Boron Induced Oxidative Stress, Antioxidant Defence Response and Changes in Artemisinin Content in Artemisia annua L.

Abstract

Boron is an essential plant micronutrient and the range between deficient and toxic levels of boron is narrow for most of the plants. Like other elements, boron becomes toxic to growth at high concentrations. High boron concentrations in soil reduce crop productivity in many areas of the world. The effect of increasing levels of boron (0, 0.50, 1.00, 1.50, 2.00 m m) on oxidative stress, antioxidant defence response and changes in artemisinin content in Artemisia annua were investigated in the present study. Boron toxicity reduced the growth parameters viz. stem height, fresh weight and dry weight. Treatments induced oxidative stress resulting in lower net photosynthetic rate, stomatal conductance, internal CO 2 and total chlorophyll content. The increased activities of antioxidant enzymes like CAT, POX and SOD were also noted in response to increasing levels of boron stress. However, H 2 O 2 and artemisinin content were found to be high up to 1.00 m m concentration of boron compared to control, and on applying higher doses, further reduced contents were obtained. Thus, the results suggest that a mild stress of boron can be utilized for enhanced artemisinin production.