Cited by 914CRISPR and Genetic EngineeringBacterial Genetics and BiotechnologyBacteriophages and microbial interactions

CRISPR-Cas Systems in Bacteria and Archaea: Versatile Small RNAs for Adaptive Defense and Regulation

Bhaya, Devaki, Davison, Michelle, Barrangou, Rodolphe·2011·Annual Review of Genetics

Abstract

Bacteria and archaea have evolved defense and regulatory mechanisms to cope with various environmental stressors, including virus attack. This arsenal has been expanded by the recent discovery of the versatile CRISPR-Cas system, which has two novel features. First, the host can specifically incorporate short sequences from invading genetic elements (virus or plasmid) into a region of its genome that is distinguished by clustered regularly interspaced short palindromic repeats (CRISPRs). Second, when these sequences are transcribed and precisely processed into small RNAs, they guide a multifunctional protein complex (Cas proteins) to recognize and cleave incoming foreign genetic material. This adaptive immunity system, which uses a library of small noncoding RNAs as a potent weapon against fast-evolving viruses, is also used as a regulatory system by the host. Exciting breakthroughs in understanding the mechanisms of the CRISPR-Cas system and its potential for biotechnological applications and understanding evolutionary dynamics are discussed.

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Crossref · 10.1146/annurev-genet-110410-132430

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Authors
Bhaya, D., Davison, M., & Barrangou, R.
Affiliations
  • Carnegie Institution for Science (US)
  • DANISCO, USA, Inc., Madison, Wisconsin 53716;
Venue
Annual Review of Genetics
Published
2011-12-15
Volume
45
Issue
1
Pages
273-297
ISSN
  • 0066-4197 (ISSN-L)
  • 1545-2948
OpenAlex
W2131527797
Cited by
914
References
122
Citations by year
2026: 19 · 2025: 44 · 2024: 57 · 2023: 51 · 2022: 67 · 2021: 77 · 2020: 46 · 2019: 59 · 2018: 72 · 2017: 75 · 2016: 71 · 2015: 67 · 2014: 78 · 2013: 93 · 2012: 34
FWCI
5.95
Type
journal article
Language
en
Open access
closed
Locations
  • Annual Review of Genetics (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Science Foundation
  • Carnegie Institution for Science
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
  • Physical Sciences › Environmental Science › Ecology › Bacteriophages and microbial interactions
Keywords
CRISPR-Cas, CRISPR, small RNA, adaptive immunity, bacterial defense against viruses, archaea, plasmid defense, non-coding RNA regulation, Cas proteins, genome editing, evolutionary dynamics
MeSH terms
Archaea, Bacteria, Bacterial Proteins, Bacteriophages, Endodeoxyribonucleases, Plasmids, RNA Processing, Post-Transcriptional, RNA, Bacterial, Transcription, Genetic, Virus Diseases, Gene Expression Regulation, Bacterial, Evolution, Molecular, Computational Biology, RNA, Archaeal, Gene Expression Regulation, Archaeal, Escherichia coli Proteins, RNA Interference, Genetic Loci, Adaptive Immunity, RNA, Small Untranslated, CRISPR-Associated Proteins

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