Asymmetric cross‐regulation between the nitrate‐responsive NarX–NarL and NarQ–NarP two‐component regulatory systems from Escherichia coli K‐12

Noriega, Chris E.; Lin, Hsia‐Yin; Chen, Li‐Ling; Williams, Stanly B.; Stewart, Valley

doi:10.1111/j.1365-2958.2009.06987.x

Cited by 108Bacterial Genetics and BiotechnologyGene Regulatory Network AnalysisEscherichia coli research studies

Asymmetric cross‐regulation between the nitrate‐responsive NarX–NarL and NarQ–NarP two‐component regulatory systems from Escherichia coli K‐12

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Noriega, Chris E., Lin, Hsia‐Yin, Chen, Li‐Ling, Williams, Stanly B., Stewart, Valley·2010·Molecular Microbiology

DOI 10.1111/j.1365-2958.2009.06987.xPMC PMC3034140

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Abstract

The NarX-NarL and NarQ-NarP sensor-response regulator pairs control Escherichia coli gene expression in response to nitrate and nitrite. Previous analysis suggests that the Nar two-component systems form a cross-regulation network in vivo. Here we report on the kinetics of phosphoryl transfer between different sensor-regulator combinations in vitro. NarX exhibited a noticeable kinetic preference for NarL over NarP, whereas NarQ exhibited a relatively slight kinetic preference for NarL. These findings were substantiated in reactions containing one sensor and both response regulators, or with two sensors and a single response regulator. We isolated 21 NarX mutants with missense substitutions in the cytoplasmic central and transmitter modules. These confer phenotypes that reflect defects in phospho-NarL dephosphorylation. Five of these mutants, all with substitutions in the transmitter DHp domain, also exhibited NarP-blind phenotypes. Phosphoryl transfer assays in vitro confirmed that these NarX mutants have defects in catalysing NarP phosphorylation. By contrast, the corresponding NarQ mutants conferred phenotypes indicating comparable interactions with both NarP and NarL. Our overall results reveal asymmetry in the Nar cross-regulation network, such that NarQ interacts similarly with both response regulators, whereas NarX interacts preferentially with NarL.

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NarGHI is highly oxygen-responsive, under primary transcriptional control via the oxygen sensor FNR, and appears to be the major contributor to respiratory anaerobic activity.
“The first, NarGHI, is highly oxygen-responsive, under primary transcriptional control via the oxygen sensor fumarate nitrate reductase regulator (FNR), and appears to be the major contributor to respiratory anaerobic activity[256–258].”
From Enterosalivary nitrate metabolism and the microbiome: Intersection of microbial metabolism, nitric oxide and diet in cardiac and pulmonary vascular health (2017) · cites this paper as [258]

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Authors: Noriega, C. E., Lin, H., Chen, L., Williams, S. B., & Stewart, V.
Affiliations: University of California, Davis (US)
University of Utah (US)
Corresponding author: Stewart, Valley
ORCID: Chen, Li‐Ling
Stewart, Valley
Venue: Molecular Microbiology
Publisher: Wiley
Published: 2010-01
Volume: 75
Issue: 2
Pages: 394-412
ISSN: 0950-382X (ISSN-L)
1365-2958
DOI: 10.1111/j.1365-2958.2009.06987.x
PMC: PMC3034140
OpenAlex: W2171374540
Cited by: 108
References: 72
Citations by year: 2026: 4 · 2025: 9 · 2024: 7 · 2023: 13 · 2022: 10 · 2021: 14 · 2020: 5 · 2019: 6 · 2018: 2 · 2017: 2 · 2016: 7 · 2015: 4 · 2014: 4 · 2013: 4 · 2012: 10
FWCI: 3.29
Type: journal article
Language: en
Open access: green
www.ncbi.nlm.nih.gov
Licence: onlinelibrary.wiley.com/termsAndConditions#vor (vor)
Locations: Molecular Microbiology (publishedVersion)
PubMed (publishedVersion)
PubMed Central (acceptedVersion)
Funders: U.S. Public Health Service
National Institute of General Medical Sciences (GM036877, R01GM036877)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gene Regulatory Network Analysis
Life Sciences › Biochemistry, Genetics and Molecular Biology › Endocrinology › Escherichia coli research studies
Keywords: two-component systems, cross-regulation, dephosphorylation, missense mutants, nitrate signaling, Escherichia coli K-12
MeSH terms: DNA-Binding Proteins, Homeostasis, Kinetics, Membrane Proteins, Phenotype, Phosphoproteins, Phosphorylation, Phosphotransferases, Protein Kinases, Gene Expression Regulation, Bacterial, Amino Acid Substitution, Escherichia coli Proteins, Escherichia coli K12

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Asymmetric cross‐regulation between the nitrate‐responsive NarX–NarL and NarQ–NarP two‐component regulatory systems from Escherichia coli K‐12

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