Student or educator? Sign up with your school email and get 6 months of Pro free.
CoChat
Sign Up
Cited by 241Epigenetics and DNA MethylationPlant Stress Responses and ToleranceHeavy metals in environment

The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.

Specific hypomethylation of DNA is induced by heavy metals in white clover and industrial hemp

Aina, Roberta, Sgorbati, Sergio, Santagostino, Angela, Labra, Massimo, Ghiani, Alessandra, Citterio, Sandra·2004·Physiologia Plantarum
DOI 10.1111/j.1399-3054.2004.00343.x
Open this paper in CoChat — free account

Abstract

The present study was to assess the effect of heavy metal stress on the DNA methylation of a metal‐sensitive plant, Trifolium repens L. and of a metal‐tolerant plant, Cannabis sativa L. The changes in the level of 5‐methylcytosine (5mC) in the root DNA of plants grown on soils contaminated with different concentrations of Ni 2+, Cd 2+ and Cr 6+ compared with that of untreated plants, were determined by immunolabelling with a monoclonal antibody, using the Slot‐Blot technique. Results showed that DNA of hemp control plants was about three times more methylated than clover DNA, for the same amount of root DNA. Heavy metal treatments induced a global dose‐dependent decrease of 5mC content, both in hemp and clover, ranging from 20 to 40%. Changes in methylation pattern of 5′‐CCGG‐3′ containing sequences were investigated by methylation‐sensitive amplification polymorphism (MSAP) technique. Control plants of the same species showed a very similar pattern, suggesting that, in normal condition, methylation involves precise sites. Heavy metals induced DNA methylation changes mainly related to hypomethylation events. These variations were not randomly directed but involved specific DNA sequences, since the detected polymorphisms were the same in all the plants analysed for each treatment.

Overview

Integrity signals

Registry

Crossref · 10.1111/j.1399-3054.2004.00343.x

Cites

3 of 3 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Verification

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Citations

Cites

Research works printed but not linked: 66

  1. 10.1111/j.1399-3054.1997.tb04778.x · DOI
  2. 10.1002/j.1460-2075.1988.tb03072.x · DOI
  3. 10.1016/S0968-0004(98)01225-0 · DOI
  4. 10.1016/0014-5793(94)00850-7 · DOI
  5. 10.1016/S0378-4274(01)00484-2 · DOI
  6. 10.1046/j.1469-8137.2002.00506.x · DOI · Paper page
  7. Briat JF, 1999, Plant responses to metal toxicity. Comptes Rendus de l'Academie des Sciences, Serie III, Sci la Vie/Life Sci, 322, 43
  8. 10.1016/S0176-1617(99)80295-8 · DOI
  9. 10.1080/15226510208500080 · DOI
  10. 10.1016/S1383-5742(96)00050-6 · DOI
  11. 10.1007/s00438-002-0772-4 · DOI · Paper page
  12. Cheah MS, 1984, Hypomethylation of DNA in human cancer cells: a site‐specific change in the c‐myc oncogene, J Natl Cancer Inst, 73, 1057
  13. 10.1021/es0157550 · DOI
  14. 10.1016/0378-4274(95)03512-5 · DOI
  15. 10.1016/S0145-2126(98)00167-2 · DOI
  16. Cumming JR, 1990, Stress Responses in Plants: Adaptation and Acclimation Mechanisms, 329
  17. 10.1046/j.1365-3040.2002.00843.x · DOI
  18. 10.1023/A:1006427226972 · DOI
  19. 10.1146/annurev.arplant.49.1.223 · DOI · Paper page
  20. 10.1016/0027-5107(88)90096-6 · DOI
  21. 10.1007/BF00162972 · DOI
  22. 10.1016/S1383-5742(96)00047-6 · DOI
  23. 10.1038/292860a0 · DOI · Paper page
  24. 10.1016/S0959-437X(00)00182-9 · DOI
  25. Hagar H, 1996, Role of reactive oxygen metabolites in DNA damage and cell death in chemical hypoxic injury to LLC‐PK1 cells, Am J Physiol, 271, 209
  26. 10.1016/S0027-5107(01)00078-1 · DOI
  27. 10.1023/A:1005954525087 · DOI · Paper page
  28. 10.1016/0958-1669(95)80024-7 · DOI
  29. Kovacs M, 1986, Bioindication: a short review on the use of plants as indicators of heavy metals, Acta Biologica Hungarica, 37, 19
  30. 10.1016/S0027-5107(03)00103-9 · DOI
  31. 10.1007/s001220050563 · DOI
  32. 10.1055/s-2002-37398 · DOI
  33. 10.1007/BF00282641 · DOI
  34. 10.1016/S1383-5718(98)00078-3 · DOI
  35. Lindsay WL, 1969, Development of a DTPA micronutrient soil test, Agron Abstract, 69, 87
  36. Liu X, 2003, Differential expression of genes regulated in response to drought or salinity stress in sunflower, Crop Sci, 43, 678, 10.2135/cropsci2003.6780 · DOI
  37. 10.1023/A:1023926225931 · DOI
  38. 10.1046/j.1365-294x.2000.01011.x · DOI
  39. 10.1007/BF00015069 · DOI
  40. 10.1046/j.1365-313X.1997.12020313.x · DOI
  41. 10.1034/j.1399-3054.2001.1130401.x · DOI
  42. 10.1006/geno.1993.1097 · DOI
  43. 10.1007/s002040050562 · DOI
  44. 10.1016/0167-4781(90)90100-G · DOI
  45. 10.1093/nar/26.4.1056 · DOI
  46. 10.1016/S1369-5266(00)00147-3 · DOI
  47. 10.1016/0020-711X(93)90249-E · DOI
  48. 10.1080/15226510208500073 · DOI
  49. 10.1104/pp.109.4.1141 · DOI
  50. 10.1007/s004380050374 · DOI · Paper page
  51. 10.1023/A:1004328816645 · DOI · Paper page
  52. Robinson NJ, 1993, Stress‐Induced Gene Expression in Plants, 209
  53. 10.1065/espr2002.10.138 · DOI
  54. 10.1016/S0168-9452(01)00517-9 · DOI
  55. 10.1016/S0958-1669(98)80118-3 · DOI
  56. 10.1006/jmbi.2000.4046 · DOI
  57. Sörensen MB, 1996, Hordein promoter methylation and transcriptional activity in wild‐type and mutant barley endosperm, Mol Gen Genet, 250, 750, 10.1007/BF02172987 · DOI
  58. Steffens JC, 1990, Stress Responses in Plants: Adaptation and Acclimation Mechanisms, 377
  59. 10.1016/S1383-5742(00)00005-3 · DOI
  60. 10.1016/S0027-5107(97)00003-1 · DOI
  61. 10.1016/0092-8674(83)90514-7 · DOI
  62. Winston GW, 1990, Stress Responses in Plants: Adaptation and Acclimation Mechanisms, 57
  63. 10.1007/s004380050986 · DOI
  64. 10.1002/1522-2683(200106)22:10<1946::AID-ELPS1946>3.0.CO;2-Y · DOI
  65. 10.1080/03601239509372954 · DOI
  66. 10.1016/S0378-1119(96)00422-2 · DOI · Paper page

Cited by

See all 241

Metadata

Authors
Aina, R., Sgorbati, S., Santagostino, A., Labra, M., Ghiani, A., & Citterio, S.
Affiliations
  • University of Milano-Bicocca (IT)
  • University of Milan (IT)
Corresponding author
Aina, Roberta
ORCID
Venue
Physiologia Plantarum
Publisher
Wiley
Published
2004-06-23
Volume
121
Issue
3
Pages
472-480
ISSN
  • 0031-9317 (ISSN-L)
  • 1399-3054
DOI
10.1111/j.1399-3054.2004.00343.x
OpenAlex
W2138766691
Cited by
241
References
3
Citations by year
2026: 6 · 2025: 8 · 2024: 15 · 2023: 15 · 2022: 11 · 2021: 21 · 2020: 14 · 2019: 9 · 2018: 16 · 2017: 15 · 2016: 9 · 2015: 13 · 2014: 16 · 2013: 19 · 2012: 11
FWCI
0.83
Type
journal article
Language
en
Open access
closed
Licence
onlinelibrary.wiley.com/termsAndConditions#vor (vor)
Locations
  • Physiologia Plantarum (publishedVersion)
  • BOA (University of Milano-Bicocca) (submittedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Epigenetics and DNA Methylation
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Stress Responses and Tolerance
  • Physical Sciences › Environmental Science › Pollution › Heavy metals in environment
Keywords
DNA methylation, DNA hypomethylation, heavy metal stress, nickel, cadmium, hexavalent chromium, white clover, Cannabis sativa, industrial hemp, 5-methylcytosine, metal-tolerant plants, soil contamination, methylation-sensitive amplification polymorphism

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

Data sources

Bibliographic data: Semantic Scholar, OpenAlex, Crossref.

Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.