Men
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Metadata
- Authors
- Tok, E., & Aslim, B.
- Affiliations
- Türkiye Bilimsel ve Teknolojik Araştırma Kurumu (TR)
- Venue
- Microbiology and Immunology
- Publisher
- Wiley
- Published
- 2010-03
- Volume
- 54
- Issue
- 5
- Pages
- 257-64
- ISSN
- 0385-5600 (ISSN-L)
- 1348-0421
- OpenAlex
- W1968457680
- Cited by
- 117
- References
- 28
- Citations by year
- 2026: 2 · 2025: 4 · 2024: 4 · 2023: 7 · 2022: 13 · 2021: 17 · 2020: 9 · 2019: 9 · 2018: 9 · 2017: 6 · 2016: 8 · 2015: 6 · 2014: 9 · 2013: 7 · 2012: 5
- FWCI
- 6.88
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Microbiology and Immunology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Life Sciences › Agricultural and Biological Sciences › Food Science › Polysaccharides Composition and Applications
- Life Sciences › Immunology and Microbiology › Microbiology › Reproductive tract infections research
- Keywords
- cholesterol removal, Lactobacillus bulgaricus, exopolysaccharide production, bile tolerance, probiotics, immobilized cells, dead cells, yogurt starter cultures, hypercholesterolemia
- MeSH terms
- Bile, Cholesterol, Food Microbiology, Polysaccharides, Bacterial, Yogurt, Colony Count, Microbial, Probiotics, Lactobacillus delbrueckii
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