CRISPR-Cas Systems in Bacteria and Archaea: Versatile Small RNAs for Adaptive Defense and Regulation
Abstract
Bacteria and archaea have evolved defense and regulatory mechanisms to cope with various environmental stressors, including virus attack. This arsenal has been expanded by the recent discovery of the versatile CRISPR-Cas system, which has two novel features. First, the host can specifically incorporate short sequences from invading genetic elements (virus or plasmid) into a region of its genome that is distinguished by clustered regularly interspaced short palindromic repeats (CRISPRs). Second, when these sequences are transcribed and precisely processed into small RNAs, they guide a multifunctional protein complex (Cas proteins) to recognize and cleave incoming foreign genetic material. This adaptive immunity system, which uses a library of small noncoding RNAs as a potent weapon against fast-evolving viruses, is also used as a regulatory system by the host. Exciting breakthroughs in understanding the mechanisms of the CRISPR-Cas system and its potential for biotechnological applications and understanding evolutionary dynamics are discussed.
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CRISPRs are related to the adaptive immunity system that has been widely identified and characterized in many archaea and bacteria.
“CRISPRs, a type of important repeat elements, are related to the adaptive immunity system that has been widely identified and characterized in many archaea and bacteria.63 , 64”
From Whole genomic DNA sequencing and comparative genomic analysis of<i>Arthrospira platensis</i>: high genome plasticity and genetic diversity (2016) · cites this paper as [63]
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CRISPR-mediated phage resistance and the ghost of coevolution past
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Analysis of CRISPR system function in plant pathogenXanthomonas oryzae
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Corynebacterium diphtheriae spoligotyping based on combined use of two CRISPR loci
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A Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial Immunity
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Multiplex Genome Engineering Using CRISPR/Cas Systems
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Genome engineering using the CRISPR-Cas9 system
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RNA-Guided Human Genome Engineering via Cas9
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CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes
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RNA-programmed genome editing in human cells
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Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas systems
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Cas9 as a versatile tool for engineering biology
Mali, P., Esvelt, K. M., & Church, G. M. (2013) · Nature Methods · doi:10.1038/nmeth.2649 · Cited by 1,197 · see citing works
CCTop: An intuitive, flexible and reliable CRISPR/Cas9 target prediction tool
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Orthogonal Cas9 proteins for RNA-guided gene regulation and editing
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Classification and evolution of type II CRISPR-Cas systems
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The CRISPR/Cas9 system facilitates clearance of the intrahepatic HBV templates in vivo
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RNA-guided endonuclease provides a therapeutic strategy to cure latent herpesviridae infection
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Molecular biology at the cutting edge: A review on CRISPR/CAS9 gene editing for undergraduates
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“CRISPRs, a type of important repeat elements, are related to the adaptive immunity system that has been widely identified and characterized in many archaea and bacteria.(63,64) We comparatively analysed the distribution of CRISPR and CRISPRassociated protein (Cas) systems among four Arthrospira genomes.”
BackgroundLearning to quantify uncertainty in off-target activity for CRISPR guide RNAs
Özden, F., & Minary, P. (2024) · Nucleic Acids Research · doi:10.1093/nar/gkae759 · Cited by 21 · see citing works
Yaish, O., Asif, M., & Orenstein, Y. (2022) · Briefings in Bioinformatics · doi:10.1093/bib/bbac157 · Cited by 19 · see citing works
CRISPR-Cas mediated genome engineering of cyanobacteria
Sengupta, A., Liu, D., & Pakrasi, H. B. (2022) · Methods in Enzymology · doi:10.1016/bs.mie.2022.07.023 · Cited by 13 · see citing works
- Authors
- Bhaya, D., Davison, M., & Barrangou, R.
- Affiliations
- Carnegie Institution for Science (US)
- DANISCO, USA, Inc., Madison, Wisconsin 53716;
- Venue
- Annual Review of Genetics
- Publisher
- Annual Reviews
- Published
- 2011-12-15
- Volume
- 45
- Issue
- 1
- Pages
- 273-297
- ISSN
- 0066-4197 (ISSN-L)
- 1545-2948
- OpenAlex
- W2131527797
- Cited by
- 914
- References
- 122
- Citations by year
- 2026: 19 · 2025: 44 · 2024: 57 · 2023: 51 · 2022: 67 · 2021: 77 · 2020: 46 · 2019: 59 · 2018: 72 · 2017: 75 · 2016: 71 · 2015: 67 · 2014: 78 · 2013: 93 · 2012: 34
- FWCI
- 5.95
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Annual Review of Genetics (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Science Foundation
- Carnegie Institution for Science
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Physical Sciences › Environmental Science › Ecology › Bacteriophages and microbial interactions
- Keywords
- CRISPR-Cas, CRISPR, small RNA, adaptive immunity, bacterial defense against viruses, archaea, plasmid defense, non-coding RNA regulation, Cas proteins, genome editing, evolutionary dynamics
- MeSH terms
- Archaea, Bacteria, Bacterial Proteins, Bacteriophages, Endodeoxyribonucleases, Plasmids, RNA Processing, Post-Transcriptional, RNA, Bacterial, Transcription, Genetic, Virus Diseases, Gene Expression Regulation, Bacterial, Evolution, Molecular, Computational Biology, RNA, Archaeal, Gene Expression Regulation, Archaeal, Escherichia coli Proteins, RNA Interference, Genetic Loci, Adaptive Immunity, RNA, Small Untranslated, CRISPR-Associated Proteins
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