Cited by 133Algal biology and biofuel productionMicrobial Metabolic Engineering and BioproductionPlant biochemistry and biosynthesis

Increased synthesis of α-tocopherol, paramylon and tyrosine by Euglena gracilis under conditions of high biomass production: Metabolites biosynthesis by E. gracilis

Rodríguez-Zavala, J.S., Ortiz-Cruz, M.A., Mendoza-Hernández, G., Moreno-Sánchez, R.·2010·Journal of Applied Microbiology

Abstract

AIMS: To analyse the production of different metabolites by dark-grown Euglena gracilis under conditions found to render high cell growth. METHODS AND RESULTS: The combination of glutamate (5 g l(-1) ), malate (2 g l(-1) ) and ethanol (10 ml l(-1) ) (GM + EtOH); glutamate (7·15 g l(-1) ) and ethanol (10 ml l(-1) ); or malate (8·16 g l(-1) ), glucose (10·6 g l(-1) ) and NH(4) Cl (1·8 g l(-1) ) as carbon and nitrogen sources, promoted an increase of 5·6, 3·7 and 2·6-fold, respectively, in biomass concentration in comparison with glutamate and malate (GM). In turn, the production of α-tocopherol after 120 h identified by LC-MS was 3·7 ± 0·2, 2·4 ± 0·1 and 2 ± 0·1 mg [g dry weight (DW)](-1) , respectively, while in the control medium (GM) it was 0·72 ± 0·1 mg (g DW)(-1) . For paramylon synthesis, the addition of EtOH or glucose induced a higher production. Amino acids were assayed by RP-HPLC; Tyr a tocopherol precursor and Ala an amino acid with antioxidant activity were the amino acids synthesized at higher concentration. CONCLUSIONS: Dark-grown E. gracilis Z is a suitable source for the generation of the biotechnologically relevant metabolites tyrosine, α-tocopherol and paramylon. SIGNIFICANCE AND IMPACT OF THE STUDY: By combining different carbon and nitrogen sources and inducing a tolerable stress to the cell by adding ethanol, it was possible to increase the production of biomass, paramylon, α-tocopherol and some amino acids. The concentrations of α-tocopherol achieved in this study are higher than others reported previously for Euglena, plant and algal systems. This work helps to understand the effect of different carbon sources on the synthesis of bio-molecules by E. gracilis and can be used as a basis for future works to improve the production of different metabolites of biotechnological importance by this organism.

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Crossref · 10.1111/j.1365-2672.2010.04848.x

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  • Euglena gracilis produces high concentrations of α-tocopherol (3.7 ± 0.2 mg/g).

    “Rodriguez-Zavala et al. (2010) identified a microalga Euglena gracilis for producing high concentrations of α-tocopherol (3.7 ± 0.2 mg/g).”

    From Microalgae Metabolites: A Rich Source for Food and Medicine (2019) · cites this paper as Rodriguez-Zavala et al. (2010)

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Authors
Rodríguez-Zavala, J. S., Ortiz-Cruz, M. A., Mendoza-Hernández, G., & Moreno-Sánchez, R.
Affiliations
Instituto Nacional de Cardiología (MX)
Venue
Journal of Applied Microbiology
Published
2010-09-21
Volume
109
Issue
6
Pages
2160-2172
ISSN
1364-5072 (ISSN-L)
OpenAlex
W1877883339
Cited by
133
References
89
Citations by year
2026: 12 · 2025: 5 · 2024: 10 · 2023: 6 · 2022: 8 · 2021: 16 · 2020: 9 · 2019: 13 · 2018: 10 · 2017: 13 · 2016: 10 · 2015: 9 · 2014: 5 · 2013: 2 · 2012: 2
FWCI
1.04
Type
journal article
Language
en
Open access
closed
Locations
  • Journal of Applied Microbiology (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Instituto de Ciencia y Tecnología del Distrito Federal
  • Consejo Nacional de Ciencia y Tecnología
Topics
  • Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant biochemistry and biosynthesis
Keywords
Euglena gracilis, alpha-tocopherol, paramylon, tyrosine, biomass production, carbon and nitrogen sources, ethanol stress, glutamate, malate, glucose, amino acid production, metabolite production
MeSH terms
Biotechnology, Culture Media, Euglena gracilis, Glucans, Glucose, Tyrosine, Biomass, alpha-Tocopherol

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