Cited by 142Escherichia coli research studiesBiochemical and Structural CharacterizationCell Adhesion Molecules Research

The O75X adhesin of uropathogenic Escherichia coli is a type IV collagen‐binding protein

Westerlund, B., Kuusela, P., Risteli, J., Risteli, L., Vartio, T., Rauvala, H., Virkola, R., Korhonen, T. K.·1989·Molecular Microbiology

Abstract

Summary: Interaction of the basement‐membrane binding O75X adhesin of uropathogenic Escherichia coli with various extracellular matrix proteins was studied. The adhesin showed strong binding to type IV collagen immobilized on microtitre plates, whereas other collagens, laminin and fibronectin, were only weakly recognized. Similarly, specific binding of [125 I]‐labelled type IV collagen to 075X‐positive bacteria was shown. Interaction of the two proteins was also demonstrated by affinity chromatography of the O75X adhesin on immobilized type IV collagen. The adhesin bound strongly to the immobilized N‐terminal 7S domain of type IV collagen, and the binding of [125 I]‐labelled type IV collagen to O75X‐positive bacteria was inhibited by the soluble 7S domain. Binding of O75X to type IV collagen and to its 7S domain was specifically inhibited by chloramphenicol but was not affected by periodate or endoglycosidase‐H treatment of the glycoproteins. Our results show that the 7S domain of type IV collagen is the basement membrane receptor for the O75X adhesin and suggest an interaction based on protein‐protein recognition. Inhibition of the interaction by chloramphenicol favours the supposition that a modified tyrosine is involved in the binding site.

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Crossref · 10.1111/j.1365-2958.1989.tb00178.x

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Authors
Westerlund, B., Kuusela, P., Risteli, J., Risteli, L., Vartio, T., Rauvala, H., Virkola, R., & Korhonen, T. K.
Affiliations
  • University of Oulu (FI)
  • University of Helsinki (FI)
Venue
Molecular Microbiology
Publisher
Wiley
Published
1989-03
Volume
3
Issue
3
Pages
329-337
ISSN
  • 0950-382X (ISSN-L)
  • 1365-2958
OpenAlex
W2075419819
Cited by
142
References
53
Citations by year
2026: 2 · 2025: 3 · 2022: 1 · 2021: 1 · 2020: 3 · 2019: 2 · 2018: 1 · 2016: 3 · 2015: 1 · 2014: 3 · 2013: 7 · 2012: 4
FWCI
5.68
Type
journal article
Language
en
Open access
closed
Locations
  • Molecular Microbiology (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Endocrinology › Escherichia coli research studies
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Biochemical and Structural Characterization
  • Health Sciences › Medicine › Immunology and Allergy › Cell Adhesion Molecules Research
Keywords
uropathogenic Escherichia coli, type IV collagen, bacterial adhesion, extracellular matrix proteins, laminin, fibronectin, chloramphenicol inhibition, protein-protein interaction
MeSH terms
Acetylglucosaminidase, Bacterial Adhesion, Bacterial Outer Membrane Proteins, Basement Membrane, Chloramphenicol, Chromatography, Affinity, Collagen, Escherichia coli, Fibronectins, Laminin, Periodic Acid, Protein Binding, Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase, Adhesins, Escherichia coli

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