The DOI matches its Crossref record; 15 of the 15 references it cites were found in a registry or an index.
The Determination of Molecular Weight of Bacterial Genome DNA from Renaturation Rates
Abstract
A new method is described for the determination of the total molecular weight of haploid genome DNA. It is based on initial optical renaturation rate measurements of precisely known concentrations of fragmented DNA. The theoretical basis of the measurement is presented. The actual state of replication of the genome has little effect. The exact conditions and the effects of DNA concentration, renaturation time, size of DNA fragments, buffer concentration, optimal temperature and % (G + C) have been determined. Several types of bacterial DNA of known genome size were included as a control. As an application, the DNA genome size have been determined of 40 different bacteria. The method appears to offer advantages by its simplicity, rapidity and reproducibility.
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Cites
A procedure for the isolation of deoxyribonucleic acid from micro-organisms
Marmur, J. (1961) · Journal of Molecular Biology · doi:10.1016/s0022-2836(61)80047-8 · Cited by 11,545
Marmur, J., & Doty, P. (1962) · Journal of Molecular Biology · doi:10.1016/s0022-2836(62)80066-7 · Cited by 4,091
The Quantitative Measurement of DNA Hybridization from Renaturation Rates
Ley, J. D., Cattoir, H., & Reynaerts, A. (1970) · European Journal of Biochemistry · doi:10.1111/j.1432-1033.1970.tb00830.x · Cited by 2,595
Self-citationSchaechter, M., MaalOe, O., & Kjeldgaard, N. O. (1958) · Journal of General Microbiology · doi:10.1099/00221287-19-3-592 · Cited by 1,435
Kinetics of renaturation of DNA
Wetmur, J. G., & Davidson, N. (1968) · Journal of Molecular Biology · doi:10.1016/0022-2836(68)90414-2 · Cited by 1,422
Chromosome replication and the division cycle of Escherichia coli
Cooper, S., & Helmstetter, C. E. (1968) · Journal of Molecular Biology · doi:10.1016/0022-2836(68)90425-7 · Cited by 1,197
The Chromosome of Escherichia coli
Cairns, J. (1963) · Cold Spring Harbor Symposia on Quantitative Biology · doi:10.1101/sqb.1963.028.01.011 · Cited by 409
Replication and recombination of genetic material
Peacock, W. J., & Brock, R. D. (1968) · Australian Academy of Science eBooks · Cited by 324
Denaturation and Renaturation of Deoxyribonucleic Acid12
Mahmuh, J., Rownd, R. H., & Schildkbatjt, C. L. (1963) · Progress in nucleic acid research and molecular biology · doi:10.1016/s0079-6603(08)60644-3 · Cited by 156
Compositional nucleotide distribution and the theoretical prediction of homology in bacterial DNA
Ley, J. D. (1969) · Journal of Theoretical Biology · doi:10.1016/0022-5193(69)90082-4 · Cited by 71
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Cited by
Studies on the spectrophotometric determination of DNA hybridization from renaturation rates
Huss, V. A. R., Festl, H., & Schleifer, K. H. (1983) · Systematic and Applied Microbiology · doi:10.1016/s0723-2020(83)80048-4 · Cited by 1,648
Swings, J., & De Ley, J. (1977) · Bacteriological Reviews · doi:10.1128/br.41.1.1-46.1977 · Cited by 655
Total bacterial diversity in soil and sediment communities—A review
Torsvik, V, Sørheim, R., & Goksøyr, J. (1996) · Journal of Industrial Microbiology & Biotechnology · doi:10.1007/bf01574690 · Cited by 517
Restriction endonucleases for pulsed field mapping of bacterial genomes
McClelland, M., Jones, R., Patel, Y., & Nelson, M. (1987) · Nucleic Acids Research · doi:10.1093/nar/15.15.5985 · Cited by 295
Metadata
- Authors
- Gillis, M., Ley, J. D., & Cleene, M. D.
- Venue
- European Journal of Biochemistry
- Publisher
- Wiley
- Published
- 1970-01
- Volume
- 12
- Issue
- 1
- Pages
- 143-153
- ISSN
- 0014-2956 (ISSN-L)
- 1432-1033
- OpenAlex
- W1986549895
- Cited by
- 431
- References
- 15
- FWCI
- 19.08
- Type
- journal article
- Language
- en
- Open access
- closed
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
