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Cited by 241Epigenetics and DNA MethylationPlant Stress Responses and ToleranceHeavy metals in environment

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Specific hypomethylation of DNA is induced by heavy metals in white clover and industrial hemp

Aina, Roberta, Sgorbati, Sergio, Santagostino, Angela, Labra, Massimo, Ghiani, Alessandra, Citterio, Sandra·2004·Physiologia Plantarum
DOI 10.1111/j.1399-3054.2004.00343.x
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Abstract

The present study was to assess the effect of heavy metal stress on the DNA methylation of a metal‐sensitive plant, Trifolium repens L. and of a metal‐tolerant plant, Cannabis sativa L. The changes in the level of 5‐methylcytosine (5mC) in the root DNA of plants grown on soils contaminated with different concentrations of Ni 2+, Cd 2+ and Cr 6+ compared with that of untreated plants, were determined by immunolabelling with a monoclonal antibody, using the Slot‐Blot technique. Results showed that DNA of hemp control plants was about three times more methylated than clover DNA, for the same amount of root DNA. Heavy metal treatments induced a global dose‐dependent decrease of 5mC content, both in hemp and clover, ranging from 20 to 40%. Changes in methylation pattern of 5′‐CCGG‐3′ containing sequences were investigated by methylation‐sensitive amplification polymorphism (MSAP) technique. Control plants of the same species showed a very similar pattern, suggesting that, in normal condition, methylation involves precise sites. Heavy metals induced DNA methylation changes mainly related to hypomethylation events. These variations were not randomly directed but involved specific DNA sequences, since the detected polymorphisms were the same in all the plants analysed for each treatment.

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Cited by

Metadata

Authors
Aina, R., Sgorbati, S., Santagostino, A., Labra, M., Ghiani, A., & Citterio, S.
Affiliations
  • University of Milano-Bicocca (IT)
  • University of Milan (IT)
Corresponding author
Aina, Roberta
ORCID
Venue
Physiologia Plantarum
Publisher
Wiley
Published
2004-06-23
Volume
121
Issue
3
Pages
472-480
ISSN
  • 0031-9317 (ISSN-L)
  • 1399-3054
DOI
10.1111/j.1399-3054.2004.00343.x
OpenAlex
W2138766691
Cited by
241
References
3
Citations by year
2026: 6 · 2025: 8 · 2024: 15 · 2023: 15 · 2022: 11 · 2021: 21 · 2020: 14 · 2019: 9 · 2018: 16 · 2017: 15 · 2016: 9 · 2015: 13 · 2014: 16 · 2013: 19 · 2012: 11
FWCI
0.83
Type
journal article
Language
en
Open access
closed
Licence
onlinelibrary.wiley.com/termsAndConditions#vor (vor)
Locations
  • Physiologia Plantarum (publishedVersion)
  • BOA (University of Milano-Bicocca) (submittedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Epigenetics and DNA Methylation
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Stress Responses and Tolerance
  • Physical Sciences › Environmental Science › Pollution › Heavy metals in environment
Keywords
DNA methylation, DNA hypomethylation, heavy metal stress, nickel, cadmium, hexavalent chromium, white clover, Cannabis sativa, industrial hemp, 5-methylcytosine, metal-tolerant plants, soil contamination, methylation-sensitive amplification polymorphism

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