The DOI matches its Crossref record; 39 of the 39 references it cites were found in a registry or an index.
Development of intracellular bacterial communities of uropathogenic Escherichia coli depends on type 1 pili
Open accessAbstract
Uropathogenic Escherichia coli, the predominant causative agent of urinary tract infections, use type 1 pili to bind and invade bladder epithelial cells. Upon entry, the bacteria rapidly replicate and enter a complex developmental pathway ultimately forming intracellular bacterial communities (IBCs), a niche with biofilm-like properties protected from innate defences and antibiotics. Paradoxically, bacteria within IBCs produce type 1 pili, an organelle thought only to be an extracellular colonization factor. Thus, we investigated the function of type 1 pili in IBC development. The cystitis isolate, UTI89, was genetically manipulated for conditional fim expression under control of the tet promoter. In this strain, UTI89-tetR/P(tet) fim, piliation is constitutively inhibited by the tetracycline repressor, TetR. Repression is relieved by anhydrotetracycline (AHT) treatment. UTI89-tetR/P(tet) fim and the isogenic control strain, UTI89-tetR, grown in the presence of AHT, colonized the bladder and invaded the superficial umbrella cells at similar levels at early times in a murine model of infection. However, after invasion UTI89-tetR/P(tet) fim became non-piliated and was unable to form typical IBCs comprised of tightly packed, coccoid-shaped bacteria in contrast to the control strain, UTI89-tetR. Thus, this work changes the extracellular colonization functional paradigm of pili by demonstrating their intracellular role in biofilm formation.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products
Datsenko, K. A., & Wanner, B. L. (2000) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.120163297 · Cited by 14,782
Antibiotic resistance of bacteria in biofilms
Stewart, P. S., & William Costerton, J. (2001) · The Lancet · doi:10.1016/s0140-6736(01)05321-1 · Cited by 4,585
Costerton, J. W., Lewandowski, Z., Caldwell, D. E., Korber, D. R., & Lappin-Scott, H. M. (1995) · Annual Review of Microbiology · doi:10.1146/annurev.mi.49.100195.003431 · Cited by 4,544
Epidemiology of urinary tract infections: incidence, morbidity, and economic costs
Foxman, B. (2002) · The American Journal of Medicine · doi:10.1016/s0002-9343(02)01054-9 · Cited by 1,898
Survival strategies of infectious biofilms
Fux, C. A., Costerton, J. W., Stewart, P. S., & Stoodley, P. (2005) · Trends in Microbiology · doi:10.1016/j.tim.2004.11.010 · Cited by 1,812
Lutz, R. (1997) · Nucleic Acids Research · doi:10.1093/nar/25.6.1203 · Cited by 1,693
Pratt, L. A., & Kolter, R. (1998) · Molecular Microbiology · doi:10.1046/j.1365-2958.1998.01061.x · Cited by 1,636
Intracellular Bacterial Biofilm-Like Pods in Urinary Tract Infections
Anderson, G. G., Palermo, J. J., Schilling, J. D., Roth, R., Heuser, J., & Hultgren, S. J. (2003) · Science · doi:10.1126/science.1084550 · Cited by 1,161
Self-citationAmann, E., Ochs, B., & Abel, K.-J. (1988) · Gene · doi:10.1016/0378-1119(88)90440-4 · Cited by 1,061
Induction and Evasion of Host Defenses by Type 1-Piliated Uropathogenic Escherichia coli
Mulvey, M. A., Lopez-Boado, Y. S., Wilson, C. L., Roth, R., Parks, W. C., Heuser, J., & Hultgren, S. J. (1998) · Science · doi:10.1126/science.282.5393.1494 · Cited by 947
Self-citation
Cited by
Secretion systems in Gram-negative bacteria: Structural and mechanistic insights
Costa, T. R. D., Felisberto-Rodrigues, C., Meir, A., Prevost, M. S., Redzej, A., Trokter, M., & Waksman, G. (2015) · Nature Reviews Microbiology · doi:10.1038/nrmicro3456 · Cited by 1,132
The biology and future prospects of antivirulence therapies
Cegelski, L., Marshall, G. R., Eldridge, G. R., & Hultgren, S. J. (2008) · Nature Reviews Microbiology · doi:10.1038/nrmicro1818 · Cited by 788
Self-citationDetection of Intracellular Bacterial Communities in Human Urinary Tract Infection
Rosen, D. A., Hooton, T. M., Stamm, W. E., Humphrey, P. A., & Hultgren, S. J. (2007) · PLoS Medicine · doi:10.1371/journal.pmed.0040329 · Cited by 651
“UPEC express adhesive fibers known as type 1 pili that mediate binding to and invasion of luminal facet cells of the bladder [16–18].”
Self-citationOrigins and virulence mechanisms of uropathogenic Escherichia coli
Wiles, T. J., Kulesus, R. R., & Mulvey, M. A. (2008) · Experimental and Molecular Pathology · doi:10.1016/j.yexmp.2008.03.007 · Cited by 606
A central metabolic circuit controlled by QseC in pathogenic Escherichia coli
Hadjifrangiskou, M., Kostakioti, M., Chen, S. L., Henderson, J. P., Greene, S. E., & Hultgren, S. J. (2011) · Molecular Microbiology · doi:10.1111/j.1365-2958.2011.07660.x · Cited by 118
Self-citation
Metadata
- Authors
- Wright, K. J., Seed, P. C., & Hultgren, S. J.
- Venue
- Cellular Microbiology
- Publisher
- Hindawi Limited
- Published
- 2007-09
- Volume
- 9
- Issue
- 9
- Pages
- 2230-2241
- ISSN
- 1462-5814 (ISSN-L)
- 1462-5822
- OpenAlex
- W2119836979
- Cited by
- 334
- References
- 39
- FWCI
- 12.73
- Type
- journal article
- Language
- en
- Open access
- bronze
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Development of intracellular bacterial communities of uropathogenic Escherichia coli depends on type 1 pili. https://app.cochat.ai/papers/10.1111/j.1462-5822.2007.00952.x
- The paper
- Wright, K. J., Seed, P. C., & Hultgren, S. J. (2007). Development of intracellular bacterial communities of uropathogenic Escherichia coli depends on type 1 pili. Cellular Microbiology. https://doi.org/10.1111/j.1462-5822.2007.00952.x
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
