Cited by 177Algal biology and biofuel productionSeaweed-derived Bioactive CompoundsAquaculture Nutrition and Growth

Vitamin E (α-tocopherol) production by the marine microalgae Dunaliella tertiolecta and Tetraselmis suecica in batch cultivation

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Carballo-Cárdenas, Eira C., Tuan, Pham Minh, Janssen, Marcel, Wijffels, René H.·2003·Biomolecular Engineering

Abstract

Batch experiments with Dunaliella tertiolecta and Tetraselmis suecica were performed to investigate alpha-tocopherol (alpha-T) production in time, in order to assess the effect of light availability per cell on the production of this antioxidant. In D. tertiolecta alpha-T content increased during growth, in other words, as the cell density increased and the light availability per cell decreased. In T. suecica the pattern was different: alpha-T content was highest during the exponential phase, decreased significantly during the linear phase and increased again towards the end of the cultivation. Chlorophyll (chl-a) content of T. suecica cells decreased after the exponential phase, instead of the expected increase typically observed in shade-adapted cells, suggesting that the culture was nutrient limited. An experiment with extra nutrients showed that chl-a and alpha-T content increased significantly during both the linear and the stationary phase when compared with values in nutrient-deficient conditions. No correlation between alpha-T and chl-a was observed. Our results indicate that diminished light availability does not limit alpha-T production in D. tertiolecta and T. suecica, opening up the possibility of using high cell density, light-limited cultures for the production of this commercially interesting compound.

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Crossref · 10.1016/s1389-0344(03)00040-6

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Authors
Carballo-Cárdenas, E. C., Tuan, P. M., Janssen, M., & Wijffels, R. H.
Affiliations
Wageningen University & Research (NL)
Corresponding author
Carballo-Cárdenas, Eira C.
Venue
Biomolecular Engineering
Publisher
Elsevier BV
Published
2003-07
Volume
20
Issue
4-6
Pages
139-147
ISSN
1389-0344 (ISSN-L)
OpenAlex
W2075095946
Cited by
177
References
50
Citations by year
2026: 7 · 2025: 7 · 2024: 11 · 2023: 12 · 2022: 12 · 2021: 14 · 2020: 14 · 2019: 9 · 2018: 9 · 2017: 11 · 2016: 7 · 2015: 11 · 2014: 9 · 2013: 9 · 2012: 4
FWCI
1.14
Type
journal article
Language
en
Open access
Locations
  • Biomolecular Engineering (publishedVersion)
  • PubMed (publishedVersion)
  • VU Research Portal (publishedVersion)
  • Digital Academic REpository of VU University Amsterdam (Vrije Universiteit Amsterdam) (submittedVersion)
  • Socio-Environmental Systems Modeling (submittedVersion)
  • Data Archiving and Networked Services (DANS) (submittedVersion)
Topics
  • Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
  • Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Seaweed-derived Bioactive Compounds
  • Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Aquaculture Nutrition and Growth
Keywords
alpha-tocopherol, Dunaliella tertiolecta, Tetraselmis suecica, marine microalgae, batch culture, cell density, chlorophyll a, nutrient limitation, antioxidant production, microalgal biotechnology
MeSH terms
Chlorophyll A, Adaptation, Physiological, Chlorophyta, Cell Division, Cells, Cultured, Chlorophyll, Dose-Response Relationship, Radiation, Nitrates, Radiation Dosage, Species Specificity, Cell Culture Techniques, Bioreactors, alpha-Tocopherol

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