Interaction of Dr Adhesin with Collagen Type IV Is a Critical Step in Escherichia coli Renal Persistence
Open accessAbstract
The pathogenic mechanism of recurrent or chronic urinary tract infection is poorly understood. Escherichia coli cells bearing Dr fimbriae display unique tropism to the basement membrane (BM)-renal interstitium that enables the bacteria to cause chronic pyelonephritis in experimental mice. The renal receptors for Dr-fimbriated E. coli are type IV collagen and decay-accelerating factor (DAF). We hypothesized that type IV collagen receptor-mediated BM-interstitial tropism is essential for E. coli to cause chronic pyelonephritis. To test the role of the type IV collagen tropism of Dr-fimbriated E. coli in renal persistence, we constructed an isogenic mutant in the DraE adhesin subunit that was unable to bind type IV collagen but retained binding to DAF and examined its virulence in the mouse model. The collagen-binding mutant DrI113T was eliminated from the mouse renal tissues in 6 to 8 weeks, while the parent strain caused persistent renal infection that lasted at least 14 weeks ( P ≤ 0.02). Transcomplementation with the intact Dr operon restored collagen-binding activity, BM-interstitial tropism, and the ability to cause persistent renal infection. We conclude that type IV collagen binding mediated by DraE adhesin is a critical step for the development of persistent renal infection in a murine model of E. coli pyelonephritis.
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In Escherichia coli, various adhesins from different fimbrial types facilitate binding to host ECM proteins, such as collagens and fibronectin, which contributes to its persistent colonization and infection.
“In Escherichia coli, various adhesins from different fimbrial types facilitate binding to host ECM proteins, such as collagens and fibronectin, which contributes to its persistent colonization and infection58–62.”
From Adhesin Als4112 promotes Candida auris skin colonization through interactions with keratinocytes and extracellular matrix proteins (2025) · cites this paper as [58]
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“Replacement of a single amino acid at the 113 position of DraE results in loss of the type IV collagenbinding property (Carnoy & Moseley, 1997; Selvarangan et al., 2004).”
BackgroundJung, J. H., Hong, H. J., Gharderpour, A., et al. (2019) · Scientific Reports · doi:10.1038/s41598-019-52070-3 · Cited by 7 · see citing works
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“Adhesins from the Dr/Afa family share a common receptor — DAF (Decay-Accelerat - ing Factor) originally responsible for protecting host tissues from complement system [21 ].”
Pathogenic Mechanisms of Uropathogens
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Asymptomatic Bacteriuria in Elderly Patients with Diabetes Attending a Tertiary Care Center
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Urinary Tract Infection. Why Do Some Children Get Complications, While Others Dont?
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Informing plasmid compatibility with bacterial hosts using protein-protein interaction data
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Mechanisms of Pathogenic Escherichia coli Attachment to Meat
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“They will also bind to type IV collagen (Selvarangan et al., 2004) primarily found in the skin within the basement of the membrane zone (Abreu-Velez and Howard, 2012).”
Diffusely Adhering Escherichia Coli
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“[74] on a mouse model demonstrated that DAEC binding to collagen is of vital importance in the pathomechanism of chronic glomerulonephritis.”
BackgroundUrinary tract infection and renal scarring
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To Move or to Convene : Regulatory Circuits of Mat Fimbriae in Escherichia coli
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Pathogenic Mechanisms of Uropathogens
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“In contrast to asymptomatic bacteriuria, UTI is a potentially life-threatening condition that is associated with host tissue damage resulting from bacterial virulence factors and mediators of the host immune response to infection (Lane & Mobley, 2007; Ong, Beatson, McEwan, & Schembri, 2009; Selvarangan et al., 2004; W…”
BackgroundInfluential
- Authors
- Selvarangan, R., Goluszko, P., Singhal, J., Carnoy, C., Moseley, S., Hudson, B., Nowicki, S., & Nowicki, B.
- Affiliations
- The University of Texas Medical Branch at Galveston (US)
- Institut de Biologie de Lille (FR)
- University of Washington (US)
- Vanderbilt University (US)
- Corresponding author
- Nowicki, Bogdan
- Venue
- Infection and Immunity
- Publisher
- American Society for Microbiology
- Published
- 2004-08
- Volume
- 72
- Issue
- 8
- Pages
- 4827-4835
- ISSN
- 0019-9567 (ISSN-L)
- 1098-5522
- PMC
- PMC470682
- OpenAlex
- W2108619515
- Semantic Scholar
- bc866fb4055df2a2fed42461a9322790f95ba4d4
- Cited by
- 66
- References
- 38
- Citations by year
- 2025: 4 · 2024: 2 · 2023: 3 · 2022: 1 · 2021: 1 · 2020: 1 · 2019: 3 · 2018: 1 · 2017: 1 · 2016: 4 · 2015: 4 · 2014: 4 · 2013: 3 · 2012: 4
- FWCI
- 3.52
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Infection and Immunity (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (5r01dk042029-06)
- U.S. Public Health Service (DG-49862)
- National Institute of Diabetes and Digestive and Kidney Diseases (R37DK018381, DK-42029, DK-18381, R01DK018381)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Endocrinology › Escherichia coli research studies
- Health Sciences › Medicine › Epidemiology › Urinary Tract Infections Management
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Biochemical and Structural Characterization
- Keywords
- Escherichia coli, type IV collagen, CD55, chronic pyelonephritis, urinary tract infection, renal interstitium, bacterial adhesion, isogenic mutants, trans-complementation, mouse model
- MeSH terms
- Animals, Chronic Disease, Disease Models, Animal, Escherichia coli, Escherichia coli Infections, Female, HeLa Cells, Humans, Kidney, Mice, Inbred C3H, Pyelonephritis, Virulence, Adhesins, Bacterial, CD55 Antigens, Collagen Type IV, Escherichia coli Proteins, Mice
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Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
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