The DOI matches its Crossref record; 54 of the 54 references it cites were found in a registry or an index.
MicroRNA166 controls root and nodule development in Medicago truncatula
Open accessAbstract
Summary: Legume root architecture is characterized by the development of two de novo meristems, leading to the formation of lateral roots or symbiotic nitrogen‐fixing nodules. Organogenesis involves networks of transcription factors, the encoding mRNAs of which are frequently targets of microRNA (miRNA) regulation. Most plant miRNAs, in contrast with animal miRNAs, are encoded as single entities in an miRNA precursor. In the model legume Medicago truncatula, we have identified the MtMIR166a precursor containing tandem copies of MIR166 in a single transcriptional unit. These miRNAs post‐transcriptionally regulate a new family of transcription factors associated with nodule development, the class‐III homeodomain‐leucine zipper (HD‐ZIP III) genes. In situ expression analysis revealed that these target genes are spatially co‐expressed with MIR166 in vascular bundles, and in apical regions of roots and nodules. Overexpression of the tandem miRNA precursor correlated with MIR166 accumulation and the downregulation of several class‐III HD‐ZIP genes, indicating its functionality. MIR166 overexpression reduced the number of symbiotic nodules and lateral roots, and induced ectopic development of vascular bundles in these transgenic roots. Hence, plant polycistronic miRNA precursors, although rare, can be processed, and MIR166‐mediated post‐transcriptional regulation is a new regulatory pathway involved in the regulation of legume root architecture.
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Cites
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Cited by
Roles of Plant Small RNAs in Biotic Stress Responses
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miR398 and miR408 are up-regulated in response to water deficit in Medicago truncatula
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Plet, J., Wasson, A., Ariel, F., Le Signor, C., Baker, D., Mathesius, U., Crespi, M., & Frugier, F. (2011) · The Plant Journal · doi:10.1111/j.1365-313x.2010.04447.x · Cited by 304
Self-citation
Metadata
- Authors
- Boualem, A., Laporte, P., Jovanovic, M., Laffont, C., Plet, J., Combier, J., Niebel, A., Crespi, M., & Frugier, F.
- Venue
- The Plant Journal
- Publisher
- Wiley
- Published
- 2008-02-21
- Volume
- 54
- Issue
- 5
- Pages
- 876-887
- ISSN
- 0960-7412 (ISSN-L)
- 1365-313X
- OpenAlex
- W2004016211
- Cited by
- 311
- References
- 54
- FWCI
- 22.75
- Type
- journal article
- Language
- en
- Open access
- green
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- Boualem, A., Laporte, P., Jovanovic, M., Laffont, C., Plet, J., Combier, J., Niebel, A., Crespi, M., & Frugier, F. (2008). MicroRNA166 controls root and nodule development in Medicago truncatula. The Plant Journal. https://doi.org/10.1111/j.1365-313x.2008.03448.x
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