The DOI matches its Crossref record; 57 of the 57 references it cites were found in a registry or an index.
The Pseudomonas aeruginosa sensor RetS switches Type III and Type VI secretion via c‐di‐GMP signalling
Abstract
Summary: Acute bacterial infections are associated with motility and cytotoxicity via the type III secretion system (T3SS), while chronic infections are linked to biofilm formation and reduced virulence. In Pseudomonas aeruginosa , the transition between motility and sessility involves regulatory networks including the RetS/GacS sensors, as well as the second messenger c‐di‐GMP. The RetS/GacS signalling cascade converges on small RNAs, RsmY and RsmZ, which control a range of functions via RsmA. A retS mutation induces biofilm formation, and high levels of c‐di‐GMP produce a similar response. In this study, we connect RetS and c‐di‐GMP pathways by showing that the retS mutant displays high levels of c‐di‐GMP. Furthermore, a retS mutation leads to repression of the T3SS, but also upregulates the type VI secretion system (T6SS), which is associated with chronic infections. Strikingly, production of the T3SS and T6SS can be switched by artificially modulating c‐di‐GMP levels. We show that the diguanylate cyclase WspR is specifically involved in the T3SS/T6SS switch and that RsmY and RsmZ are required for the c‐di‐GMP‐dependent response. These results provide a firm link between the RetS/GacS and the c‐di‐GMP pathways, which coordinate bacterial lifestyles, as well as secretion systems that determine the infection strategy of P. aeruginosa .
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Cites
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Cited by
Pseudomonas aeruginosa Lifestyle: A Paradigm for Adaptation, Survival, and Persistence
Moradali, M. F., Ghods, S., & Rehm, B. H. A. (2017) · Frontiers in Cellular and Infection Microbiology · doi:10.3389/fcimb.2017.00039 · Cited by 1,537
Pseudomonas aeruginosa : new insights into pathogenesis and host defenses
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Valentini, M., & Filloux, A. (2016) · Journal of Biological Chemistry · doi:10.1074/jbc.r115.711507 · Cited by 668
Self-citationThe role of RNA regulators, quorum sensing and c‐di‐GMP in bacterial biofilm formation
Condinho, M., Carvalho, B., Cruz, A., Pinto, S. N., Arraiano, C. M., & Pobre, V. (2023) · FEBS Open Bio · doi:10.1002/2211-5463.13389 · Cited by 64
Autoinducer-2 and bile salts induce c-di-GMP synthesis to repress the T3SS via a T3SS chaperone
Li, S., Sun, H., Li, J., Zhao, Y., Wang, R., Xu, L., Duan, C., Li, J., Wang, Z., Liu, Q., et al. (2022) · Nature Communications · doi:10.1038/s41467-022-34607-9 · Cited by 58
Metadata
- Authors
- Moscoso, J. A., Mikkelsen, H., Heeb, S., Williams, P., & Filloux, A.
- Venue
- Environmental Microbiology
- Publisher
- Wiley
- Published
- 2011-09-29
- Volume
- 13
- Issue
- 12
- Pages
- 3128-3138
- ISSN
- 1462-2912 (ISSN-L)
- 1462-2920
- OpenAlex
- W2171351196
- Cited by
- 284
- References
- 57
- FWCI
- 6.82
- Type
- journal article
- Language
- en
- Open access
- closed
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- Moscoso, J. A., Mikkelsen, H., Heeb, S., Williams, P., & Filloux, A. (2011). The Pseudomonas aeruginosa sensor RetS switches Type III and Type VI secretion via c‐di‐GMP signalling. Environmental Microbiology. https://doi.org/10.1111/j.1462-2920.2011.02595.x
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