The DOI matches its Crossref record; 144 of the 144 references it cites were found in a registry or an index.
Proteomics of Protein Secretion by Bacillus subtilis : Separating the “Secrets” of the Secretome
Open accessAbstract
SUMMARY: Secretory proteins perform a variety of important“ remote-control” functions for bacterial survival in the environment. The availability of complete genome sequences has allowed us to make predictions about the composition of bacterial machinery for protein secretion as well as the extracellular complement of bacterial proteomes. Recently, the power of proteomics was successfully employed to evaluate genome-based models of these so-called secretomes. Progress in this field is well illustrated by the proteomic analysis of protein secretion by the gram-positive bacterium Bacillus subtilis , for which ∼90 extracellular proteins were identified. Analysis of these proteins disclosed various“ secrets of the secretome,” such as the residence of cytoplasmic and predicted cell envelope proteins in the extracellular proteome. This showed that genome-based predictions reflect only∼ 50% of the actual composition of the extracellular proteome of B. subtilis . Importantly, proteomics allowed the first verification of the impact of individual secretion machinery components on the total flow of proteins from the cytoplasm to the extracellular environment. In conclusion, proteomics has yielded a variety of novel leads for the analysis of protein traffic in B. subtilis and other gram-positive bacteria. Ultimately, such leads will serve to increase our understanding of virulence factor biogenesis in gram-positive pathogens, which is likely to be of high medical relevance.
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The complete genome sequence of the gram-positive bacterium Bacillus subtilis.
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Cited by
Chen, X. H., Koumoutsi, A., Scholz, R., Eisenreich, A., Schneider, K., Heinemeyer, I., Morgenstern, B., Voss, B., Hess, W. R., Reva, O., et al. (2007) · Nature Biotechnology · doi:10.1038/nbt1325 · Cited by 873
Biosynthesis and Mode of Action of Lantibiotics
Chatterjee, C., Paul, M., Xie, L., & van der Donk, W. A. (2005) · Chemical Reviews · doi:10.1021/cr030105v · Cited by 718
A comprehensive review of signal peptides: Structure, roles, and applications
Owji, H., Nezafat, N., Negahdaripour, M., Hajiebrahimi, A., & Ghasemi, Y. (2018) · European Journal of Cell Biology · doi:10.1016/j.ejcb.2018.06.003 · Cited by 523
Plant secretome: Unlocking secrets of the secreted proteins
Agrawal, G. K., Jwa, N., Lebrun, M., Job, D., & Rakwal, R. (2010) · PROTEOMICS · doi:10.1002/pmic.200900514 · Cited by 311
Masquerading microbial pathogens: capsular polysaccharides mimic host-tissue molecules
Cress, B. F., Englaender, J. A., He, W., Kasper, D., Linhardt, R. J., & Koffas, M. A. G. (2014) · FEMS Microbiology Reviews · doi:10.1111/1574-6976.12056 · Cited by 282
Metadata
- Authors
- Tjalsma, H., Antelmann, H., Jongbloed, J. D. H., Braun, P. G., Darmon, E., Dorenbos, R., Dubois, J.-Y. F., Westers, H., Zanen, G., Quax, W. J., Kuipers, O. P., Bron, S., Hecker, M., & van Dijl, J. M.
- Venue
- Microbiology and Molecular Biology Reviews
- Publisher
- American Society for Microbiology
- Published
- 2004-06
- Volume
- 68
- Issue
- 2
- Pages
- 207-233
- ISSN
- 1092-2172 (ISSN-L)
- 1098-5557
- OpenAlex
- W2116788456
- Cited by
- 579
- References
- 144
- FWCI
- 2.07
- Type
- journal article
- Language
- en
- Open access
- green
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- Tjalsma, H., Antelmann, H., Jongbloed, J. D. H., Braun, P. G., Darmon, E., Dorenbos, R., Dubois, J.-Y. F., Westers, H., Zanen, G., Quax, W. J., Kuipers, O. P., Bron, S., Hecker, M., & van Dijl, J. M. (2004). Proteomics of Protein Secretion by Bacillus subtilis : Separating the “Secrets” of the Secretome. Microbiology and Molecular Biology Reviews. https://doi.org/10.1128/mmbr.68.2.207-233.2004
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