How does the oxidative burst of macrophages kill bacteria? Still an open question
Open accessAbstract
Summary: Reactive oxygen species (ROS) are critical components of the antimicrobial repertoire of macrophages, yet the mechanisms by which ROS damage bacteria in the phagosome are unclear. The NADH‐dependent phagocytic oxidase produces superoxide, which dismutes to form H 2 O 2 . The Barras and Méresse labs use a GFP fusion to an OxyR regulated gene to show that phagocyte‐derived H 2 O 2 is gaining access to the Salmonella cytoplasm. However, they have also shown previously that Salmonella has redundant systems to detoxify this H 2 O 2 . Although Salmonella propagate in a unique vacuole, their data suggest that ROS are not diminished in this modified phagosome. These recent results are put into the context of our overall understanding of potential oxidative bacterial damage occurring in macrophages.
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M1-like macrophages contain the lethal antimicrobial •NO and can kill neutrophil-resistant organisms.
“They contain the lethal antimicrobial •NO and can kill neutrophil-resistant organisms.”
From The Role of Reactive Species on Innate Immunity (2022) · cites this paper as [49]
Neutrophils are particularly effective at killing invading bacteria by engulfing them, activating the respiratory burst, and generating lethal oxidative molecules such as hydrogen peroxide and hypochlorite ions, which kill most invading bacteria.
“neutrophils, which are particularly effective at killing invading bacteria by engulfing them, activating the respiratory burst, and generating lethal oxidative molecules such as hydrogen peroxide and hypochlorite ions, which kill most invading bacteria”
From The Role of Reactive Species on Innate Immunity (2022) · cites this paper as [49]
Recognition of bacteria by macrophages leads to ROS production in different cellular compartments, where they fulfill different antibacterial functions.
“Recognition of bacteria by macrophages leads to ROS production in different cellular compartments, where they fulfill different antibacterial functions.”
From Functions of ROS in Macrophages and Antimicrobial Immunity (2021) · cites this paper as [235]
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- Authors
- Slauch, J. M.
- Affiliations
- University of Illinois Urbana-Champaign (US)
- Corresponding author
- Slauch, James M.
- ORCID
- Slauch, James M.
- Venue
- Molecular Microbiology
- Publisher
- Wiley
- Published
- 2011-03-14
- Volume
- 80
- Issue
- 3
- Pages
- 580-583
- ISSN
- 0950-382X (ISSN-L)
- 1365-2958
- PMC
- PMC3109634
- OpenAlex
- W1652069479
- Cited by
- 436
- References
- 18
- Citations by year
- 2026: 16 · 2025: 18 · 2024: 33 · 2023: 28 · 2022: 51 · 2021: 41 · 2020: 40 · 2019: 41 · 2018: 40 · 2017: 26 · 2016: 30 · 2015: 24 · 2014: 20 · 2013: 11 · 2012: 13
- FWCI
- 6.23
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Molecular Microbiology (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Institutes of Health
- National Institute of Allergy and Infectious Diseases (R01AI063230, R01AI080705, AI080705)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
- Life Sciences › Immunology and Microbiology › Immunology › Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Keywords
- reactive oxygen species, Salmonella, phagosome, superoxide, hydrogen peroxide, NADPH oxidase, OxyR, bacterial detoxification, bacterial cytoplasm, GFP fusion protein
- MeSH terms
- Hydrogen Peroxide, Macrophages, Inactivation, Metabolic, Models, Biological, Phagosomes, Salmonella, Superoxides, Respiratory Burst, Reactive Oxygen Species, Microbial Viability
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