Life stage specific expression of a myosin heavy chain in the hydrozoan Podocoryne carnea
Abstract
In order to study life stage specific gene-expression and also to investigate the molecular components of jellyfish muscle we cloned a tissue specifically expressed cDNA of the metagenetic hydrozoan Podocoryne carnea. A monoclonal antibody was made that reacts with the filaments of striated muscle of the medusa stage. The mAb detects a protein of 220 kDa in Western blots. The antibody was used to clone a cDNA from an expression library. Sequence analysis showed that the clone codes for the rod part of a myosing heavy chain (MHC). Surprisingly, the sequence is more similar to MHCs from striated muscle than to smooth muscle or non-muscle MHCs from either invertebrates or vertebrates. A particularly well conserved region, which may be correlated to a function in striated muscle, was found. Expression studies showed that the corresponding RNA is only present in the striated muscle cells of the medusa. During medusa development, the transcript became detectable at the same time as muscle differentiation becomes visible in the electron microscops.
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Cnidarians and bilaterians share numerous genes for transcription factors, signaling components, and cell-type proteins.
“The cnidarians share with bilaterians many genes encoding transcription factors, signaling pathway components, and cell type-specific proteins (e.g., Yanze et al., 2001;Schuchert et al., 1993;Gro ¨ger et al., 1999;Mu ¨ller et al., 1999;Smith et al., 1999;Spring et al., 2000).”
From Precambrian Animal Life: Probable Developmental and Adult Cnidarian Forms from Southwest China (2002) · cites this paper as Schuchert et al., 1993
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- Authors
- Schuchert, P., Reber-Müller, S., & Schmid, V.
- Affiliations
- University of Basel (CH)
- Corresponding author
- Schuchert, Peter
- Venue
- Differentiation
- Publisher
- Elsevier BV
- Published
- 1993-08
- Volume
- 54
- Issue
- 1
- Pages
- 11-18
- ISSN
- 0301-4681 (ISSN-L)
- OpenAlex
- W2023165163
- Cited by
- 41
- References
- 0
- Citations by year
- 2026: 1 · 2025: 1 · 2020: 1 · 2019: 1 · 2018: 1 · 2017: 2 · 2015: 3 · 2012: 2
- FWCI
- 2.01
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Differentiation (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung (3-30717.91)
- Topics
- Physical Sciences › Earth and Planetary Sciences › Paleontology › Marine Invertebrate Physiology and Ecology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
- Keywords
- myosin heavy chain, Podocoryne carnea, hydrozoan jellyfish, Medusa, striated muscle, monoclonal antibodies, cDNA cloning, myogenesis, conserved domains
- MeSH terms
- Amino Acid Sequence, Animals, Cloning, Molecular, Cnidaria, Molecular Sequence Data, Myosins, Phylogeny, Gene Expression, Sequence Alignment
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