Vitamin E (α-tocopherol) production by the marine microalgae Dunaliella tertiolecta and Tetraselmis suecica in batch cultivation
Open accessAbstract
Batch experiments with Dunaliella tertiolecta and Tetraselmis suecica were performed to investigate alpha-tocopherol (alpha-T) production in time, in order to assess the effect of light availability per cell on the production of this antioxidant. In D. tertiolecta alpha-T content increased during growth, in other words, as the cell density increased and the light availability per cell decreased. In T. suecica the pattern was different: alpha-T content was highest during the exponential phase, decreased significantly during the linear phase and increased again towards the end of the cultivation. Chlorophyll (chl-a) content of T. suecica cells decreased after the exponential phase, instead of the expected increase typically observed in shade-adapted cells, suggesting that the culture was nutrient limited. An experiment with extra nutrients showed that chl-a and alpha-T content increased significantly during both the linear and the stationary phase when compared with values in nutrient-deficient conditions. No correlation between alpha-T and chl-a was observed. Our results indicate that diminished light availability does not limit alpha-T production in D. tertiolecta and T. suecica, opening up the possibility of using high cell density, light-limited cultures for the production of this commercially interesting compound.
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- Authors
- Carballo-Cárdenas, E. C., Tuan, P. M., Janssen, M., & Wijffels, R. H.
- Affiliations
- Wageningen University & Research (NL)
- Corresponding author
- Carballo-Cárdenas, Eira C.
- Venue
- Biomolecular Engineering
- Publisher
- Elsevier BV
- Published
- 2003-07
- Volume
- 20
- Issue
- 4-6
- Pages
- 139-147
- ISSN
- 1389-0344 (ISSN-L)
- OpenAlex
- W2075095946
- Cited by
- 177
- References
- 50
- Citations by year
- 2026: 7 · 2025: 7 · 2024: 11 · 2023: 12 · 2022: 12 · 2021: 14 · 2020: 14 · 2019: 9 · 2018: 9 · 2017: 11 · 2016: 7 · 2015: 11 · 2014: 9 · 2013: 9 · 2012: 4
- FWCI
- 1.14
- Type
- journal article
- Language
- en
- Open access
- green
- research.wur.nl
- Locations
- Biomolecular Engineering (publishedVersion)
- PubMed (publishedVersion)
- VU Research Portal (publishedVersion)
- Digital Academic REpository of VU University Amsterdam (Vrije Universiteit Amsterdam) (submittedVersion)
- Socio-Environmental Systems Modeling (submittedVersion)
- Data Archiving and Networked Services (DANS) (submittedVersion)
- Topics
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Seaweed-derived Bioactive Compounds
- Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Aquaculture Nutrition and Growth
- Keywords
- alpha-tocopherol, Dunaliella tertiolecta, Tetraselmis suecica, marine microalgae, batch culture, cell density, chlorophyll a, nutrient limitation, antioxidant production, microalgal biotechnology
- MeSH terms
- Chlorophyll A, Adaptation, Physiological, Chlorophyta, Cell Division, Cells, Cultured, Chlorophyll, Dose-Response Relationship, Radiation, Nitrates, Radiation Dosage, Species Specificity, Cell Culture Techniques, Bioreactors, alpha-Tocopherol
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