Transferrin receptor expression on AML blasts is related to their proliferative potential
Abstract
Expression of the transferrin receptor (TfR) was studied on peripheral blood blast cells from 11 patients with acute myeloid leukaemia (AML). Using a monoclonal anti-TfR antibody (OKT9) and a polyclonal antibody against surface membrane-bound transferrin, a proportion of blasts from all the patients was found to express receptors for transferrin. Further analysis of OKT9 expression using a fluorescent activated cell sorter (FACS) showed that the TfR was heterogeneously distributed in the blast cell population. In five out of six samples studied, stimulation of DNA synthesis following short-term culture induced a several-fold increase in TfR display as analysed by flow cytometry using OKT9 or FITC-conjugated transferrin. Blasts from seven patients stained with OKT9 were separated on the FACS into positive and negative or weakly positive fractions. Culture of the TfR negative population in a blast cell colony assay produced no colonies in either of two patients. In a further five patients the colony forming cells were predominantly associated with the TfR strongly positive fraction (52 +/- 25 colonies/10(4) cells) rather than the TfR weakly positive fraction (12 +/- 11 colonies/10(4) cells). Analysis of colony size showed that clones derived from the weakly positive fraction were smaller than clones from the TfR strongly positive fraction. These results suggest that TfR display by AML blasts is related to their proliferative capacity and is expressed by the leukaemic stem cell fraction.
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Anti-TfR treatment was only effective in some cancers such as adult T-cell leukemia/lymphoma (ATL) and leukemia.
“However, treatment was only effective in some cancers such as adult T-cell leukemia/lymphoma (ATL) and leukemia [99,100].”
From Iron as a central player and promising target in cancer progression (2019) · cites this paper as [99]
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Iron as a central player and promising target in cancer progression
Jung, M., Mertens, C., Tomat, E., & Brüne, B. (2019) · International Journal of Molecular Sciences · doi:10.3390/ijms20020273 · Cited by 268 · see citing works
“However, treatment was only effective in some cancers such as adult T-cell leukemia/lymphoma (ATL) and leukemia [99,100].”
Background
- Authors
- Kozlowski, R., Reilly, I. A. G., Sowter, D., Robins, R. A., & Russell, N. H.
- Affiliations
- Nottingham City Hospital (GB)
- University of Nottingham (GB)
- Corresponding author
- Russell, N. H.
- ORCID
- Russell, N. H.
- Venue
- British Journal of Haematology
- Publisher
- Wiley
- Published
- 1988-06
- Volume
- 69
- Issue
- 2
- Pages
- 275-280
- ISSN
- 0007-1048 (ISSN-L)
- 1365-2141
- OpenAlex
- W2046219922
- Cited by
- 18
- References
- 30
- Citations by year
- 2022: 1 · 2019: 1
- FWCI
- 1.71
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- British Journal of Haematology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Hematology › Acute Myeloid Leukemia Research
- Health Sciences › Medicine › Hematology › Iron Metabolism and Disorders
- Health Sciences › Medicine › Hematology › Hematopoietic Stem Cell Transplantation
- Keywords
- transferrin receptor, acute myeloid leukemia, leukemia stem cells, OKT9, transferrin, colony formation, cell surface receptors
- MeSH terms
- Antibodies, Monoclonal, Cell Division, Fluorescent Antibody Technique, Humans, Receptors, Transferrin, Tumor Stem Cell Assay, Leukemia, Myeloid, Acute
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