Exogenous abscisic acid mediates ROS homeostasis and maintains glandular trichome to enhance artemisinin biosynthesis in Artemisia annua under copper toxicity
Abstract
One of the major abiotic stresses that cause environmental pollution is heavy metal stress. In the present investigation, copper (Cu) toxicity caused morphological and cellular damages to the Artemisia annua L. plants but supplementation of abscisic acid (ABA) ameliorated the damaging effect of Cu. Copper toxicity significantly reduced the shoot and root lengths; fresh and dry weights of shoot. However, exogenous application of ABA to Cu-treated plants significantly attenuated the damaging effects on plants caused by Cu toxicity. Copper stress also reduced the physiological and biochemical parameters, but ABA application ameliorated the negative effects of Cu in the affected plant. Accumulation of Cu in plant tissues significantly increased the membrane damage and oxidative enzyme activities such as catalase (CAT), peroxidase (POX) and superoxide dismutase (SOD). Further, the impact of high concentration of Cu on density, area and ultrastructure of glandular trichomes and artemisinin content was studied. Moreover, the foliar application of ABA improved the area, density of glandular trichomes and secured the plant cells from Cu toxicity. Therefore, this investigation indicated that the exogenous application of ABA protects A. annua plant by increasing antioxidant enzymes activity, which helps in maintaining cell integrity of leaves and results in increased artemisinin production.
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Abscisic acid positively affects artemisinin content and yield in plants as well as in suspension cultures by increasing expression of key artemisinin biosynthetic genes including ADS, CYP71AV1, DBR2 and ALDH1.
“Abscisic acid positively affects artemisinin content and yield in plants as well as in suspension cultures by increasing expression of certain key artemisinin biosynthetic genes including ADS, CYP71AV1, DBR2 and ALDH1 (Zehra et al. 2020; Zebarjadi et al. 2018; Jing et al. 2009).”
From Enhancing artemisinin content in and delivery from Artemisia annua: a review of alternative, classical, and transgenic approaches (2021) · cites this paper as Zehra et al. 2020
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- Authors
- Zehra, A., Choudhary, S., Wani, K. I., Naeem, M., Khan, M. M. A., & Aftab, T.
- Affiliations
- Aligarh Muslim University (IN)
- Corresponding author
- Aftab, Tariq
- Venue
- Plant Physiology and Biochemistry
- Publisher
- Elsevier BV
- Published
- 2020-11
- Volume
- 156
- Pages
- 125-134
- ISSN
- 0981-9428 (ISSN-L)
- OpenAlex
- W3084389578
- Cited by
- 67
- References
- 100
- Citations by year
- 2026: 6 · 2025: 13 · 2024: 13 · 2023: 12 · 2022: 11 · 2021: 12
- FWCI
- 5.65
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Plant Physiology and Biochemistry (publishedVersion)
- PubMed (publishedVersion)
- Funders
- University Grants Commission
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Stress Responses and Tolerance
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant-Microbe Interactions and Immunity
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Growth Enhancement Techniques
- Keywords
- abscisic acid, copper toxicity, heavy metal stress, Artemisia annua, artemisinin biosynthesis, glandular trichomes, reactive oxygen species homeostasis, antioxidant enzymes, oxidative stress, stress reduction, foliar application
- MeSH terms
- Abscisic Acid, Copper, Homeostasis, Reactive Oxygen Species, Plant Leaves, Artemisia annua, Artemisinins, Trichomes
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