The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
The PrsA lipoprotein is essential for protein secretion in Bacillus subtilis and sets a limit for high‐level secretion
Abstract
Summary: Mutations of the prsA gene of Bacillus subtilis have indicated that the gene is involved in protein secretion and it encodes a novel component of the cellular secretion machinery. We now demonstrate that the gene product is a membrane‐associated lipoprotein, presumably bound to the outer face of the cytoplasmic membrane. Experiments to inactivate the prsA gene with insertions indicated that it is indispensable for viability. The cellular level of PrsA protein was shown to be decreased in prsA mutants with decreased level of exoproteins, consistent with an essential function in protein secretion. An increased amount of cellular PrsA protein was introduced by Increasing the copy number of prsA in B. subtilis. This enhanced, from six‐ to twofold, the secretion of α‐amylases and a protease in strains, which expressed high levels of these exoenzymes. This suggests that PrsA protein is the rate‐limiting component of the secretion machinery, a finding that is of considerable biotechnological interest.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Hartl, F.-U., Lecker, S., Schiebel, E., Hendrick, J. P., & Wickner, W. (1990) · Cell · doi:10.1016/0092-8674(90)90160-g · Cited by 562
Brundage, L., Hendrick, J. P., Schiebel, E., Driessen, A. J. M., & Wickner, W. (1990) · Cell · doi:10.1016/0092-8674(90)90111-q · Cited by 505
Cited by
Allelic replacement in Staphylococcus aureus with inducible counter-selection
Bae, T., & Schneewind, O. (2006) · Plasmid · doi:10.1016/j.plasmid.2005.05.005 · Cited by 621
Proteomics of Protein Secretion by Bacillus subtilis : Separating the “Secrets” of the Secretome
Tjalsma, H., Antelmann, H., Jongbloed, J. D. H., Braun, P. G., Darmon, E., Dorenbos, R., Dubois, J.-Y. F., Westers, H., Zanen, G., Quax, W. J., et al. (2004) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.68.2.207-233.2004 · Cited by 579
Westers, L., Westers, H., & Quax, W. J. (2004) · Biochimica et Biophysica Acta (BBA) - Molecular Cell Research · doi:10.1016/j.bbamcr.2004.02.011 · Cited by 490
Functional microdomains in bacterial membranes
López, D., & Kolter, R. (2010) · Genes & Development · doi:10.1101/gad.1945010 · Cited by 346
Gasser, B., Saloheimo, M., Rinas, U., Dragosits, M., Rodríguez-Carmona, E., Baumann, K., Giuliani, M., Parrilli, E., Branduardi, P., Lang, C., et al. (2008) · Microbial Cell Factories · doi:10.1186/1475-2859-7-11 · Cited by 326
Metadata
- Authors
- Kontinen, V. P., & Sarvas, M.
- Venue
- Molecular Microbiology
- Publisher
- Wiley
- Published
- 1993-05
- Volume
- 8
- Issue
- 4
- Pages
- 727-737
- ISSN
- 0950-382X (ISSN-L)
- 1365-2958
- OpenAlex
- W1993802761
- Cited by
- 209
- References
- 2
- FWCI
- 1.82
- Type
- journal article
- Language
- en
- Open access
- closed
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: The PrsA lipoprotein is essential for protein secretion in Bacillus subtilis and sets a limit for high‐level secretion. https://app.cochat.ai/papers/10.1111/j.1365-2958.1993.tb01616.x
- The paper
- Kontinen, V. P., & Sarvas, M. (1993). The PrsA lipoprotein is essential for protein secretion in Bacillus subtilis and sets a limit for high‐level secretion. Molecular Microbiology. https://doi.org/10.1111/j.1365-2958.1993.tb01616.x
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
