The DOI matches its Crossref record; 163 of the 163 references it cites were found in a registry or an index.
A critical analysis of current in vitro and in vivo angiogenesis assays
Open accessAbstract
Summary: The study of angiogenesis has grown exponentially over the past 40 years with the recognition that angiogenesis is essential for numerous pathologies and, more recently, with the advent of successful drugs to inhibit angiogenesis in tumours. The main problem with angiogenesis research remains the choice of appropriate assays to evaluate the efficacy of potential new drugs and to identify potential targets within the angiogenic process. This selection is made more complex by the recognition that heterogeneity occurs, not only within the endothelial cells themselves, but also within the specific microenvironment to be studied. Thus, it is essential to choose the assay conditions and cell types that most closely resemble the angiogenic disease being studied. This is especially important when aiming to translate data from in vitro to in vivo and from preclinical to the clinic. Here we critically review and highlight recent advances in the principle assays in common use including those for endothelial cell proliferation, migration, differentiation and co‐culture with fibroblasts and mural cells in vitro, vessel outgrowth from organ cultures and in vivo assays such as chick chorioallantoic membrane (CAM), zebrafish, sponge implantation, corneal, dorsal air sac, chamber and tumour angiogenesis models. Finally, we briefly discuss the direction likely to be taken in future studies, which include the use of increasingly sophisticated imaging analysis systems for data acquisition.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Angiogenesis in cancer and other diseases
Carmeliet, P., & Jain, R. K. (2000) · Nature · doi:10.1038/35025220 · Cited by 9,070
Jaffe, E. A., Nachman, R. L., Becker, C. G., & Minick, C. R. (1973) · Journal of Clinical Investigation · doi:10.1172/jci107470 · Cited by 6,856
Rapid colorimetric assay for cell growth and survival
Denizot, F., & Lang, R. (1986) · Journal of Immunological Methods · doi:10.1016/0022-1759(86)90368-6 · Cited by 4,935
THE CHEMOTACTIC EFFECT OF MIXTURES OF ANTIBODY AND ANTIGEN ON POLYMORPHONUCLEAR LEUCOCYTES
Boyden, S. (1962) · The Journal of Experimental Medicine · doi:10.1084/jem.115.3.453 · Cited by 2,733
‘Green mice’ as a source of ubiquitous green cells
Okabe, M., Ikawa, M., Kominami, K., Nakanishi, T., & Nishimune, Y. (1997) · FEBS Letters · doi:10.1016/s0014-5793(97)00313-x · Cited by 2,581
Thalidomide is an inhibitor of angiogenesis.
D'Amato, R. J., Loughnan, M. S., Flynn, E., & Folkman, J. (1994) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.91.9.4082 · Cited by 2,454
Hanahan, D. (1985) · Nature · doi:10.1038/315115a0 · Cited by 1,281
HMEC-1: Establishment of an Immortalized Human Microvascular Endothelial Cell Line
Ades, E. W., Candal, F. J., Swerlick, R. A., George, V. G., Summers, S., Bosse, D. C., & Lawley, T. J. (1992) · Journal of Investigative Dermatology · doi:10.1111/1523-1747.ep12613748 · Cited by 1,265
Macrophage-induced angiogenesis is mediated by tumour necrosis factor-α
Leibovich, S. J., Polverini, P. J., Shepard, H. M., Wiseman, D. M., Shively, V., & Nuseir, N. (1987) · Nature · doi:10.1038/329630a0 · Cited by 1,210
Lin, E. Y., Jones, J. G., Li, P., Zhu, L., Whitney, K. D., Muller, W. J., & Pollard, J. W. (2003) · The American Journal of Pathology · doi:10.1016/s0002-9440(10)63568-7 · Cited by 1,107
Cited by
Nanocomposite scaffolds for accelerating chronic wound healing by enhancing angiogenesis
Nosrati, H., Aramideh Khouy, R., Nosrati, A., Khodaei, M., Banitalebi-Dehkordi, M., Ashrafi-Dehkordi, K., Sanami, S., & Alizadeh, Z. (2021) · Journal of Nanobiotechnology · doi:10.1186/s12951-020-00755-7 · Cited by 720
Organotypic brain slice cultures: A review
Humpel, C. (2015) · Neuroscience · doi:10.1016/j.neuroscience.2015.07.086 · Cited by 480
Kebschull, M., Demmer, R. T., & Papapanou, P. N. (2010) · Journal of Dental Research · doi:10.1177/0022034510375281 · Cited by 429
Hydrogels to model 3D in vitro microenvironment of tumor vascularization
Song, H.-H. G., Park, K. M., & Gerecht, S. (2014) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2014.06.002 · Cited by 153
Belair, D. G., Whisler, J. A., Valdez, J., Velazquez, J., Molenda, J. A., Vickerman, V., Lewis, R., Daigh, C., Hansen, T. D., Mann, D. A., et al. (2015) · Stem Cell Reviews and Reports · doi:10.1007/s12015-014-9549-5 · Cited by 120
Metadata
- Authors
- Staton, C. A., Reed, M. W. R., & Brown, N. J.
- Venue
- International Journal of Experimental Pathology
- Publisher
- Wiley
- Published
- 2009-05-11
- Volume
- 90
- Issue
- 3
- Pages
- 195-221
- ISSN
- 0959-9673 (ISSN-L)
- 1365-2613
- OpenAlex
- W1551620887
- Cited by
- 469
- References
- 163
- FWCI
- 8.16
- Type
- journal article
- Language
- en
- Open access
- green
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: A critical analysis of current in vitro and in vivo angiogenesis assays. https://app.cochat.ai/papers/10.1111/j.1365-2613.2008.00633.x
- The paper
- Staton, C. A., Reed, M. W. R., & Brown, N. J. (2009). A critical analysis of current in vitro and in vivo angiogenesis assays. International Journal of Experimental Pathology. https://doi.org/10.1111/j.1365-2613.2008.00633.x
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
