The DOI matches its Crossref record; 26 of the 26 references it cites were found in a registry or an index.
The role of a stress‐response protein in Salmonella typhimurium virulence
Abstract
Summary: We recently described the use of selective transposon mutagenesis to generate a series of a virulent mutants of a pathogenic strain of Salmonella typhimurium. Cloning and sequencing of the insertion sites from two of these mutants reveals that both have identical locations within an open reading frame that is highly homologous to a gene, htrA, encoding a heat‐shock protein in Escherichia coli. DNA sequence analysis of S. typhimurium htrA reveals the presence of a gene capable of encoding a protein with a calculated Mr of 49316 that has 88.7% protein:protein homology with its E. coli counterpart. In E. coli, lesions in this gene, also known as degP, reduce proteolytic degradation of aberrant periplasmic proteins. Characteristics of the S. typhimurium htrA mutants, 046 and 014, in vivo and in vitro suggested that they are avirulent because of impaired ability to survive and/or replicate in host tissues. In vitro, the S. typhimurium htrA mutants 046 and 014 are not temperature‐sensitive but were found to be more susceptible to oxidative stress than the parent, suggesting that they may be less able to withstand oxidative killing within macrophages.
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Cites
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Cited by
Role of Heat Shock Proteins in Protection from and Pathogenesis of Infectious Diseases
Zügel, U., & Kaufmann, S. H. E. (1999) · Clinical Microbiology Reviews · doi:10.1128/cmr.12.1.19 · Cited by 559
Pathogenesis of Enteric Infection by Campylobacter
Ketley, J. M. (1997) · Microbiology · doi:10.1099/00221287-143-1-5 · Cited by 411
Pushing the envelope: extracytoplasmic stress responses in bacterial pathogens
Rowley, G., Spector, M., Kormanec, J., & Roberts, M. (2006) · Nature Reviews Microbiology · doi:10.1038/nrmicro1394 · Cited by 321
Pogliano, J., Lynch, A. S., Belin, D., Lin, E. C., & Beckwith, J. (1997) · Genes & Development · doi:10.1101/gad.11.9.1169 · Cited by 306
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Raivio, T. L. (2005) · Molecular Microbiology · doi:10.1111/j.1365-2958.2005.04625.x · Cited by 303
Metadata
- Authors
- Johnson, K., Charles, I., Dougan, G., Pickard, D., O'Gaora, P., Costa, G., Ali, T., Miller, I., & Hormaeche, C.
- Venue
- Molecular Microbiology
- Publisher
- Wiley
- Published
- 1991-02
- Volume
- 5
- Issue
- 2
- Pages
- 401-407
- ISSN
- 0950-382X (ISSN-L)
- 1365-2958
- OpenAlex
- W2110176907
- Cited by
- 273
- References
- 26
- FWCI
- 7.24
- Type
- journal article
- Language
- en
- Open access
- closed
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- Johnson, K., Charles, I., Dougan, G., Pickard, D., O'Gaora, P., Costa, G., Ali, T., Miller, I., & Hormaeche, C. (1991). The role of a stress‐response protein in Salmonella typhimurium virulence. Molecular Microbiology. https://doi.org/10.1111/j.1365-2958.1991.tb02122.x
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
